Click here to switch to the map view.

The map label for this gene is menC

Identifier: 227082099

GI number: 227082099

Start: 2060280

End: 2061278

Strand: Reverse

Name: menC

Synonym: VCM66_1896

Alternate gene names: 227082099

Gene position: 2061278-2060280 (Counterclockwise)

Preceding gene: 227082100

Following gene: 227082098

Centisome position: 71.26

GC content: 52.75

Gene sequence:

>999_bases
ATGCGTCATGCAACCCTCTATCGCTACCAACTGCCGATGGATAGCGGTGTCATCCTTCGCAATGAGAAACTGACTCAGCG
TGAAGGCTTTATTGTCGAACTGACCGAAAACGGCCGTACTGCACGCGGCGAGATTGCACCGCTGCCCGGCTTTAGCCGTG
AAACGCTGGAAGACGCCGGCTTACAAGCGCAAGCGCTGCTTGAGCAGTGGGTCAAAGGTCACGCTATCGAGTGGGATGCA
CAGCATCCTTCGGTCGCTTTTGGTCTTTCGATGGCACATTACGAGCTAGAACAAGCGCTGCCCGAGCAAGGCAACTACTA
TGTTGCGCCGCTGTGTACGGGCGATCCTGATGAGCTGCTGCCGGTGTTGAACAACTTACCGGGGCAGAAAGTCGCTAAAG
TTAAAGTTGGGCTGTATGAACCGATTCGGGATGGCATGCTCGTCAATCTATTCTTGGAATCGATGCCGGATTTAACGCTG
CGTTTGGATGCCAACCGCGCGTGGACTCCAGCCAAAGCGCTCAAGTTCGCTCAATACGTTGCGCCGTCTTTGCGTAGCCG
AATTGCGTTTTTGGAAGAGCCGTGCCAGTCGCCAAGCGAAAGCATCGCATTTTCGATTGATACCGGGATTGCGATTGCGT
GGGACGAAACCTTACAAGAAGCGGTTCGCGATGCGGATTTTGCTTTAGAGAATCTGTTAGGTGTCAAAACCATTGTCATC
AAACCAACCCTAATTGGCTCGGTGTATCGTGTAGAGGCTTTGATCGAGAAAGCCAAAACACTTGGGCTACAAGCGGTGAT
CAGCTCTAGCTTAGAGTCGAGCTTGGGCTTGAACCAGTTAGCACGTTTAGCGCATAAACTGCTGCCTAATGAAGTGCCGG
GACTGGATACGATTGGGTTATTCCGAGCTCAGCTCGAAACGCCTTGGCCAAATAGCTCACTGCCTGTGGTTGCCCTGCAA
GAGCAGTCAATCGTATGGCGCTCGGAATCTTCCCTGTGA

Upstream 100 bases:

>100_bases
GCGTAACCCATAATTCTTCTCAGAGCGGCGTCCTGCCGCTCTTTCTCTTTGAGCATAAACCCCGTTTATGTGGATGTATT
GGATATAGGAAGAGATCAGC

Downstream 100 bases:

>100_bases
GCCTTTGGCAGCCTTGGCTTGAGCGTGACCCCAAGCAGGTTGCGCTGAGTCATGCTGGGCAAACCCTGACTTGGCAAGTA
TTAGATAGCCAAGTAGCGCA

Product: O-succinylbenzoate synthase

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRHATLYRYQLPMDSGVILRNEKLTQREGFIVELTENGRTARGEIAPLPGFSRETLEDAGLQAQALLEQWVKGHAIEWDA
QHPSVAFGLSMAHYELEQALPEQGNYYVAPLCTGDPDELLPVLNNLPGQKVAKVKVGLYEPIRDGMLVNLFLESMPDLTL
RLDANRAWTPAKALKFAQYVAPSLRSRIAFLEEPCQSPSESIAFSIDTGIAIAWDETLQEAVRDADFALENLLGVKTIVI
KPTLIGSVYRVEALIEKAKTLGLQAVISSSLESSLGLNQLARLAHKLLPNEVPGLDTIGLFRAQLETPWPNSSLPVVALQ
EQSIVWRSESSL

Sequences:

