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The map label for this gene is nlpE [H]

Identifier: 227082091

GI number: 227082091

Start: 2050187

End: 2050678

Strand: Direct

Name: nlpE [H]

Synonym: VCM66_1886

Alternate gene names: 227082091

Gene position: 2050187-2050678 (Clockwise)

Preceding gene: 227082090

Following gene: 227082093

Centisome position: 70.88

GC content: 48.17

Gene sequence:

>492_bases
ATGATGAAAAAAAGTATTTTTGCCCTGAGCGCGCTGACTCTCATTCTTGTCGGTTGCGACAACCAACAAGACGCAAAAGT
GGAAGTAGAAAAAGTCGTTGATGTTGCGGCAGCACCTGCTGAGCAATCTGCGGCTCAACCATCAACAGCAAGCGTTGATG
CAGCGCACAATGCTCAAAACAGCCTAGATTGGGCTGGCATCTATCAAGGCACTCTGCCTTGTGCTGATTGCGGTGGCATT
GAAACAGAGCTGACGCTGAACGCGGATGGCACCTACGCGCTAACAGAAAAATACCTCGATAAAGAAGGTGAACCTTTTGC
CTCTCAAGGTACTTTCGTTTGGAATGAAGCTGGCAACATCGTAACACTGCAAACAGGCGACCAAACTGGCAGACAATTCA
TGGTGGGTGAAAACACGCTGTCCCATCTGGATATGGAAGGTAAGGTTATCGAAGGTGAGCTGGCTGAGTTTTACGTGCTA
AGCAAGCAATAA

Upstream 100 bases:

>100_bases
ACAACTTGTCGCTAAAATGACGCGCGTTTGACTAGGACTTAACGGCATGAGTTCATATTAGTCGTGTTTGTGATGGTCAA
CCTTTCTTAACCAAGGATCT

Downstream 100 bases:

>100_bases
ACCGAGCCGCTTATACGGTTTACGAAAAACGCGCCATCCATTGGGCGCGTTTTTTATTGCCTACATTTAAGCTTTGCTTT
TCGTTTTCGCTTCAAAGGCG

Product: lipoprotein

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MMKKSIFALSALTLILVGCDNQQDAKVEVEKVVDVAAAPAEQSAAQPSTASVDAAHNAQNSLDWAGIYQGTLPCADCGGI
ETELTLNADGTYALTEKYLDKEGEPFASQGTFVWNEAGNIVTLQTGDQTGRQFMVGENTLSHLDMEGKVIEGELAEFYVL
SKQ

Sequences:

>Translated_163_residues
MMKKSIFALSALTLILVGCDNQQDAKVEVEKVVDVAAAPAEQSAAQPSTASVDAAHNAQNSLDWAGIYQGTLPCADCGGI
ETELTLNADGTYALTEKYLDKEGEPFASQGTFVWNEAGNIVTLQTGDQTGRQFMVGENTLSHLDMEGKVIEGELAEFYVL
SKQ
>Mature_163_residues
MMKKSIFALSALTLILVGCDNQQDAKVEVEKVVDVAAAPAEQSAAQPSTASVDAAHNAQNSLDWAGIYQGTLPCADCGGI
ETELTLNADGTYALTEKYLDKEGEPFASQGTFVWNEAGNIVTLQTGDQTGRQFMVGENTLSHLDMEGKVIEGELAEFYVL
SKQ

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=158, Percent_Identity=32.2784810126582, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 17408; Mature: 17408

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKSIFALSALTLILVGCDNQQDAKVEVEKVVDVAAAPAEQSAAQPSTASVDAAHNAQN
CCCHHHHHHHHEEEEEECCCCCCCCEEHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCC
SLDWAGIYQGTLPCADCGGIETELTLNADGTYALTEKYLDKEGEPFASQGTFVWNEAGNI
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEHHHHHHCCCCCCCCCCCCEEEECCCCE
VTLQTGDQTGRQFMVGENTLSHLDMEGKVIEGELAEFYVLSKQ
EEEECCCCCCCEEEECCCCHHHCCCCCEEEECCCEEEEEEECC
>Mature Secondary Structure
MMKKSIFALSALTLILVGCDNQQDAKVEVEKVVDVAAAPAEQSAAQPSTASVDAAHNAQN
CCCHHHHHHHHEEEEEECCCCCCCCEEHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCC
SLDWAGIYQGTLPCADCGGIETELTLNADGTYALTEKYLDKEGEPFASQGTFVWNEAGNI
CCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEHHHHHHCCCCCCCCCCCCEEEECCCCE
VTLQTGDQTGRQFMVGENTLSHLDMEGKVIEGELAEFYVLSKQ
EEEECCCCCCCEEEECCCCHHHCCCCCEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]