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The map label for this gene is ushA

Identifier: 227082299

GI number: 227082299

Start: 2255632

End: 2257293

Strand: Reverse

Name: ushA

Synonym: VCM66_2097

Alternate gene names: 227082299

Gene position: 2257293-2255632 (Counterclockwise)

Preceding gene: 227082300

Following gene: 227082298

Centisome position: 78.04

GC content: 48.19

Gene sequence:

>1662_bases
ATGAAACAAGGCCTCATTCTAAAATCCGTCCTCAGTGCAGCCATTATTGCCTCTTTGGCAGGTTGTGCAACTGCACCCGC
TCAGCAGTGGGAAGCAGACAAAACCTACAAACTCACTATTCTGCACACCAACGACCACCATGGCCGTTTCTGGCAAAACC
AGTATGGTGAATATGGCATGGCAGCACGTAAAACGCTGATTGATCAACTGCGTGCCGACATTGAAGCTCAAGGTGGCAGC
GTATTACTGCTCTCGGGTGGTGACATCAATACTGGGGTACCAGAATCAGACCTACAAGATGCAGAGCCTGATTTCAAAGG
TATGAGCAAAATCGGTTACGACGCTATGGCGCTGGGTAACCACGAATTTGATAATCCACTTGAAGTGCTGTTTAAGCAAA
AAGAGTGGGCGAACTTCCCAATGCTGTCGGCCAACATTTACGATAAAGCGACCGGTAAGCGTCTGTTTGAACCGTACCAT
ATCTTTGATAAGCAAGGGATTAAGATTGCGGTTATTGGCTTAACCACCGAAGACACCGCGAAAATTGGTAACCCAGAATA
CATCGGTGGTATCGACTTCCGCGATCCGAAAGAAGAAGCGAAGAAAGTGATCGCTGAGCTGAAGAAAAAAGAGAAGCCAG
ATCTTATCATCGCAGTAACGCACATGGGTCACTACCAAAACGGTGAGCATGGTGTGAACGCACCGGGTGATGTTGCTTTG
GCGCGCTACTTACCAGCGGGTGAGTTGGATATGATTGTCGGCGGTCACTCACAAGAGCCTGTGTGTATGGAAGGCCCTAA
TCTGGTGAAAAAGAACTTTAAGCCGGGTGACGAATGTAAGCCTGATATTCAAAACGGCACTTACATTGTTCAAGCTTACG
AGTGGGGTAAATACGTCGGTCGTGCGGATTATGAATTCCGTAACGGTGAGCTGAATATGGTCAGCTATAACCTCATTCCC
GTTAACTTGAAGAAGAAAGTTGAAGTGAATGGTGAAACTCAGCGTGTGTTTGCGACTTCTGAAATTAAAGAAGACAGCGC
AATGCTAGAGTTCCTGCGCCCATTCCAAGAGAAAGGCCAAGAGCAACTGAGCATCAAGATTGCCCACAGTAACGGTAAGC
TAGAAGGCGATCGTAACGTGGTGCGTTTTGAGCAAACCAACTTGGGCCGCATGATTGCAATGGCGCACATGCAACGTGCT
AAAGCCGATTTTGCGGTAATGAACTCTGGTGGCGTACGTGATTCTATCCAAGCCGGTGACATTACGTATAAAGATGTACT
GAAAGTGCAGCCATTTGGCAACATCGTCAGCTACGTGGATATGAATGGTCAGGAAGTGCTGGATTATCTCAATGTCGTCG
CTACCAAGCCGGTCGATTCAGGTGCGTATGCCCAGTTCGCAGGGATTTCAATGACGGTTGCGGATGGCAAAGTCTCTAAC
GTGGTCATCGGTGGCAAACAACTGCGTCTCGATGCGACCTACCGTTTCACTGTGCCAAGTTTTAACGCAGCGGGTGGTGA
CGGTTATCCAAAAATTACTGACCACCCAGGCTATGTCAACACTGGCTTTGTGGATGCCGAAGTACTGAAAGATTACCTTG
AGGCGAACAGTCCAATCGATGTGAACCGTTTCGCGCCAGCGGGCGAGATTGTTTATCGTTAA

Upstream 100 bases:

>100_bases
AAGCAATGTGCTCAAGCGATGTGCTGTGGTAAACGACTGCGCCGTGCGAGCGCCCAACGACATTTCAAGCAGTGGTTTTC
ATCAGTTCAAAGGTATGACA

Downstream 100 bases:

>100_bases
TAAGGTTTGACTCGCAAAGTTGAATTGCGTTTACTGACAGGCGGTGTGCAAACACCGCCTTTTTTGTTTTGCCATACCCA
TATGGCCCGAGCTCTGCCCA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGMAARKTLIDQLRADIEAQGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYH
IFDKQGIKIAVIGLTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVGRADYEFRNGELNMVSYNLIP
VNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQEQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRA
KADFAVMNSGGVRDSIQAGDITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPIDVNRFAPAGEIVYR

Sequences:

>Translated_553_residues
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGMAARKTLIDQLRADIEAQGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYH
IFDKQGIKIAVIGLTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVGRADYEFRNGELNMVSYNLIP
VNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQEQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRA
KADFAVMNSGGVRDSIQAGDITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPIDVNRFAPAGEIVYR
>Mature_553_residues
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGMAARKTLIDQLRADIEAQGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYH
IFDKQGIKIAVIGLTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVGRADYEFRNGELNMVSYNLIP
VNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQEQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRA
KADFAVMNSGGVRDSIQAGDITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPIDVNRFAPAGEIVYR

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family

Homologues:

Organism=Homo sapiens, GI4505467, Length=573, Percent_Identity=26.0034904013962, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=62.2950819672131, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=494, Percent_Identity=21.4574898785425, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922446, Length=538, Percent_Identity=27.3234200743494, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24654424, Length=538, Percent_Identity=27.3234200743494, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922444, Length=581, Percent_Identity=25.1290877796902, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24641187, Length=579, Percent_Identity=24.3523316062176, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19921980, Length=541, Percent_Identity=25.3234750462107, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652512, Length=541, Percent_Identity=25.3234750462107, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161076508, Length=541, Percent_Identity=25.3234750462107, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573524, Length=568, Percent_Identity=24.6478873239437, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI221329836, Length=573, Percent_Identity=23.5602094240838, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): 5NTD_VIBCH (Q9KQ30)

Other databases:

- EMBL:   AE003852
- PIR:   E82108
- RefSeq:   NP_231805.1
- ProteinModelPortal:   Q9KQ30
- SMR:   Q9KQ30
- GeneID:   2613310
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2174
- TIGR:   VC_2174
- HOGENOM:   HBG534106
- OMA:   NDYGEYG
- ProtClustDB:   PRK09558
- BRENDA:   3.1.3.5
- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843
- Gene3D:   G3DSA:3.90.780.10
- PANTHER:   PTHR11575
- PRINTS:   PR01607

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos; SSF55816 5'-Nucleotdase_C

EC number: =3.1.3.5

Molecular weight: Translated: 60904; Mature: 60904

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00785 5_NUCLEOTIDASE_1; PS00786 5_NUCLEOTIDASE_2; PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: BINDING 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGM
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHCCCCCH
AARKTLIDQLRADIEAQGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGN
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECC
HEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYHIFDKQGIKIAVIGLTTEDTA
CCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHCCCHHEECCCCCEEEEEEECCCCHH
KIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
CCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHH
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVG
HHHCCCCCEEEEECCCCCCCEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
RADYEFRNGELNMVSYNLIPVNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQ
CCCCEEECCEEEEEEEEEEEECEEEEEEECCCCEEEEEECHHCHHHHHHHHHHHHHHCCC
EQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRAKADFAVMNSGGVRDSIQAGD
EEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
ITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
CCHHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCE
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPID
EEECCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNRFAPAGEIVYR
CCCCCCCCCEEEC
>Mature Secondary Structure
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGM
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHCCCCCH
AARKTLIDQLRADIEAQGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGN
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECC
HEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYHIFDKQGIKIAVIGLTTEDTA
CCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHCCCHHEECCCCCEEEEEEECCCCHH
KIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
CCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHH
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVG
HHHCCCCCEEEEECCCCCCCEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
RADYEFRNGELNMVSYNLIPVNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQ
CCCCEEECCEEEEEEEEEEEECEEEEEEECCCCEEEEEECHHCHHHHHHHHHHHHHHCCC
EQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRAKADFAVMNSGGVRDSIQAGD
EEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
ITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
CCHHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCE
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPID
EEECCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNRFAPAGEIVYR
CCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301