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The map label for this gene is bcr [H]

Identifier: 227081786

GI number: 227081786

Start: 1706588

End: 1707793

Strand: Reverse

Name: bcr [H]

Synonym: VCM66_1573

Alternate gene names: 227081786

Gene position: 1707793-1706588 (Counterclockwise)

Preceding gene: 227081787

Following gene: 227081785

Centisome position: 59.04

GC content: 51.58

Gene sequence:

>1206_bases
ATGTCTGCCTCAACCGTGAATACCCAACAAACACCGCAAATGAGTTTGTTGCTTTATATTGTGCTCGGTGCGCTCGGTGC
GTTGACACCGCTGGCGATCGATATGTATTTGCCAGCCATGCCGACCATTGCCCGTGATTTCGGCGTTAACGCTGGTGCGG
TGCAAATTACTCTAACTGCCTATACGGCAGGCTTTGCCATTGGCCAATTGCTGCATGGTCCGCTGGCCGATAGCTTTGGT
CGCCGTCCGGTGATGCTGTGCGGTATCTTCCTCTTTGGTATTGCCGCTATGGTGAGCGCGACGACCAATGGTATTGAAGC
CTTAACCTATATTCGTGCCGCGCAAGGTTTTGCGGGTGCGGCGGCGGCCGTGGTCATTCAAGCGGTGGTACGCGATATGT
TTGACCGCGAAGATTTTGCGCGGGCGATGTCGTTTGTCACTTTAGTGGTGACCTTGGCTCCTTTGGTTGCGCCTATGATT
GGCGGGCACTTAGCGATTTGGTTTGGCTGGCGTTCTATCTTCTGGGTGCTGGCGCTGTTTGCGGCACTCGTTATCGCGAT
GGTGATGTGGAAAATCCCCGAAACGCTGAGCCCTGAAAATCGTCAGCCGCTGCGTTTTCGTAGCACCATGCGCAACTATT
TTGATTTGTGTAAAAACCCTGTAGCTATGGGGCTGATTTTCTCTGGCGCATTTTCGTTTGCGGGTATGTTCGCGTTTTTG
ACAGCCGGCGCGTTTGTGTACATCGATATTTATGGTGTTAGCCCAGACGGTTTCGGATACCTGTTTGGCCTTAATATTGT
CGCGATGATTGCGCTGACCACGATCAACGGCCGTTTTGTCAAAAAAATCGGCTCACACAACATGCTACGCTTCGCACTCT
TTTTACAGTTGATGGCGGGGATTGGTCTGTTTGTTAGCTGGGTTCTGGATTGGGGATTATGGGGAACCGTGCCATTCGTG
GTGTTGTTTGTCGGTACAATCTCAACCATTGGCAGTAACTCGATGGGGTTGTTGCTGAGTAAATACCCGCGTATGGCGGG
AACCGCATCATCATTAGCGGGCACATTGCGTTTTGGTACGGGCTCAATCATTGGCGCTGTCGTGGCAGCGTTGCCGGGTA
CTGAAACATGGCCAATGATATTTGTGATGGCGGCTTGTTCTGTGTTATCAGGTGCCTTTTACTGGACGTTAGGAAGAAAA
GCGTAA

Upstream 100 bases:

>100_bases
CGTTAAGTCCTCCCACGTTTTATTGCCTCATCAAGCAATCTCTAATTCGCATACACTTATGGGTGTACGCGAAAAACTCA
ATTTAACTCTACGGAGAAGT

Downstream 100 bases:

>100_bases
TGGCGAATTACCATTTAGAAATTCAAAATGTGGTCAATACTGCACTGGCAGAATTAGAGGCCGAACATAAAGCGGGCAAG
CTTGCCAATGCGCCAGTGGC

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSASTVNTQQTPQMSLLLYIVLGALGALTPLAIDMYLPAMPTIARDFGVNAGAVQITLTAYTAGFAIGQLLHGPLADSFG
RRPVMLCGIFLFGIAAMVSATTNGIEALTYIRAAQGFAGAAAAVVIQAVVRDMFDREDFARAMSFVTLVVTLAPLVAPMI
GGHLAIWFGWRSIFWVLALFAALVIAMVMWKIPETLSPENRQPLRFRSTMRNYFDLCKNPVAMGLIFSGAFSFAGMFAFL
TAGAFVYIDIYGVSPDGFGYLFGLNIVAMIALTTINGRFVKKIGSHNMLRFALFLQLMAGIGLFVSWVLDWGLWGTVPFV
VLFVGTISTIGSNSMGLLLSKYPRMAGTASSLAGTLRFGTGSIIGAVVAALPGTETWPMIFVMAACSVLSGAFYWTLGRK
A

Sequences:

>Translated_401_residues
MSASTVNTQQTPQMSLLLYIVLGALGALTPLAIDMYLPAMPTIARDFGVNAGAVQITLTAYTAGFAIGQLLHGPLADSFG
RRPVMLCGIFLFGIAAMVSATTNGIEALTYIRAAQGFAGAAAAVVIQAVVRDMFDREDFARAMSFVTLVVTLAPLVAPMI
GGHLAIWFGWRSIFWVLALFAALVIAMVMWKIPETLSPENRQPLRFRSTMRNYFDLCKNPVAMGLIFSGAFSFAGMFAFL
TAGAFVYIDIYGVSPDGFGYLFGLNIVAMIALTTINGRFVKKIGSHNMLRFALFLQLMAGIGLFVSWVLDWGLWGTVPFV
VLFVGTISTIGSNSMGLLLSKYPRMAGTASSLAGTLRFGTGSIIGAVVAALPGTETWPMIFVMAACSVLSGAFYWTLGRK
A
>Mature_400_residues
SASTVNTQQTPQMSLLLYIVLGALGALTPLAIDMYLPAMPTIARDFGVNAGAVQITLTAYTAGFAIGQLLHGPLADSFGR
RPVMLCGIFLFGIAAMVSATTNGIEALTYIRAAQGFAGAAAAVVIQAVVRDMFDREDFARAMSFVTLVVTLAPLVAPMIG
GHLAIWFGWRSIFWVLALFAALVIAMVMWKIPETLSPENRQPLRFRSTMRNYFDLCKNPVAMGLIFSGAFSFAGMFAFLT
AGAFVYIDIYGVSPDGFGYLFGLNIVAMIALTTINGRFVKKIGSHNMLRFALFLQLMAGIGLFVSWVLDWGLWGTVPFVV
LFVGTISTIGSNSMGLLLSKYPRMAGTASSLAGTLRFGTGSIIGAVVAALPGTETWPMIFVMAACSVLSGAFYWTLGRKA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=385, Percent_Identity=47.7922077922078, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI87082312, Length=355, Percent_Identity=28.7323943661972, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI48994889, Length=238, Percent_Identity=33.1932773109244, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790146, Length=373, Percent_Identity=26.8096514745308, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790794, Length=288, Percent_Identity=25, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787065, Length=186, Percent_Identity=28.494623655914, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787880, Length=206, Percent_Identity=27.6699029126214, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081738, Length=175, Percent_Identity=26.8571428571429, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790195, Length=138, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081983, Length=174, Percent_Identity=24.7126436781609, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=212, Percent_Identity=25, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=170, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=160, Percent_Identity=28.125, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325414, Length=254, Percent_Identity=23.2283464566929, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321838, Length=176, Percent_Identity=26.1363636363636, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321577, Length=254, Percent_Identity=23.2283464566929, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42916; Mature: 42784

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHEECCCCC
>Mature Secondary Structure 
SASTVNTQQTPQMSLLLYIVLGALGALTPLAIDMYLPAMPTIARDFGVNAGAVQITLTA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEH
YTAGFAIGQLLHGPLADSFGRRPVMLCGIFLFGIAAMVSATTNGIEALTYIRAAQGFAGA
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
AAAVVIQAVVRDMFDREDFARAMSFVTLVVTLAPLVAPMIGGHLAIWFGWRSIFWVLALF
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHH
AALVIAMVMWKIPETLSPENRQPLRFRSTMRNYFDLCKNPVAMGLIFSGAFSFAGMFAFL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TAGAFVYIDIYGVSPDGFGYLFGLNIVAMIALTTINGRFVKKIGSHNMLRFALFLQLMAG
HCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHH
IGLFVSWVLDWGLWGTVPFVVLFVGTISTIGSNSMGLLLSKYPRMAGTASSLAGTLRFGT
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHCCC
GSIIGAVVAALPGTETWPMIFVMAACSVLSGAFYWTLGRKA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]