>Translated_332_residues
MRHATLYRYQLPMDSGVILRNEKLTQREGFIVELTENGRTARGEIAPLPGFSRETLEDAGLQAQALLEQWVKGHAIEWDA
QHPSVAFGLSMAHYELEQALPEQGNYYVAPLCTGDPDELLPVLNNLPGQKVAKVKVGLYEPIRDGMLVNLFLESMPDLTL
RLDANRAWTPAKALKFAQYVAPSLRSRIAFLEEPCQSPSESIAFSIDTGIAIAWDETLQEAVRDADFALENLLGVKTIVI
KPTLIGSVYRVEALIEKAKTLGLQAVISSSLESSLGLNQLARLAHKLLPNEVPGLDTIGLFRAQLETPWPNSSLPVVALQ
EQSIVWRSESSL
>Mature_332_residues
MRHATLYRYQLPMDSGVILRNEKLTQREGFIVELTENGRTARGEIAPLPGFSRETLEDAGLQAQALLEQWVKGHAIEWDA
QHPSVAFGLSMAHYELEQALPEQGNYYVAPLCTGDPDELLPVLNNLPGQKVAKVKVGLYEPIRDGMLVNLFLESMPDLTL
RLDANRAWTPAKALKFAQYVAPSLRSRIAFLEEPCQSPSESIAFSIDTGIAIAWDETLQEAVRDADFALENLLGVKTIVI
KPTLIGSVYRVEALIEKAKTLGLQAVISSSLESSLGLNQLARLAHKLLPNEVPGLDTIGLFRAQLETPWPNSSLPVVALQ
EQSIVWRSESSL

Specific function: Converts SHCHC to OSB

COG id: COG1441

COG function: function code H; O-succinylbenzoate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1788596, Length=317, Percent_Identity=56.4668769716088, Blast_Score=348, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENC_VIBCH (Q9KQM6)

Other databases:

- EMBL:   AE003852
- PIR:   F82134
- RefSeq:   NP_231606.1
- ProteinModelPortal:   Q9KQM6
- SMR:   Q9KQM6
- GeneID:   2613476
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1972
- TIGR:   VC_1972
- HOGENOM:   HBG644552
- OMA:   YEANRDG
- ProtClustDB:   PRK05105
- BRENDA:   4.2.1.113
- HAMAP:   MF_00470
- InterPro:   IPR013342
- InterPro:   IPR010196
- SMART:   SM00922
- TIGRFAMs:   TIGR01927

Pfam domain/function: PF01188 MR_MLE

EC number: =4.2.1.113

Molecular weight: Translated: 36647; Mature: 36647

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: ACT_SITE 135-135 ACT_SITE 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHATLYRYQLPMDSGVILRNEKLTQREGFIVELTENGRTARGEIAPLPGFSRETLEDAG
CCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCC
LQAQALLEQWVKGHAIEWDAQHPSVAFGLSMAHYELEQALPEQGNYYVAPLCTGDPDELL
CHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
PVLNNLPGQKVAKVKVGLYEPIRDGMLVNLFLESMPDLTLRLDANRAWTPAKALKFAQYV
HHHHCCCCCEEEEEEECCHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
APSLRSRIAFLEEPCQSPSESIAFSIDTGIAIAWDETLQEAVRDADFALENLLGVKTIVI
HHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEE
KPTLIGSVYRVEALIEKAKTLGLQAVISSSLESSLGLNQLARLAHKLLPNEVPGLDTIGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHH
FRAQLETPWPNSSLPVVALQEQSIVWRSESSL
HHHHCCCCCCCCCCCEEEECCCEEEECCCCCC
>Mature Secondary Structure
MRHATLYRYQLPMDSGVILRNEKLTQREGFIVELTENGRTARGEIAPLPGFSRETLEDAG
CCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCC
LQAQALLEQWVKGHAIEWDAQHPSVAFGLSMAHYELEQALPEQGNYYVAPLCTGDPDELL
CHHHHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
PVLNNLPGQKVAKVKVGLYEPIRDGMLVNLFLESMPDLTLRLDANRAWTPAKALKFAQYV
HHHHCCCCCEEEEEEECCHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
APSLRSRIAFLEEPCQSPSESIAFSIDTGIAIAWDETLQEAVRDADFALENLLGVKTIVI
HHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEE
KPTLIGSVYRVEALIEKAKTLGLQAVISSSLESSLGLNQLARLAHKLLPNEVPGLDTIGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHH
FRAQLETPWPNSSLPVVALQEQSIVWRSESSL
HHHHCCCCCCCCCCCEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301