Click here to switch to the map view.

The map label for this gene is ycdT [H]

Identifier: 227081746

GI number: 227081746

Start: 1652435

End: 1653724

Strand: Direct

Name: ycdT [H]

Synonym: VCM66_1533

Alternate gene names: 227081746

Gene position: 1652435-1653724 (Clockwise)

Preceding gene: 227081745

Following gene: 227081751

Centisome position: 57.13

GC content: 42.79

Gene sequence:

>1290_bases
ATGATGGACACAGATAACCGCTGGAGAATTGCGCTTCGCTCTATACGAGGAGAACTGATCATTATCGGGATGATAGCGGT
CAGCATAGTATTGATTAACACGCCTAAATTATTGTTTGAAGATAAGATCCACGCTTATGAAAAAGAGCAATTGCAACTGA
CTGTCGAACTACAGAAAACCCGTATTGACTACGCAAGCCTAATTCTAGAACATCTAGAAACCCACTCATTGACTCCACAG
TTGCAAGAATTCTTCAATCTGTACCATGGCAATTTATGTGTGCTGGATGAGAATTCCCCACAACAATGCCCGTTGAGCAT
TATCGATGCGGTTCAATCCACCACTTCAGCAAGTGATTTTGTCGCTTCTACCTCAGTAAACATCACGTTTGCAGAAGGTT
CTATCCTATTTTTTCAAGTGCAAGGCAAACAGATCCGAGTGCTATGGTTCAACCATGCTTCATTTTGGCCAGCACCACAG
AAAAACATCAGCTTGGTCTTGGTTCGCGGTAATCACATTCAGTTCTTAGATAATCCGATTGCACACTATAACGCTCTACT
GAATTACACGATTTTGCGCCACCGAGATGATTACGGGACTTTGCAGTTTGAAAACATGGAGTTGAGCTTTCTTCGTTGGA
ACCTTGGTGAATATCAATTGATCAGTGTGTTTAACGACAAAGTACGCTTGTTTAATCTTATTTACTGGGTAATGTCGGCC
AGTTTAACCCTGTTACTGACTTTGACTTGGTTCCTCACTCGACTCAATCTACAAAAAGTAGCGGCGCAGCGTCATGTTTA
TATCGACAACTTAACTCGGCTCAATAACCGACATTTTCTGAATAAAATCAGTACTAAGTTTATTGAACACCCTCACTCCA
TTGCCGCGATGATCGACATCGATCACTTTAAGAAAATTAATGACCAATATGGGCACATTACCGGAGATCGCATTCTACAG
GCGGTGGCCTCTTGTCTGCGCAAAAATGTCCGAGATGGAGACACCATTATCCGCTTTGGTGGAGAAGAGTTTTTACTATT
GTTCCAAGCCCATTCAAACAAAGAAGCTTGGTACATGCTCGACCGATTGCGTCAGCGAGTTAAAGACGATCATAGCTTGT
ACGGAACAACGATTTCGATTGGTTTTACTTTTATCGATGGTAGTTTACCCGCTGCTATTTCGCGGGCAGATACTGCACTC
TATCAAGCAAAAGAAGCAGGACGTAATCAAGTGGTTGCCGACAGCGAGAAAGAGAATGACTCAGCAGCAGATTTACCGAT
TCAGTGCTGA

Upstream 100 bases:

>100_bases
CGTATGTCTGCGTTATAGCCTCAAGTTATCTCCTGCTTACTTGCTGGGAAACCAAGGATTGGGATTAGCGTATATACACC
GGATGTGATGAATAAAAAGA

Downstream 100 bases:

>100_bases
GTCTTATGCCCAAGATGTTAAAAAACAAACTGATAGCGCGTTTGATAGCGATAGACTTCTCCGGGTTGCAGGATGCAGCT
CGGTTGCAGCCATTCTGGAT

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MMDTDNRWRIALRSIRGELIIIGMIAVSIVLINTPKLLFEDKIHAYEKEQLQLTVELQKTRIDYASLILEHLETHSLTPQ
LQEFFNLYHGNLCVLDENSPQQCPLSIIDAVQSTTSASDFVASTSVNITFAEGSILFFQVQGKQIRVLWFNHASFWPAPQ
KNISLVLVRGNHIQFLDNPIAHYNALLNYTILRHRDDYGTLQFENMELSFLRWNLGEYQLISVFNDKVRLFNLIYWVMSA
SLTLLLTLTWFLTRLNLQKVAAQRHVYIDNLTRLNNRHFLNKISTKFIEHPHSIAAMIDIDHFKKINDQYGHITGDRILQ
AVASCLRKNVRDGDTIIRFGGEEFLLLFQAHSNKEAWYMLDRLRQRVKDDHSLYGTTISIGFTFIDGSLPAAISRADTAL
YQAKEAGRNQVVADSEKENDSAADLPIQC

