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The map label for this gene is rsuA

Identifier: 227081787

GI number: 227081787

Start: 1708112

End: 1708807

Strand: Reverse

Name: rsuA

Synonym: VCM66_1574

Alternate gene names: 227081787

Gene position: 1708807-1708112 (Counterclockwise)

Preceding gene: 227081793

Following gene: 227081786

Centisome position: 59.08

GC content: 47.99

Gene sequence:

>696_bases
ATGCGTTTAGACAAATTCCTGTGTGATGCGCTAGGTACAACGCGCAAAGAGGCCACTCAACTTCTCAAAAGTGGTGAAGT
GACCGTCGATGATGTGGTGCAAAAAAGTGGTGCATTCAAACTCAAAGAGACGTCGTGCGTTGAGTGGCAAGGCCGTGAAA
TTACGTTGCATGGTCCGCGTTACATCATGATGCACAAACCGGATGGTGTGGTCTGCTCGCATGAGGATGGTTTTAACCAA
ACGGCGCTTACCTTGCTTGATGAAGTTAATATTCAAGATCTGCACTTTGCAGGCCGCTTGGATGTCGATACCACAGGTTT
ACTACTGATCACTGATGATGGGCAATGGTCGCACCGAGTCACGTCACCAAAACACAAGTGCGAGAAGACCTACCGCGTGT
GGTTGGCTGATCCCATTCAAGCTGATTACGCAGAGCAATTTAACCGAGGTATTGAATTGCGTGGTGAACGTGAGCTGACT
TTGCCCGCGCAACTGGAGGTGATCAGTGAAACGGAAGTGCTATTGACCATCCATGAAGGCAAGTATCACCAAGTGAAGCG
TATGTTTGCCGCTTTAGGTAACAAAGTCATAGGGTTACATCGTGAGCGCATTGGTCAGATTACCTTAGATGAAACCCTTG
AACCGGGGGAATACCGCTATTTGACGGAAGAAGAAGTGGCCTCAATCTGGAAATAA

Upstream 100 bases:

>100_bases
GACGGCTTTTGCATCGTTTGATGTCGGCCGCAAGCAATTGCGCTGGGTAATTATTGTGTGAGGTCTGTATAATAGCCGAC
TTCCAGCAGATGAGGTAAGC

Downstream 100 bases:

>100_bases
ACCAAGATTTAGGAAATAGATTGTTTCTGAATTAGCACTAGTTAATCGCGGCGATTATTAAGATCATATACCCAAACAAC
TTGGAGTTGCAGGTAGGCGG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREITLHGPRYIMMHKPDGVVCSHEDGFNQ
TALTLLDEVNIQDLHFAGRLDVDTTGLLLITDDGQWSHRVTSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELT
LPAQLEVISETEVLLTIHEGKYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK

Sequences:

>Translated_231_residues
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREITLHGPRYIMMHKPDGVVCSHEDGFNQ
TALTLLDEVNIQDLHFAGRLDVDTTGLLLITDDGQWSHRVTSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELT
LPAQLEVISETEVLLTIHEGKYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK
>Mature_231_residues
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREITLHGPRYIMMHKPDGVVCSHEDGFNQ
TALTLLDEVNIQDLHFAGRLDVDTTGLLLITDDGQWSHRVTSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELT
LPAQLEVISETEVLLTIHEGKYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=57.3913043478261, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=25.9414225941423, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=27.7056277056277, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=30.6010928961749, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSUA_VIBCH (Q9KRK5)

Other databases:

- EMBL:   AE003852
- PIR:   A82176
- RefSeq:   NP_231272.1
- ProteinModelPortal:   Q9KRK5
- SMR:   Q9KRK5
- GeneID:   2613890
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1635
- TIGR:   VC_1635
- HOGENOM:   HBG754478
- OMA:   SPKRHVP
- ProtClustDB:   CLSK874466
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26362; Mature: 26362

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREITLHGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCEEEECCCEEEEECCE
YIMMHKPDGVVCSHEDGFNQTALTLLDEVNIQDLHFAGRLDVDTTGLLLITDDGQWSHRV
EEEEECCCCEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCC
TSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELTLPAQLEVISETEVLLTIHEG
CCCHHHHHHEEEEEEECCCCHHHHHHHCCCCEECCCCEEECCCEEEEECCCEEEEEEECC
KYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK
CHHHHHHHHHHHCCCEEEEHHHHHCCEEECCCCCCCCEEEECHHHHHHHCC
>Mature Secondary Structure
MRLDKFLCDALGTTRKEATQLLKSGEVTVDDVVQKSGAFKLKETSCVEWQGREITLHGPR
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCEEEECCCEEEEECCE
YIMMHKPDGVVCSHEDGFNQTALTLLDEVNIQDLHFAGRLDVDTTGLLLITDDGQWSHRV
EEEEECCCCEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCC
TSPKHKCEKTYRVWLADPIQADYAEQFNRGIELRGERELTLPAQLEVISETEVLLTIHEG
CCCHHHHHHEEEEEEECCCCHHHHHHHCCCCEECCCCEEECCCEEEEECCCEEEEEEECC
KYHQVKRMFAALGNKVIGLHRERIGQITLDETLEPGEYRYLTEEEVASIWK
CHHHHHHHHHHHCCCEEEEHHHHHCCEEECCCCCCCCEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301