Click here to switch to the map view.

The map label for this gene is brnQ5 [H]

Identifier: 227814063

GI number: 227814063

Start: 1349373

End: 1350725

Strand: Direct

Name: brnQ5 [H]

Synonym: BAMEG_1470

Alternate gene names: 227814063

Gene position: 1349373-1350725 (Clockwise)

Preceding gene: 227814062

Following gene: 227814064

Centisome position: 25.8

GC content: 33.41

Gene sequence:

>1353_bases
ATGTCAAATAAAGTTCCTACTTCTTTTATCATTATTATAGGATTGATGCTGTTTGCATTGTTTTTTGGAGCAGGAAATTT
AATTTTTCCACCTATGCTTGGTCAAATGGCTGGAAATAATGTATGGATAGCCAATGCAGGCTTTTTAGTTACTGGAGTTG
GTTTACCATTGTTAGCAATAACAGCATTTGTTTTTTCAGGTGAAAGTGATTTGCAATCTTTAGCTAGTCGTGTACATCCG
GTGTTTGGTATTGTATTTGCGATCGTTCTCTATCTAGCGATTGGTCCCTTTTATGCCATCCCTAGATCTGGAACTGTATC
ATTTGAAATTGGAATTAAACCGTTTTTATCAAATGATGCAGGTCCTGCTGCATTAACCGTATTTACAATCTTATTTTTCA
TAGTTACATGCTTATTATCCCTTAATCCTACAAAACTTGTTGATATAGTTGGGAAAATTTTGACTCCTATCAAATTGACT
GTTATTGGGATGCTCGTAGTTGTTGCTTTTATTCACCCAATTGGAAGAATTCAGGCACCTAGTGAAGTGTATGCATCAGA
TGCATTTTTTAAAGGTTTTCAAGAAGGGTATTTAACATTAGATGCTCTTGGAGCTTTTGTTTTCGGAATTATTATTGTGA
ATGCAATTAGAGAAAAAGGGGCTACTACTAAAAAGCAGCTTATGGTTGTTTGTGTAAAAGCAACGACTATTGCTGCTACA
CTCTTAGCTCTTATCTATACTGCCCTTTCCTATATGGGCGCTTCTAGTGTAGAAAAACTAGGTCATTTAAATAATGGGGC
TGAAGTTTTAGCAAAAGTTTCGGACTATTACTTTGGTTCGTATGGTGCAATTTTCTTAGGTGTAATGATTATAGTAGCAT
GTTTAATCACAAGCGTAGGACTTATCACTGCTTGTTCTTCTTTTTTTCATAAATTATTTCCAAAGATTTCTTACAAAAAA
CTTGCTATTATTTTGTCTGTTTTCAGTACACTAGTTGCGAATATAGGGTTAACACAATTAATTAAGATTTCAATCCCTGT
ATTAATAACTATGTATCCAATTGCTATTACCTTAATATTTCTAACATTTTTACACTCTGTATTCAATGGTAGATTTGAAG
TATATCAAGGGAGTTTGATATTGACATTTATTTTTAGTTCGTTAGATGGATTAAAAGCTGCTGGAATAGAAAGCGAAATA
ATCCATAATTTTTTTGAGCACTTTCTTCCTTTATATAGTGTAGGTTTAGGGTGGGGATTTCCGGCTATTATAGGCGGTGT
ATGTGGGTATGTTGTTAGTGTATTACGGAAGAAAAATACCTTAAGTTCTAGTGAAACTCAATTAAATAAATAA

Upstream 100 bases:

>100_bases
TTGCCTTTCACAATGGCGCTCTAGTCTGGAATAAACTAGATTAATATATATTATTTGGAAAATAAAGAGCGTATACAAAT
GTATAATCGGAGGAGTTGTT

Downstream 100 bases:

>100_bases
ATTATTTTTGTTTATCATACCATTATCGGGCATACAAAACTAATTATCTCTTTGAAATGAAAAAATCTTTTATTTATATC
TTTGGAGGTAAATTGAATTA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAITAFVFSGESDLQSLASRVHP
VFGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDAGPAALTVFTILFFIVTCLLSLNPTKLVDIVGKILTPIKLT
VIGMLVVVAFIHPIGRIQAPSEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAAT
LLALIYTALSYMGASSVEKLGHLNNGAEVLAKVSDYYFGSYGAIFLGVMIIVACLITSVGLITACSSFFHKLFPKISYKK
LAIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIFLTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEI
IHNFFEHFLPLYSVGLGWGFPAIIGGVCGYVVSVLRKKNTLSSSETQLNK

Sequences:

>Translated_450_residues
MSNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAITAFVFSGESDLQSLASRVHP
VFGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDAGPAALTVFTILFFIVTCLLSLNPTKLVDIVGKILTPIKLT
VIGMLVVVAFIHPIGRIQAPSEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAAT
LLALIYTALSYMGASSVEKLGHLNNGAEVLAKVSDYYFGSYGAIFLGVMIIVACLITSVGLITACSSFFHKLFPKISYKK
LAIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIFLTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEI
IHNFFEHFLPLYSVGLGWGFPAIIGGVCGYVVSVLRKKNTLSSSETQLNK
>Mature_449_residues
SNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAITAFVFSGESDLQSLASRVHPV
FGIVFAIVLYLAIGPFYAIPRSGTVSFEIGIKPFLSNDAGPAALTVFTILFFIVTCLLSLNPTKLVDIVGKILTPIKLTV
IGMLVVVAFIHPIGRIQAPSEVYASDAFFKGFQEGYLTLDALGAFVFGIIIVNAIREKGATTKKQLMVVCVKATTIAATL
LALIYTALSYMGASSVEKLGHLNNGAEVLAKVSDYYFGSYGAIFLGVMIIVACLITSVGLITACSSFFHKLFPKISYKKL
AIILSVFSTLVANIGLTQLIKISIPVLITMYPIAITLIFLTFLHSVFNGRFEVYQGSLILTFIFSSLDGLKAAGIESEII
HNFFEHFLPLYSVGLGWGFPAIIGGVCGYVVSVLRKKNTLSSSETQLNK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=445, Percent_Identity=40.6741573033708, Blast_Score=320, Evalue=9e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48324; Mature: 48193

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
SNKVPTSFIIIIGLMLFALFFGAGNLIFPPMLGQMAGNNVWIANAGFLVTGVGLPLLAI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHCCCCEEEEECCHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]