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The map label for this gene is phoQ [H]

Identifier: 227081791

GI number: 227081791

Start: 1712043

End: 1713431

Strand: Direct

Name: phoQ [H]

Synonym: VCM66_1578

Alternate gene names: 227081791

Gene position: 1712043-1713431 (Clockwise)

Preceding gene: 227081790

Following gene: 227081792

Centisome position: 59.19

GC content: 49.39

Gene sequence:

>1389_bases
TTGGGTATCAGTTGCATGCCAATTAAATCTCGGGCGTTACGTCATCTCAGCCTAAAAACCCGCTTGCTCCTTGCTGCGGC
ACTATGGCTGGGCGCGATGATCTTGGCCGCCGGCTATTTGATCCCAAATCTCATCCATCAATATTTGCTGGAAGATGTGC
AAAACCAGTTGCAACTGTCGATGGATGAGATCACCGCCAACTTGGAAGCCAATCCACAAGGACAATTAACCCTCACCACT
CGACTTGCGGATCCCCGTTTTACCCAGCCTTATAGTGGCCTTTACTGGTCAGCCACTTTAGGCGATCAAACGCTTCGCTC
ACGCTCGTTGTGGGATCGCTTTTTGACCGAAGAAACCCATGTTCACGGTACGATTTATCTTGGCGCGAAAGAGGAATCGT
TAATTGTATTAAAGCGTACGGTTTATCTGCCCGAATTTTCACAACCCATCACAATCACGATAGGCTTAGATGATGCCCCG
CTAAAAGCCACGCTACAGCGCGTGAGCAAAGAGTTGTGGATGATTTTGGCTCTATTGTTCAGCGGGATACTCGTTTTAAC
TGGCGTACAAGTGACGTGGTCACTCCGCCCACTTTCGAAAATGCAGCGCGAACTCTCAGCCTTGCGAGATGGCCAGCAAA
ATGCGTTGAATGGCGATTACCCACAAGAGATTTCACCGCTAGTCAATGATCTCAACGCTCTCCTCTTTCATTACCAAGAG
CTGCTTGAGCGAGCCAGACACCATGCGGGGAACTTATCCCACGCATTAAAAACACCACTTTCTGTGCTCAAAAACGAAGT
CACTCAGTTGAATGATGCCCAGCTCAGAATCCAGTTACAGCCTTCTGTTGAGCAGATCCAGCAGCAAATTGACTACCATC
TAGGGCGTGCTCGTATGGCGGGTTCCGCCAATATTCTTTCGGTTAAAACCGCACCCAGTGCGCGAGTCGATGCTATTTCA
CAAGCATTTGATAAGGTTTACGCTGAACGTGGAATCACCTTGGTGAATGAGCTGGATTCGGACTTAGAAGTGGCCGTTGA
ACCGACTGACCTAGATGAAATGATCGGTAACCTTCTGGAAAACAGCTACAAATGGGCCAACAGCTTAATTCGTGTACACA
GCCAAATACTGAGTGACGATTGGGTACAGATCTGCGTGGAAGACGATGGCGCAGGCATCAGTGCAACGAACCGCGAAAAA
GCCCTCAAACGCGGAGTGCGCTTGGATGAAACCACGCCCGGCACTGGGCTTGGGCTTAATATTGTGAGTGAAATGGCGCA
CAGTTATCGTGGAGCATTGGCTTTAGGAGAGAGTCAGTTAGGCGGCTTAAAAGCCACTCTGACCTTAAAACAACCCACTC
GCGCAGCCAAAGCCGTCAAATCCCATTAA

Upstream 100 bases:

>100_bases
TATAAGCAAGATTTTGACCGCGATTCCAATACCATTGAGGTGTTCATCGGACGCATTCGTAAGAAAATTGCGCCGGATGT
GATCAAAACTGTGCGCGGTC

Downstream 100 bases:

>100_bases
ATGCGGTGCAAAAACGGATTTTCAGTTGCCCGAGAGAGCAATGAATGCTAGTATCCGCGCTCGTTTCTGACCCTCCTATA
GGTTTGGAAACTCTACGACA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MGISCMPIKSRALRHLSLKTRLLLAAALWLGAMILAAGYLIPNLIHQYLLEDVQNQLQLSMDEITANLEANPQGQLTLTT
RLADPRFTQPYSGLYWSATLGDQTLRSRSLWDRFLTEETHVHGTIYLGAKEESLIVLKRTVYLPEFSQPITITIGLDDAP
LKATLQRVSKELWMILALLFSGILVLTGVQVTWSLRPLSKMQRELSALRDGQQNALNGDYPQEISPLVNDLNALLFHYQE
LLERARHHAGNLSHALKTPLSVLKNEVTQLNDAQLRIQLQPSVEQIQQQIDYHLGRARMAGSANILSVKTAPSARVDAIS
QAFDKVYAERGITLVNELDSDLEVAVEPTDLDEMIGNLLENSYKWANSLIRVHSQILSDDWVQICVEDDGAGISATNREK
ALKRGVRLDETTPGTGLGLNIVSEMAHSYRGALALGESQLGGLKATLTLKQPTRAAKAVKSH

Sequences:

>Translated_462_residues
MGISCMPIKSRALRHLSLKTRLLLAAALWLGAMILAAGYLIPNLIHQYLLEDVQNQLQLSMDEITANLEANPQGQLTLTT
RLADPRFTQPYSGLYWSATLGDQTLRSRSLWDRFLTEETHVHGTIYLGAKEESLIVLKRTVYLPEFSQPITITIGLDDAP
LKATLQRVSKELWMILALLFSGILVLTGVQVTWSLRPLSKMQRELSALRDGQQNALNGDYPQEISPLVNDLNALLFHYQE
LLERARHHAGNLSHALKTPLSVLKNEVTQLNDAQLRIQLQPSVEQIQQQIDYHLGRARMAGSANILSVKTAPSARVDAIS
QAFDKVYAERGITLVNELDSDLEVAVEPTDLDEMIGNLLENSYKWANSLIRVHSQILSDDWVQICVEDDGAGISATNREK
ALKRGVRLDETTPGTGLGLNIVSEMAHSYRGALALGESQLGGLKATLTLKQPTRAAKAVKSH
>Mature_461_residues
GISCMPIKSRALRHLSLKTRLLLAAALWLGAMILAAGYLIPNLIHQYLLEDVQNQLQLSMDEITANLEANPQGQLTLTTR
LADPRFTQPYSGLYWSATLGDQTLRSRSLWDRFLTEETHVHGTIYLGAKEESLIVLKRTVYLPEFSQPITITIGLDDAPL
KATLQRVSKELWMILALLFSGILVLTGVQVTWSLRPLSKMQRELSALRDGQQNALNGDYPQEISPLVNDLNALLFHYQEL
LERARHHAGNLSHALKTPLSVLKNEVTQLNDAQLRIQLQPSVEQIQQQIDYHLGRARMAGSANILSVKTAPSARVDAISQ
AFDKVYAERGITLVNELDSDLEVAVEPTDLDEMIGNLLENSYKWANSLIRVHSQILSDDWVQICVEDDGAGISATNREKA
LKRGVRLDETTPGTGLGLNIVSEMAHSYRGALALGESQLGGLKATLTLKQPTRAAKAVKSH

Specific function: Member of the two-component regulatory system phoQ/phoP which regulates the expression of genes involved in virulence and resistance to host defense antimicrobial peptides. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=293, Percent_Identity=32.0819112627986, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790551, Length=276, Percent_Identity=27.536231884058, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=225, Percent_Identity=29.7777777777778, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788393, Length=264, Percent_Identity=25.3787878787879, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=312, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789403, Length=292, Percent_Identity=25.6849315068493, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787894, Length=236, Percent_Identity=27.1186440677966, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51185; Mature: 51054

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISCMPIKSRALRHLSLKTRLLLAAALWLGAMILAAGYLIPNLIHQYLLEDVQNQLQLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDEITANLEANPQGQLTLTTRLADPRFTQPYSGLYWSATLGDQTLRSRSLWDRFLTEETH
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCC
VHGTIYLGAKEESLIVLKRTVYLPEFSQPITITIGLDDAPLKATLQRVSKELWMILALLF
EEEEEEECCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
SGILVLTGVQVTWSLRPLSKMQRELSALRDGQQNALNGDYPQEISPLVNDLNALLFHYQE
HHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLERARHHAGNLSHALKTPLSVLKNEVTQLNDAQLRIQLQPSVEQIQQQIDYHLGRARMA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
GSANILSVKTAPSARVDAISQAFDKVYAERGITLVNELDSDLEVAVEPTDLDEMIGNLLE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECCCCHHHHHHHHHH
NSYKWANSLIRVHSQILSDDWVQICVEDDGAGISATNREKALKRGVRLDETTPGTGLGLN
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHH
IVSEMAHSYRGALALGESQLGGLKATLTLKQPTRAAKAVKSH
HHHHHHHHHCCCCEECCCCCCCEEEEEEECCCHHHHHHHCCC
>Mature Secondary Structure 
GISCMPIKSRALRHLSLKTRLLLAAALWLGAMILAAGYLIPNLIHQYLLEDVQNQLQLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDEITANLEANPQGQLTLTTRLADPRFTQPYSGLYWSATLGDQTLRSRSLWDRFLTEETH
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCC
VHGTIYLGAKEESLIVLKRTVYLPEFSQPITITIGLDDAPLKATLQRVSKELWMILALLF
EEEEEEECCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
SGILVLTGVQVTWSLRPLSKMQRELSALRDGQQNALNGDYPQEISPLVNDLNALLFHYQE
HHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLERARHHAGNLSHALKTPLSVLKNEVTQLNDAQLRIQLQPSVEQIQQQIDYHLGRARMA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
GSANILSVKTAPSARVDAISQAFDKVYAERGITLVNELDSDLEVAVEPTDLDEMIGNLLE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECCCCHHHHHHHHHH
NSYKWANSLIRVHSQILSDDWVQICVEDDGAGISATNREKALKRGVRLDETTPGTGLGLN
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHH
IVSEMAHSYRGALALGESQLGGLKATLTLKQPTRAAKAVKSH
HHHHHHHHHCCCCEECCCCCCCEEEEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA