Click here to switch to the map view.

The map label for this gene is intA [H]

Identifier: 227081905

GI number: 227081905

Start: 1843226

End: 1844461

Strand: Direct

Name: intA [H]

Synonym: VCM66_1697

Alternate gene names: 227081905

Gene position: 1843226-1844461 (Clockwise)

Preceding gene: 227081904

Following gene: 227081917

Centisome position: 63.72

GC content: 44.09

Gene sequence:

>1236_bases
ATGGCAAATAATCTAACCGCAAGGCAAATTCAAACCGCAAAACCTAAGGATAAGCTTTATCGTTTATCTGATGGCGGCAA
TCTCTACTTTTGCGTACGTCCAAGCAATTCTCGCTCATGGCAATTCCGTTATAAACGACCAGGACAAGATAAGATCACTT
ATCTTTCGTTTGGAACCTATCCCGATATGTCTTTGGCGGAAGCTCGAGAGAAAGCCCTTGAGGCTCGCAAGCAGTTAGCG
GATGGTATTGACCCTCAACTCGCTAAAGAAGAACAAAAAGCAAAAACCATTACTGAACAAAATGCGACGTTCAAGTTTGT
CGCTGAGCAATGGAAGGCTACGAAGGAAGGACAAATAAAAGAGAAGACACTGGAAGGGAATTGGCGAAAACTTGAACTTT
ATGCCTTTCCCAAATTGGGCTCGATACCCGTTAGTAAACTTACTGCGCCGATGGCCATCGCTGCATTACGGCCAATAGAG
TCTCAAGGATTACTAGAAACTGTAAAACGCACCGCCCAACTTATGAATGAAATCATGAACTATGCGGTAAATAGCGGTGT
CATTCATGCTAACCCATTAGCTGGCATCCGTGATGTGTTTAAAAAACACAAAGTGGTGCACATGAAAGCCCTGCAACCAC
ATGAAATGCATGATCTTATACGCACCGTTGCTACAGCAAATATCCAGCATGTGACACGATTTTTAATTGAATGGCAACTT
CACACCATGGTTCGGCCTAACGAAGCCTCTGGTGCTCGTTGGGAAGAAATCGACATGGTTAATAAGCTTTGGATTATCCC
TAAAGAGCGAATGAAGATGAACCGCGAGCACGTAGTCCCACTGACAGCACAAACATTGGCTATTCTTGAAGCGATTAAGC
CCATCAGTGGTCATAGAGAATTCATTTTCCCATCTAGCCGTAATCCAAAGGTACCCACCGATTCTGAAACCGCTAATAAA
GCACTTGGCAGAATGGGATTTAAGGATAGAACGACCGCGCACGGTCTTCGTGCTTTGGCAAGTACCACTCTTAATGAACA
GGGCTTTGAACCTGATGTCATTGAAGCCGCATTGGCTCATACTGACAAAAACCAAATACGCAAAGCATACAACCGTACCG
ACTATTTAGACTCTCGTCGAAAGCTTATGAGCTGGTGGAGTGAGCATATCGAAAAATCTAGCTATGGAAGTTACAGTGTG
GCTGGTACTGGATATTTGCACTTACTGAAAGCCTAA

Upstream 100 bases:

>100_bases
CAGGCTGTAACTTAATGTAAATTAAGTAGTTATTAGCCAAAAGTAATACCTATTTTTTGGAGTCCTCATGCTCTAGCCAG
GTATTACTTGAGGGATTGAA

Downstream 100 bases:

>100_bases
AATAAGTAGTAACACACCAGCATGCCTAGGCCATCTAGTGCGCGATGGGACCACATTGATTGGGATGAGAAAGTTTGAAC
CATTCCTGCGAGAGAATGAA

Product: integrase, phage family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MANNLTARQIQTAKPKDKLYRLSDGGNLYFCVRPSNSRSWQFRYKRPGQDKITYLSFGTYPDMSLAEAREKALEARKQLA
DGIDPQLAKEEQKAKTITEQNATFKFVAEQWKATKEGQIKEKTLEGNWRKLELYAFPKLGSIPVSKLTAPMAIAALRPIE
SQGLLETVKRTAQLMNEIMNYAVNSGVIHANPLAGIRDVFKKHKVVHMKALQPHEMHDLIRTVATANIQHVTRFLIEWQL
HTMVRPNEASGARWEEIDMVNKLWIIPKERMKMNREHVVPLTAQTLAILEAIKPISGHREFIFPSSRNPKVPTDSETANK
ALGRMGFKDRTTAHGLRALASTTLNEQGFEPDVIEAALAHTDKNQIRKAYNRTDYLDSRRKLMSWWSEHIEKSSYGSYSV
AGTGYLHLLKA

Sequences:

>Translated_411_residues
MANNLTARQIQTAKPKDKLYRLSDGGNLYFCVRPSNSRSWQFRYKRPGQDKITYLSFGTYPDMSLAEAREKALEARKQLA
DGIDPQLAKEEQKAKTITEQNATFKFVAEQWKATKEGQIKEKTLEGNWRKLELYAFPKLGSIPVSKLTAPMAIAALRPIE
SQGLLETVKRTAQLMNEIMNYAVNSGVIHANPLAGIRDVFKKHKVVHMKALQPHEMHDLIRTVATANIQHVTRFLIEWQL
HTMVRPNEASGARWEEIDMVNKLWIIPKERMKMNREHVVPLTAQTLAILEAIKPISGHREFIFPSSRNPKVPTDSETANK
ALGRMGFKDRTTAHGLRALASTTLNEQGFEPDVIEAALAHTDKNQIRKAYNRTDYLDSRRKLMSWWSEHIEKSSYGSYSV
AGTGYLHLLKA
>Mature_410_residues
ANNLTARQIQTAKPKDKLYRLSDGGNLYFCVRPSNSRSWQFRYKRPGQDKITYLSFGTYPDMSLAEAREKALEARKQLAD
GIDPQLAKEEQKAKTITEQNATFKFVAEQWKATKEGQIKEKTLEGNWRKLELYAFPKLGSIPVSKLTAPMAIAALRPIES
QGLLETVKRTAQLMNEIMNYAVNSGVIHANPLAGIRDVFKKHKVVHMKALQPHEMHDLIRTVATANIQHVTRFLIEWQLH
TMVRPNEASGARWEEIDMVNKLWIIPKERMKMNREHVVPLTAQTLAILEAIKPISGHREFIFPSSRNPKVPTDSETANKA
LGRMGFKDRTTAHGLRALASTTLNEQGFEPDVIEAALAHTDKNQIRKAYNRTDYLDSRRKLMSWWSEHIEKSSYGSYSVA
GTGYLHLLKA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=405, Percent_Identity=45.1851851851852, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788690, Length=392, Percent_Identity=29.8469387755102, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI145693166, Length=400, Percent_Identity=33.75, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46792; Mature: 46661

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNLTARQIQTAKPKDKLYRLSDGGNLYFCVRPSNSRSWQFRYKRPGQDKITYLSFGTY
CCCCCCHHHHHCCCCHHHEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEECCC
PDMSLAEAREKALEARKQLADGIDPQLAKEEQKAKTITEQNATFKFVAEQWKATKEGQIK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCH
EKTLEGNWRKLELYAFPKLGSIPVSKLTAPMAIAALRPIESQGLLETVKRTAQLMNEIMN
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YAVNSGVIHANPLAGIRDVFKKHKVVHMKALQPHEMHDLIRTVATANIQHVTRFLIEWQL
HHHCCCEEECCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTMVRPNEASGARWEEIDMVNKLWIIPKERMKMNREHVVPLTAQTLAILEAIKPISGHRE
HHHCCCCCCCCCCHHHHHHHHHEEECCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCE
FIFPSSRNPKVPTDSETANKALGRMGFKDRTTAHGLRALASTTLNEQGFEPDVIEAALAH
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TDKNQIRKAYNRTDYLDSRRKLMSWWSEHIEKSSYGSYSVAGTGYLHLLKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECC
>Mature Secondary Structure 
ANNLTARQIQTAKPKDKLYRLSDGGNLYFCVRPSNSRSWQFRYKRPGQDKITYLSFGTY
CCCCCHHHHHCCCCHHHEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEECCC
PDMSLAEAREKALEARKQLADGIDPQLAKEEQKAKTITEQNATFKFVAEQWKATKEGQIK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCH
EKTLEGNWRKLELYAFPKLGSIPVSKLTAPMAIAALRPIESQGLLETVKRTAQLMNEIMN
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YAVNSGVIHANPLAGIRDVFKKHKVVHMKALQPHEMHDLIRTVATANIQHVTRFLIEWQL
HHHCCCEEECCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTMVRPNEASGARWEEIDMVNKLWIIPKERMKMNREHVVPLTAQTLAILEAIKPISGHRE
HHHCCCCCCCCCCHHHHHHHHHEEECCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCE
FIFPSSRNPKVPTDSETANKALGRMGFKDRTTAHGLRALASTTLNEQGFEPDVIEAALAH
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TDKNQIRKAYNRTDYLDSRRKLMSWWSEHIEKSSYGSYSVAGTGYLHLLKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]