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The map label for this gene is yhjG [H]

Identifier: 227082075

GI number: 227082075

Start: 2032608

End: 2034071

Strand: Direct

Name: yhjG [H]

Synonym: VCM66_1869

Alternate gene names: 227082075

Gene position: 2032608-2034071 (Clockwise)

Preceding gene: 227082074

Following gene: 227082078

Centisome position: 70.27

GC content: 48.29

Gene sequence:

>1464_bases
ATGTCCACAAAAATAACCGACATCGTCATTATCGGCGCAGGCCCAGTTGGGTTAATGAGTGCCTACTTAGCACAGCGCTG
CGGTTTAAACGCGCTGATCATTGATAAGTCAGCCGAACCACTTCAAGTCGGACGCGCCGATGCGTTAAATGCCCGCACCT
TACAACTGCTCGAAGTCGCGAAGCTTTTTGATGATCTTTACCCTTTAGGCAAGCCGTGTAATACCAGCTCGGTTTGGGCT
AATGGCCATTTTATCTCGCGCCAATCGAGCTGGTGGGAAGCCTTGGAAGGCTGCTTGCACAAACACTTTTTGATGTTAGG
TCAATCGTTTGTTGAGCAGCTATTGGACAAGAAATTACTTGAAGCGAATGCCCCCGTTTGGCGCAACACAAGCGTCGACA
ATATCGAGCTCAATCAAGCAGGCTGCCTAACTCATCTCAGCAATGGTGAGCTTATTCAATCGCGCTTTGTGATTGGTGCG
GATGGGTCTCAATCTTTTGTGCGTCGCCATTTTAATGTGCCTTTTCACATTGTGCGCCCCGAAATTATCTGGGCGGTGAT
TGATGGTGTGATTGACAGTGACTTTCCTAAAGTCCCTGAAATTATTGTGTTCCAAGCAGAAACCTCAGATGTGGCATGGA
TCCCTCGCGAAGGTGAAATCGATCGCTTTTATGTCCGTATGGATACGCACGATTTCACCTTAGAGCAGGCTATCGCCAAA
ATTAACCGGGCAATGGCACCTCATCAATTGGGTTTTAAAGAAGTGGTCTGGTTCTCGCAATTTGCCGTGAAAGAATCGGT
AGCAGAACATTACAGCGTCGATGACCGGGTTTTCCTTGCAGGGGATGCCTGTCACGTACACTCAGTCAACGGTGGACAAG
GATTAAATACTGGGGTTGCTGACGCATTTAACCTGATCTGGAAACTGAGTATGGTGATTAAAGGTCAAGCCTCTCCTGCT
TTATTGAAAAGTTATCACGCTGAACGTCAACCAGTGGCGCAGAGCGTGATAGACAGCTCCGGCGAACTGGTACGCTCAAC
GAAGTTTTCAGCCACCAACACCCATGCGCAAGACTACGTAAAAATCGTTGAAAAGCGTTCAGGTAATATCACGGGAATGG
GCATTCGCTACTCCGAAGAAGGCTTAGCTGGCACTCGAATGTGGGATTTCGTGCTCAATCAAGGTACTGAGATCACTCGT
TTGTACTCACTGCTTGACTACGCAAGATGGACACTGTTCTTGTTTAATGATGACGAATGCGTGGTTGACGTGCCGCCGCA
TGTACATGTAATCCGCATCGGTACCCAATCCGAAAATGCTCAACACTGGACACGTCACGCACCTTATCCGGGACAAGCGG
TATTGGTTCGCCCTGATGCTTACATCGAATGGGTCACTCCACTGCATCAGGTTCACAGTGTGTTGGCAGAAGGTGTGTTG
ACAGAATTCAACTTATCTCACTAG

Upstream 100 bases:

>100_bases
ATGGCCGTGAAGCCTTTACCTCTCACACGCCGCGTCACATTCGTCGTGTGCAAGTGTTAAGTAATCCTCCTTATAACAAT
CCAAGCTTGGAGTCGTACCA

Downstream 100 bases:

>100_bases
TTCAATACCTGCGTTCACTCGATCTTCATCAAGCCCTAAACGGGCTTGGTGACTTTCCCCGGCACTAGAAGCCATCGACA
CACCCATGCGGCTCATTGCG

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSTKITDIVIIGAGPVGLMSAYLAQRCGLNALIIDKSAEPLQVGRADALNARTLQLLEVAKLFDDLYPLGKPCNTSSVWA
NGHFISRQSSWWEALEGCLHKHFLMLGQSFVEQLLDKKLLEANAPVWRNTSVDNIELNQAGCLTHLSNGELIQSRFVIGA
DGSQSFVRRHFNVPFHIVRPEIIWAVIDGVIDSDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTHDFTLEQAIAK
INRAMAPHQLGFKEVVWFSQFAVKESVAEHYSVDDRVFLAGDACHVHSVNGGQGLNTGVADAFNLIWKLSMVIKGQASPA
LLKSYHAERQPVAQSVIDSSGELVRSTKFSATNTHAQDYVKIVEKRSGNITGMGIRYSEEGLAGTRMWDFVLNQGTEITR
LYSLLDYARWTLFLFNDDECVVDVPPHVHVIRIGTQSENAQHWTRHAPYPGQAVLVRPDAYIEWVTPLHQVHSVLAEGVL
TEFNLSH

Sequences:

>Translated_487_residues
MSTKITDIVIIGAGPVGLMSAYLAQRCGLNALIIDKSAEPLQVGRADALNARTLQLLEVAKLFDDLYPLGKPCNTSSVWA
NGHFISRQSSWWEALEGCLHKHFLMLGQSFVEQLLDKKLLEANAPVWRNTSVDNIELNQAGCLTHLSNGELIQSRFVIGA
DGSQSFVRRHFNVPFHIVRPEIIWAVIDGVIDSDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTHDFTLEQAIAK
INRAMAPHQLGFKEVVWFSQFAVKESVAEHYSVDDRVFLAGDACHVHSVNGGQGLNTGVADAFNLIWKLSMVIKGQASPA
LLKSYHAERQPVAQSVIDSSGELVRSTKFSATNTHAQDYVKIVEKRSGNITGMGIRYSEEGLAGTRMWDFVLNQGTEITR
LYSLLDYARWTLFLFNDDECVVDVPPHVHVIRIGTQSENAQHWTRHAPYPGQAVLVRPDAYIEWVTPLHQVHSVLAEGVL
TEFNLSH
>Mature_486_residues
STKITDIVIIGAGPVGLMSAYLAQRCGLNALIIDKSAEPLQVGRADALNARTLQLLEVAKLFDDLYPLGKPCNTSSVWAN
GHFISRQSSWWEALEGCLHKHFLMLGQSFVEQLLDKKLLEANAPVWRNTSVDNIELNQAGCLTHLSNGELIQSRFVIGAD
GSQSFVRRHFNVPFHIVRPEIIWAVIDGVIDSDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTHDFTLEQAIAKI
NRAMAPHQLGFKEVVWFSQFAVKESVAEHYSVDDRVFLAGDACHVHSVNGGQGLNTGVADAFNLIWKLSMVIKGQASPAL
LKSYHAERQPVAQSVIDSSGELVRSTKFSATNTHAQDYVKIVEKRSGNITGMGIRYSEEGLAGTRMWDFVLNQGTEITRL
YSLLDYARWTLFLFNDDECVVDVPPHVHVIRIGTQSENAQHWTRHAPYPGQAVLVRPDAYIEWVTPLHQVHSVLAEGVLT
EFNLSH

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=346, Percent_Identity=27.4566473988439, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54407; Mature: 54276

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKITDIVIIGAGPVGLMSAYLAQRCGLNALIIDKSAEPLQVGRADALNARTLQLLEVA
CCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCHHHHHHHHHH
KLFDDLYPLGKPCNTSSVWANGHFISRQSSWWEALEGCLHKHFLMLGQSFVEQLLDKKLL
HHHHHHCCCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EANAPVWRNTSVDNIELNQAGCLTHLSNGELIQSRFVIGADGSQSFVRRHFNVPFHIVRP
HCCCCCCCCCCCCCEEECCCCCEEECCCCCEEEEEEEEECCCCHHHHHHHCCCCEEEECH
EIIWAVIDGVIDSDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTHDFTLEQAIAK
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHH
INRAMAPHQLGFKEVVWFSQFAVKESVAEHYSVDDRVFLAGDACHVHSVNGGQGLNTGVA
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCHHH
DAFNLIWKLSMVIKGQASPALLKSYHAERQPVAQSVIDSSGELVRSTKFSATNTHAQDYV
HHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHHHHHCCCCCEEHHCCCCCCCCHHHHHH
KIVEKRSGNITGMGIRYSEEGLAGTRMWDFVLNQGTEITRLYSLLDYARWTLFLFNDDEC
HHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCCCE
VVDVPPHVHVIRIGTQSENAQHWTRHAPYPGQAVLVRPDAYIEWVTPLHQVHSVLAEGVL
EEECCCCEEEEEECCCCCCCHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
TEFNLSH
HHCCCCC
>Mature Secondary Structure 
STKITDIVIIGAGPVGLMSAYLAQRCGLNALIIDKSAEPLQVGRADALNARTLQLLEVA
CCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCHHHHHHHHHH
KLFDDLYPLGKPCNTSSVWANGHFISRQSSWWEALEGCLHKHFLMLGQSFVEQLLDKKLL
HHHHHHCCCCCCCCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EANAPVWRNTSVDNIELNQAGCLTHLSNGELIQSRFVIGADGSQSFVRRHFNVPFHIVRP
HCCCCCCCCCCCCCEEECCCCCEEECCCCCEEEEEEEEECCCCHHHHHHHCCCCEEEECH
EIIWAVIDGVIDSDFPKVPEIIVFQAETSDVAWIPREGEIDRFYVRMDTHDFTLEQAIAK
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHH
INRAMAPHQLGFKEVVWFSQFAVKESVAEHYSVDDRVFLAGDACHVHSVNGGQGLNTGVA
HHHHCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCHHH
DAFNLIWKLSMVIKGQASPALLKSYHAERQPVAQSVIDSSGELVRSTKFSATNTHAQDYV
HHHHHHHHHHHHEECCCCHHHHHHHHHHCCHHHHHHHCCCCCEEHHCCCCCCCCHHHHHH
KIVEKRSGNITGMGIRYSEEGLAGTRMWDFVLNQGTEITRLYSLLDYARWTLFLFNDDEC
HHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEECCCCE
VVDVPPHVHVIRIGTQSENAQHWTRHAPYPGQAVLVRPDAYIEWVTPLHQVHSVLAEGVL
EEECCCCEEEEEECCCCCCCHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
TEFNLSH
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]