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The map label for this gene is metC [H]

Identifier: 227081822

GI number: 227081822

Start: 1750992

End: 1752182

Strand: Direct

Name: metC [H]

Synonym: VCM66_1611

Alternate gene names: 227081822

Gene position: 1750992-1752182 (Clockwise)

Preceding gene: 227081821

Following gene: 227081830

Centisome position: 60.54

GC content: 50.46

Gene sequence:

>1191_bases
ATGTCAGAAGGTAAAAAGACCCGCCTCGTTACCGCGGGACGCAACAAAAAATGGACACTTGGCGTGGTTAACCCCCCAGT
ACAACGCGCTTCTACTGTGGTGTTTGATACGGTCGCACAAAAAAACCAAGCGGCGATCAATCGCGCCAACAAAACCCTGT
TTTACGGACGTCGTGGCACCACAACTCACTTCGCGTTTCAAGAAGCAATGGTTGAGATTGAAGGCGGCGCGGGTTGCGTG
CTCTACCCTTGTGGCGCAGCGGCCATCACCAACGCGATTCTCTCTTTTGTTGCGCAAGGTGACCATATCTTGATGGTCGA
TACCTGTTACGAACCCACCCGTGACTTTTGTGAGAAAGTCCTGAAAAAACTTGGTGTATCAACCACCTATTATGATCCTA
TGCTTGGCGAAGGTATTCGCGAGTTGATTCAACCTAATACCAAGGTTCTGTTTACCGAATCACCGGGCTCACTGACCATG
GAAGTGCAGGATATTCCGACACTCTCTCGCATCGCTCACGAGCACGACATCGTTGTCATGTTGGATAACACTTGGGCGGC
TGGGGTACTGTTCTCACCCTTCGAACATGGCGTCGATATTTCCATTCAAGCAGCGACCAAATACATTGTCGGTCATTCTG
ATGTGATGCTCGGTACTGCGGTCGCCAATGAGAAGTGTTGGGCACAACTGCGTGAACACAGCTATTTGATGGGACAATAT
GCCTCACCTGACGATGTGTACATGGCAATGCGTGGATTGCGCACCCTCGATGTACGTTTACGCCAGCATGAACAAAACAG
TTTACAGATTGCCCAGTGGTTGCAAACTCGCCCAGAAGTCGATCATGTTCGCCACCCTGCGTTAGCGACCTGTCCGGGGC
ACGAGTTTTTCGAACGTGATTTTACTGGCGGCAATGGCTTGTTCTCTTTTGTGTTGAAAACGACGCATCCAGAAGCCACA
ACCGCTCTACTCGATGGCATGCAACACTTCAGCATGGGCTTTTCTTGGGGTGGATTTGAAAGTTTGATCTTAGCCCACGA
ACCCAAAGCCTTTACCTCTCTTCGCTCAGTCGCAAACCCTCACTTCTGCGGCACTCTACTCCGGGTTCATATCGGGTTAG
AAGATGTCGAAGATTTAATCGCCGATCTGGAAGCGGGTTTTGCGCGCTACAACCAAATCCTGCATGCATAG

Upstream 100 bases:

>100_bases
TCTTTAACCCATTATTGTGATTTTCTCACTGAGTGGGGTTGCCATCCGGCACGGCACCATGGTTTAAATAACGTTTTGTA
AAGTCAGACAAGGACAGCAG

Downstream 100 bases:

>100_bases
CAGTTTTTCGTTCCATGATCAAAATAGGGGCTTCAGCCCCTATTTGCTTTGATTTTCTGTATTTTCCCAAACCAGCTCTT
GGTGAATCAGAGCTTGTCTG

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSEGKKTRLVTAGRNKKWTLGVVNPPVQRASTVVFDTVAQKNQAAINRANKTLFYGRRGTTTHFAFQEAMVEIEGGAGCV
LYPCGAAAITNAILSFVAQGDHILMVDTCYEPTRDFCEKVLKKLGVSTTYYDPMLGEGIRELIQPNTKVLFTESPGSLTM
EVQDIPTLSRIAHEHDIVVMLDNTWAAGVLFSPFEHGVDISIQAATKYIVGHSDVMLGTAVANEKCWAQLREHSYLMGQY
ASPDDVYMAMRGLRTLDVRLRQHEQNSLQIAQWLQTRPEVDHVRHPALATCPGHEFFERDFTGGNGLFSFVLKTTHPEAT
TALLDGMQHFSMGFSWGGFESLILAHEPKAFTSLRSVANPHFCGTLLRVHIGLEDVEDLIADLEAGFARYNQILHA

Sequences:

>Translated_396_residues
MSEGKKTRLVTAGRNKKWTLGVVNPPVQRASTVVFDTVAQKNQAAINRANKTLFYGRRGTTTHFAFQEAMVEIEGGAGCV
LYPCGAAAITNAILSFVAQGDHILMVDTCYEPTRDFCEKVLKKLGVSTTYYDPMLGEGIRELIQPNTKVLFTESPGSLTM
EVQDIPTLSRIAHEHDIVVMLDNTWAAGVLFSPFEHGVDISIQAATKYIVGHSDVMLGTAVANEKCWAQLREHSYLMGQY
ASPDDVYMAMRGLRTLDVRLRQHEQNSLQIAQWLQTRPEVDHVRHPALATCPGHEFFERDFTGGNGLFSFVLKTTHPEAT
TALLDGMQHFSMGFSWGGFESLILAHEPKAFTSLRSVANPHFCGTLLRVHIGLEDVEDLIADLEAGFARYNQILHA
>Mature_395_residues
SEGKKTRLVTAGRNKKWTLGVVNPPVQRASTVVFDTVAQKNQAAINRANKTLFYGRRGTTTHFAFQEAMVEIEGGAGCVL
YPCGAAAITNAILSFVAQGDHILMVDTCYEPTRDFCEKVLKKLGVSTTYYDPMLGEGIRELIQPNTKVLFTESPGSLTME
VQDIPTLSRIAHEHDIVVMLDNTWAAGVLFSPFEHGVDISIQAATKYIVGHSDVMLGTAVANEKCWAQLREHSYLMGQYA
SPDDVYMAMRGLRTLDVRLRQHEQNSLQIAQWLQTRPEVDHVRHPALATCPGHEFFERDFTGGNGLFSFVLKTTHPEATT
ALLDGMQHFSMGFSWGGFESLILAHEPKAFTSLRSVANPHFCGTLLRVHIGLEDVEDLIADLEAGFARYNQILHA

Specific function: Methionine biosynthesis; third step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=373, Percent_Identity=31.6353887399464, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI299473759, Length=373, Percent_Identity=28.9544235924933, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI25453487, Length=372, Percent_Identity=27.9569892473118, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1789383, Length=386, Percent_Identity=59.3264248704663, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=32.1518987341772, Blast_Score=181, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=31.3672922252011, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=370, Percent_Identity=30.2702702702703, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=370, Percent_Identity=30.8108108108108, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=336, Percent_Identity=45.2380952380952, Blast_Score=311, Evalue=8e-86,
Organism=Saccharomyces cerevisiae, GI6319307, Length=354, Percent_Identity=30.225988700565, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=385, Percent_Identity=25.974025974026, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323333, Length=358, Percent_Identity=25.4189944134078, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321254, Length=415, Percent_Identity=23.1325301204819, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922554, Length=389, Percent_Identity=31.6195372750643, Blast_Score=201, Evalue=9e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 43886; Mature: 43754

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGKKTRLVTAGRNKKWTLGVVNPPVQRASTVVFDTVAQKNQAAINRANKTLFYGRRGT
CCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
TTHFAFQEAMVEIEGGAGCVLYPCGAAAITNAILSFVAQGDHILMVDTCYEPTRDFCEKV
CHHHHHHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
LKKLGVSTTYYDPMLGEGIRELIQPNTKVLFTESPGSLTMEVQDIPTLSRIAHEHDIVVM
HHHHCCCCEECCCHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCHHHHHHCCCCEEEE
LDNTWAAGVLFSPFEHGVDISIQAATKYIVGHSDVMLGTAVANEKCWAQLREHSYLMGQY
ECCCCCCCEEECHHHCCCCEEEEEHEEEEECCCCEEEEHHHCCHHHHHHHHHCHHHHCCC
ASPDDVYMAMRGLRTLDVRLRQHEQNSLQIAQWLQTRPEVDHVRHPALATCPGHEFFERD
CCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCEEECCCHHHHHHC
FTGGNGLFSFVLKTTHPEATTALLDGMQHFSMGFSWGGFESLILAHEPKAFTSLRSVANP
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCHHHHHHHHHCCC
HFCGTLLRVHIGLEDVEDLIADLEAGFARYNQILHA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEGKKTRLVTAGRNKKWTLGVVNPPVQRASTVVFDTVAQKNQAAINRANKTLFYGRRGT
CCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
TTHFAFQEAMVEIEGGAGCVLYPCGAAAITNAILSFVAQGDHILMVDTCYEPTRDFCEKV
CHHHHHHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
LKKLGVSTTYYDPMLGEGIRELIQPNTKVLFTESPGSLTMEVQDIPTLSRIAHEHDIVVM
HHHHCCCCEECCCHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCHHHHHHCCCCEEEE
LDNTWAAGVLFSPFEHGVDISIQAATKYIVGHSDVMLGTAVANEKCWAQLREHSYLMGQY
ECCCCCCCEEECHHHCCCCEEEEEHEEEEECCCCEEEEHHHCCHHHHHHHHHCHHHHCCC
ASPDDVYMAMRGLRTLDVRLRQHEQNSLQIAQWLQTRPEVDHVRHPALATCPGHEFFERD
CCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCEEECCCHHHHHHC
FTGGNGLFSFVLKTTHPEATTALLDGMQHFSMGFSWGGFESLILAHEPKAFTSLRSVANP
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCHHHHHHHHHCCC
HFCGTLLRVHIGLEDVEDLIADLEAGFARYNQILHA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2659970; 11677609 [H]