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The map label for this gene is rlmF [H]

Identifier: 227081767

GI number: 227081767

Start: 1675778

End: 1676866

Strand: Reverse

Name: rlmF [H]

Synonym: VCM66_1554

Alternate gene names: 227081767

Gene position: 1676866-1675778 (Counterclockwise)

Preceding gene: 227081768

Following gene: 227081766

Centisome position: 57.97

GC content: 46.74

Gene sequence:

>1089_bases
ATGAACACTCCACTTAAGCCAAAACATGGGCAAAAAACAAATCGAAAACCGAAAGCCAATAAGCCAGTTGTGAAAAAGCA
GCAAACTAAGCAGCCGCCAACCCACAAAGTACAAGGTGAGGAAGTCGCTGCCGTTAAATCTGGATTACATCCACGTAATC
GTCACCGCGGGCAGTATGATTTTCCGGCACTGATTAAGGTCGTGCCAGAGTTGCAATCCCATGTGATGAAAAATCCTAAA
GGGCAATGGACGATTAATTTTGCCGATCCGATCTCGGTCAAGTTACTCAATAAAGCGCTTTTGGTTCTGCATTACGGTGT
CACCTATTGGGATATCCCAGAGGGCTTTTTATGCCCGCCCATTCCGGGTCGTGCGGATTACATTCATCGCGTGGCCGATC
TATTGCTTAAAGGCAATCCCCAATTAAACCACTCTCAAGTCAGAGCACTGGATATTGGCGTGGGCGCAAATTGTATCTAT
CCGATTGTTGGAGTGACGGAATATGGCTGGTCATGGGTTGGCAGTGATGTTGATCCTGTTTCAATTCAACAAGCGAGTTT
GATTGTTCAAAGCAACTCGAAGCTGCAAGGCCATATCGAATGCCGCCTGCAAAAGAACAGCCAACATATTTTTAACGGCA
TTATTGGGGCGAATGAGCGTTATACGGTGACGACTTGCAACCCGCCTTTTCATGCATCCTTAGCGGACGCTCAGCAAGGA
ACCCAGCGTAAGTTGACCAACTTACAAGCAAATCAGCGCAAGAAAGGCCGCTTAGCAACGCCAACGTTAAGCCACTCAAG
GTTAAATTTTGGTGGTCAGAAAGCCGAGTTATGGTGTCCAGGAGGAGAAGCGGCTTTCATTGGTAAAATGGCGGTAGAAA
GCCAGCAGTTCGCGCAGCAAGTGCTTTGGTTTAGCACTTTGATTTCTAAAGGTGATAACGTTCGTGGCATGAAAAAACAG
CTGGAAAAACTCGGGGCTCAGTCGATACATGTAATAGAGATGGCACAAGGGCAGAAGATAAGTCGCTTTATTGCTTGGAG
TTTTCAAAATGCTGAGCAGCGCAAGCTGTGGTGGCAGGCAAAATGTTAA

Upstream 100 bases:

>100_bases
CGTTTTATCAGCTCTGTTGCCATATGGAACCGAATTATCACCAGCTTGAACTGGATGCACGGCTATACATGATGGTGCTA
GAGCTTAAGGCAGGCAAAGC

Downstream 100 bases:

>100_bases
GCTTTAAACAAGTTATACCCTTCCTAGTTAAAGTTGCAGCGGTGTTGGCTACGTTCGTTCACCCCAATCACATAGTTCGC
CTATGCTCATGGGGATTCAC

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNTPLKPKHGQKTNRKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYDFPALIKVVPELQSHVMKNPK
GQWTINFADPISVKLLNKALLVLHYGVTYWDIPEGFLCPPIPGRADYIHRVADLLLKGNPQLNHSQVRALDIGVGANCIY
PIVGVTEYGWSWVGSDVDPVSIQQASLIVQSNSKLQGHIECRLQKNSQHIFNGIIGANERYTVTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIGKMAVESQQFAQQVLWFSTLISKGDNVRGMKKQ
LEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQAKC

Sequences:

>Translated_362_residues
MNTPLKPKHGQKTNRKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYDFPALIKVVPELQSHVMKNPK
GQWTINFADPISVKLLNKALLVLHYGVTYWDIPEGFLCPPIPGRADYIHRVADLLLKGNPQLNHSQVRALDIGVGANCIY
PIVGVTEYGWSWVGSDVDPVSIQQASLIVQSNSKLQGHIECRLQKNSQHIFNGIIGANERYTVTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIGKMAVESQQFAQQVLWFSTLISKGDNVRGMKKQ
LEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQAKC
>Mature_362_residues
MNTPLKPKHGQKTNRKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYDFPALIKVVPELQSHVMKNPK
GQWTINFADPISVKLLNKALLVLHYGVTYWDIPEGFLCPPIPGRADYIHRVADLLLKGNPQLNHSQVRALDIGVGANCIY
PIVGVTEYGWSWVGSDVDPVSIQQASLIVQSNSKLQGHIECRLQKNSQHIFNGIIGANERYTVTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIGKMAVESQQFAQQVLWFSTLISKGDNVRGMKKQ
LEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQAKC

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=310, Percent_Identity=30.3225806451613, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI87081786, Length=314, Percent_Identity=50.6369426751592, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32564931, Length=308, Percent_Identity=23.7012987012987, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564933, Length=308, Percent_Identity=21.4285714285714, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281363415, Length=305, Percent_Identity=28.1967213114754, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922302, Length=305, Percent_Identity=28.1967213114754, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 40486; Mature: 40486

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPLKPKHGQKTNRKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
FPALIKVVPELQSHVMKNPKGQWTINFADPISVKLLNKALLVLHYGVTYWDIPEGFLCPP
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHEECCEEEECCCCCCCCC
IPGRADYIHRVADLLLKGNPQLNHSQVRALDIGVGANCIYPIVGVTEYGWSWVGSDVDPV
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHCCCCHHCCCEECCCCCCCC
SIQQASLIVQSNSKLQGHIECRLQKNSQHIFNGIIGANERYTVTTCNPPFHASLADAQQG
EEEEEEEEEECCCCEEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCC
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIGKMAVESQQFAQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHH
VLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQA
HHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHEEEECCCCHHHCCEEEC
KC
CC
>Mature Secondary Structure
MNTPLKPKHGQKTNRKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
FPALIKVVPELQSHVMKNPKGQWTINFADPISVKLLNKALLVLHYGVTYWDIPEGFLCPP
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHEECCEEEECCCCCCCCC
IPGRADYIHRVADLLLKGNPQLNHSQVRALDIGVGANCIYPIVGVTEYGWSWVGSDVDPV
CCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHCCCCHHCCCEECCCCCCCC
SIQQASLIVQSNSKLQGHIECRLQKNSQHIFNGIIGANERYTVTTCNPPFHASLADAQQG
EEEEEEEEEECCCCEEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCC
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIGKMAVESQQFAQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHH
VLWFSTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQA
HHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHEEEECCCCHHHCCEEEC
KC
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA