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The map label for this gene is menE [H]

Identifier: 227082098

GI number: 227082098

Start: 2058874

End: 2060304

Strand: Reverse

Name: menE [H]

Synonym: VCM66_1895

Alternate gene names: 227082098

Gene position: 2060304-2058874 (Counterclockwise)

Preceding gene: 227082099

Following gene: 227082096

Centisome position: 71.23

GC content: 53.25

Gene sequence:

>1431_bases
ATGGCGCTCGGAATCTTCCCTGTGAGCCTTTGGCAGCCTTGGCTTGAGCGTGACCCCAAGCAGGTTGCGCTGAGTCATGC
TGGGCAAACCCTGACTTGGCAAGTATTAGATAGCCAAGTAGCGCACTACGCTAAAGCGCTGCGTGAGCAAGGGGTTCGGT
CGGGGGAAGTGGTGACGCTGGTCGGGAAAAATCATCTGCACACTGTGCTGTGGTTTTTGGCTTGTACTCAAGTGGGCGCG
CTGTGCGCCTTTCTTGCGCCTCAACCTTTAGCTCGTTTGCAGGAAAAGCTCGCCACACTCTATGCCGAGCAAAGCCCGAA
GCACCTTTGGCTAGCGCCTTCTTGCGCGCTGAGTGAAGAAGATATTACGGCGCTTAATGCCCATCGCCTGAGTTTGCCCG
ATGAGCGGGATGAATCCGTTGGTGGCGAGAAAGTCTCTAGCCAGTTTTCTGGTGAACAACTGGCGACATTAATTTTCACT
TCAGGTTCGACGGGGACACCTAAAGCGGTGGCACACACCCATCAGCAACATTTGGTTTCGGCGCGCGGTTTACTGAACAA
GTTTGCTTTTACCGAATCAGACAGTTGGTTGCTGAGCTTGCCGCTGTATCATATCTCTGGTGTCGCTATTCTCTATCGCT
GGCTTGCTGTCGGAGCCACACTCAAGATCGGCACTGGCGATCTGCAAAACGATATTGCTGGCGTCACTCACGCTTCATTG
GTGCCTACTCAGCTTAAACGTCTGTTGGACAGCAGCTCTCCATTGACGCTCAAACGTGTTCTGCTCGGCGGGAGCCATAT
CCCCGTTGAACTCGCTCAAATGGCGGCAACGCGCGGTATTGATACTTGGCTCGGCTATGGCATGACCGAAGCCGCCTCCA
CCGTGACGGCAAAGCGGGTCGATGGACTTGCAGGTAATGGTGAGCTTTTGGAGCATCGTGAGCTGCGCCTTGTTGATGGG
CGGATTTTCATTGCTGGGAAGACGTTGGCACAAGGCTATTATCGGCAAGGGCAGCTAGTGCCGCTCACTAATGAGCAGGG
TTGGTTTGACAGCAAAGATCTCGGTCGCTGGCAGGGCAATAACCTGTTGATTGATGGCCGAGCGGATAATCTTTTTATCT
CTGGCGGTGAAAATGTTCACTGTGAAGAAATAGAAGCGGTGTTAAACCAGCACCCTCATGTGCAAGTGGCGATCGTAATA
CCCGTTCAAGATAGTGAGTTTGGTGCGCGACCCGTCGCCGTGATTCGTAGTGAGGCCGAGTGGTGTCAAGCCATCGGTGA
TACTTGGTGCCAAGGTAAACTCGAAAAATTCAAATGGCCTATCATGTACTGTTTACTCCCCGATGCTTTGCTGGATGGGG
GAATTAAAGTCTCTCGTGCGGCGGTGAAAGCGTGGTTGGCCGCCAACCAAACGCAGTTCACTCCGCTATAA

Upstream 100 bases:

>100_bases
TGCCGGGACTGGATACGATTGGGTTATTCCGAGCTCAGCTCGAAACGCCTTGGCCAAATAGCTCACTGCCTGTGGTTGCC
CTGCAAGAGCAGTCAATCGT

Downstream 100 bases:

>100_bases
GATTAAACCTATCCGCTGCTGGGTGAGTTTATGGTCGCGGGCGAGTCGTCAACCGTAAGTACGCCAATCCGATCACAAGG
CCAATCAGCGTCGCGCTCAT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTLVGKNHLHTVLWFLACTQVGA
LCAFLAPQPLARLQEKLATLYAEQSPKHLWLAPSCALSEEDITALNAHRLSLPDERDESVGGEKVSSQFSGEQLATLIFT
SGSTGTPKAVAHTHQQHLVSARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRVDGLAGNGELLEHRELRLVDG
RIFIAGKTLAQGYYRQGQLVPLTNEQGWFDSKDLGRWQGNNLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVI
PVQDSEFGARPVAVIRSEAEWCQAIGDTWCQGKLEKFKWPIMYCLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL

Sequences:

>Translated_476_residues
MALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTLVGKNHLHTVLWFLACTQVGA
LCAFLAPQPLARLQEKLATLYAEQSPKHLWLAPSCALSEEDITALNAHRLSLPDERDESVGGEKVSSQFSGEQLATLIFT
SGSTGTPKAVAHTHQQHLVSARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRVDGLAGNGELLEHRELRLVDG
RIFIAGKTLAQGYYRQGQLVPLTNEQGWFDSKDLGRWQGNNLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVI
PVQDSEFGARPVAVIRSEAEWCQAIGDTWCQGKLEKFKWPIMYCLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL
>Mature_475_residues
ALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTLVGKNHLHTVLWFLACTQVGAL
CAFLAPQPLARLQEKLATLYAEQSPKHLWLAPSCALSEEDITALNAHRLSLPDERDESVGGEKVSSQFSGEQLATLIFTS
GSTGTPKAVAHTHQQHLVSARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASLV
PTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRVDGLAGNGELLEHRELRLVDGR
IFIAGKTLAQGYYRQGQLVPLTNEQGWFDSKDLGRWQGNNLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVIP
VQDSEFGARPVAVIRSEAEWCQAIGDTWCQGKLEKFKWPIMYCLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=323, Percent_Identity=26.625386996904, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788595, Length=467, Percent_Identity=40.8993576017131, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI145693145, Length=493, Percent_Identity=24.7464503042596, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI221142682, Length=482, Percent_Identity=21.5767634854772, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789201, Length=266, Percent_Identity=27.0676691729323, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=509, Percent_Identity=23.1827111984283, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=493, Percent_Identity=25.3549695740365, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=426, Percent_Identity=25.5868544600939, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=473, Percent_Identity=22.6215644820296, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=23.6434108527132, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51997; Mature: 51866

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTL
CCEEEEEHHHHCHHHHCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VGKNHLHTVLWFLACTQVGALCAFLAPQPLARLQEKLATLYAEQSPKHLWLAPSCALSEE
ECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
DITALNAHRLSLPDERDESVGGEKVSSQFSGEQLATLIFTSGSTGTPKAVAHTHQQHLVS
CCCEECCEECCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHH
ARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHH
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
DGLAGNGELLEHRELRLVDGRIFIAGKTLAQGYYRQGQLVPLTNEQGWFDSKDLGRWQGN
CCCCCCCCHHCCCCEEEECCEEEEECCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCC
NLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVIPVQDSEFGARPVAVIRSEAE
EEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCHHH
WCQAIGDTWCQGKLEKFKWPIMYCLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL
HHHHHHHHHHCCCHHHCCCCEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALGIFPVSLWQPWLERDPKQVALSHAGQTLTWQVLDSQVAHYAKALREQGVRSGEVVTL
CEEEEEHHHHCHHHHCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VGKNHLHTVLWFLACTQVGALCAFLAPQPLARLQEKLATLYAEQSPKHLWLAPSCALSEE
ECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
DITALNAHRLSLPDERDESVGGEKVSSQFSGEQLATLIFTSGSTGTPKAVAHTHQQHLVS
CCCEECCEECCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHH
ARGLLNKFAFTESDSWLLSLPLYHISGVAILYRWLAVGATLKIGTGDLQNDIAGVTHASL
HHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHCCHHHHH
VPTQLKRLLDSSSPLTLKRVLLGGSHIPVELAQMAATRGIDTWLGYGMTEAASTVTAKRV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
DGLAGNGELLEHRELRLVDGRIFIAGKTLAQGYYRQGQLVPLTNEQGWFDSKDLGRWQGN
CCCCCCCCHHCCCCEEEECCEEEEECCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCC
NLLIDGRADNLFISGGENVHCEEIEAVLNQHPHVQVAIVIPVQDSEFGARPVAVIRSEAE
EEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCHHH
WCQAIGDTWCQGKLEKFKWPIMYCLLPDALLDGGIKVSRAAVKAWLAANQTQFTPL
HHHHHHHHHHCCCHHHCCCCEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]