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The map label for this gene is hemK

Identifier: 227082303

GI number: 227082303

Start: 2259917

End: 2260777

Strand: Reverse

Name: hemK

Synonym: VCM66_2101

Alternate gene names: 227082303

Gene position: 2260777-2259917 (Counterclockwise)

Preceding gene: 227082304

Following gene: 227082302

Centisome position: 78.16

GC content: 51.92

Gene sequence:

>861_bases
ATGTCAGTTACCATTGAAGCGGCATTAAAAGCGGCAACCGAGCAACTGCAGCAAAGCGGCAGTGATTCGCCAGCCCTAGA
TGCCGCAGTACTCCTTTGCCATGTACTGGCTAAGCCTCGTTCTTACTTGTTGACTTGGCCTGATAAAATCTTGGAGAAGC
CTACTTTGGCTTCTCTCGAGCTGCTCCTTGCTCGCCGCAGAGCGGGAGAGCCGATGGCCTACATCCTTGGTGAGCGCGAA
TTTTGGTCGCTGCCGCTGAAAGTCTCTCCTTCGACACTTATTCCACGTCCGGATACGGAACGTTTGGTTGAATTAGCGCT
GGATAAAGCGGCGTTGATTGATGGCGAACTGCTGGATCTTGGCACCGGAACTGGGGCGATTGCGTTGGCTTTGGCTTCGG
AATTGCCCACGCGGCAAGTCACGGGGATTGATTTACGTCCGGAGGCGGCAGAGTTAGCAAGAGAAAACGCGACTCGCTTG
GCGATCCACAATGCGCAGTTTTTTCAGGGCAGTTGGTTTAGCCCATTAGCAGACGGTACGAAGTTTGCTTTGATCGTCTC
CAACCCGCCTTATATCGAAGAAAATGATCCGCATTTGAGTCTGGGCGATGTGCGTTTTGAGCCGAAAAGCGCGCTGGTTG
CCGCAGAAAATGGTTTGGCGGACATTCGCCACATCAGCACGCACGCGCCGCATTTTTTGCTCGATGGCGGCTGGCTTTTG
TTTGAGCATGGTTACGATCAAGGCGTTGCGGTGCGCACGATTTTGCGTGATTTAGGCTATCAAAACATCATTACCGAGCA
AGATTATGCCGGTCATGATCGAGTTACACTGGGACAATATAAAACGGAAAGAGAAGCATAA

Upstream 100 bases:

>100_bases
GACCGAAGTGATGGAAGGGGATATGCAATCCTTGATTGACCCTGTGATTCACGAGCATCAAGCGGATCAATTGGCGGCAC
TGGCGGATCAAAACTAATCC

Downstream 100 bases:

>100_bases
TGTACGAAGGATTAAAACATTTTCACCTGTTCACTATCGGCGTCAGCGTAGTCATGCTGTCGATTCGCTATGTGCTCATG
ATGATGAATTCAGCACACCT

Product: hemK protein

Products: NA

Alternate protein names: M.VchAHemK2P

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLELLLARRRAGEPMAYILGERE
FWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDLGTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRL
AIHNAQFFQGSWFSPLADGTKFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPHFLLDGGWLL
FEHGYDQGVAVRTILRDLGYQNIITEQDYAGHDRVTLGQYKTEREA

Sequences:

>Translated_286_residues
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLELLLARRRAGEPMAYILGERE
FWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDLGTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRL
AIHNAQFFQGSWFSPLADGTKFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPHFLLDGGWLL
FEHGYDQGVAVRTILRDLGYQNIITEQDYAGHDRVTLGQYKTEREA
>Mature_285_residues
SVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLELLLARRRAGEPMAYILGEREF
WSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDLGTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLA
IHNAQFFQGSWFSPLADGTKFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPHFLLDGGWLLF
EHGYDQGVAVRTILRDLGYQNIITEQDYAGHDRVTLGQYKTEREA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=266, Percent_Identity=34.2105263157895, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787463, Length=272, Percent_Identity=53.6764705882353, Blast_Score=273, Evalue=7e-75,
Organism=Escherichia coli, GI87082085, Length=185, Percent_Identity=31.8918918918919, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324266, Length=178, Percent_Identity=31.4606741573034, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24582226, Length=282, Percent_Identity=32.2695035460993, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_VIBCH (Q9KQ26)

Other databases:

- EMBL:   AE003852
- PIR:   A82109
- RefSeq:   NP_231809.1
- ProteinModelPortal:   Q9KQ26
- SMR:   Q9KQ26
- GeneID:   2613314
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2178
- TIGR:   VC_2178
- HOGENOM:   HBG732041
- OMA:   DPRPETE
- ProtClustDB:   PRK09328
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 31318; Mature: 31187

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 144-144 BINDING 172-172 BINDING 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLE
CCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHH
LLLARRRAGEPMAYILGEREFWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDL
HHHHHHHCCCCCEEEECCCHHEECCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
GTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLAIHNAQFFQGSWFSPLADGT
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCC
KFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPHFLLDGGWLL
EEEEEECCCCCCCCCCCCEEECCEEECCCCEEEEECCCCHHHHHHHCCCCEEEEECCEEE
FEHGYDQGVAVRTILRDLGYQNIITEQDYAGHDRVTLGQYKTEREA
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
SVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLE
CEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCHHHHH
LLLARRRAGEPMAYILGEREFWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDL
HHHHHHHCCCCCEEEECCCHHEECCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
GTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLAIHNAQFFQGSWFSPLADGT
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCC
KFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPHFLLDGGWLL
EEEEEECCCCCCCCCCCCEEECCEEECCCCEEEEECCCCHHHHHHHCCCCEEEEECCEEE
FEHGYDQGVAVRTILRDLGYQNIITEQDYAGHDRVTLGQYKTEREA
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301