Sequences:

>Translated_429_residues
MMDTDNRWRIALRSIRGELIIIGMIAVSIVLINTPKLLFEDKIHAYEKEQLQLTVELQKTRIDYASLILEHLETHSLTPQ
LQEFFNLYHGNLCVLDENSPQQCPLSIIDAVQSTTSASDFVASTSVNITFAEGSILFFQVQGKQIRVLWFNHASFWPAPQ
KNISLVLVRGNHIQFLDNPIAHYNALLNYTILRHRDDYGTLQFENMELSFLRWNLGEYQLISVFNDKVRLFNLIYWVMSA
SLTLLLTLTWFLTRLNLQKVAAQRHVYIDNLTRLNNRHFLNKISTKFIEHPHSIAAMIDIDHFKKINDQYGHITGDRILQ
AVASCLRKNVRDGDTIIRFGGEEFLLLFQAHSNKEAWYMLDRLRQRVKDDHSLYGTTISIGFTFIDGSLPAAISRADTAL
YQAKEAGRNQVVADSEKENDSAADLPIQC
>Mature_429_residues
MMDTDNRWRIALRSIRGELIIIGMIAVSIVLINTPKLLFEDKIHAYEKEQLQLTVELQKTRIDYASLILEHLETHSLTPQ
LQEFFNLYHGNLCVLDENSPQQCPLSIIDAVQSTTSASDFVASTSVNITFAEGSILFFQVQGKQIRVLWFNHASFWPAPQ
KNISLVLVRGNHIQFLDNPIAHYNALLNYTILRHRDDYGTLQFENMELSFLRWNLGEYQLISVFNDKVRLFNLIYWVMSA
SLTLLLTLTWFLTRLNLQKVAAQRHVYIDNLTRLNNRHFLNKISTKFIEHPHSIAAMIDIDHFKKINDQYGHITGDRILQ
AVASCLRKNVRDGDTIIRFGGEEFLLLFQAHSNKEAWYMLDRLRQRVKDDHSLYGTTISIGFTFIDGSLPAAISRADTAL
YQAKEAGRNQVVADSEKENDSAADLPIQC

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=191, Percent_Identity=34.5549738219895, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=37.6543209876543, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=198, Percent_Identity=31.8181818181818, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=124, Percent_Identity=37.0967741935484, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=37.6470588235294, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=156, Percent_Identity=32.6923076923077, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=158, Percent_Identity=31.0126582278481, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=32.1637426900585, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=32.2368421052632, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=152, Percent_Identity=32.2368421052632, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786584, Length=121, Percent_Identity=33.8842975206612, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787056, Length=167, Percent_Identity=26.9461077844311, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49328; Mature: 49328

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDTDNRWRIALRSIRGELIIIGMIAVSIVLINTPKLLFEDKIHAYEKEQLQLTVELQKT
CCCCCCHHHHHHHHHCCCEEEEEEHHHEEEEECCCHHHHHHHHHHCCCCEEEEEEEEEHH
RIDYASLILEHLETHSLTPQLQEFFNLYHGNLCVLDENSPQQCPLSIIDAVQSTTSASDF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHH
VASTSVNITFAEGSILFFQVQGKQIRVLWFNHASFWPAPQKNISLVLVRGNHIQFLDNPI
EEECEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCCCCCEEEEEEECCEEEECCCCH
AHYNALLNYTILRHRDDYGTLQFENMELSFLRWNLGEYQLISVFNDKVRLFNLIYWVMSA
HHHHHHHHEEEEEECCCCCCEEECCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHH
SLTLLLTLTWFLTRLNLQKVAAQRHVYIDNLTRLNNRHFLNKISTKFIEHPHSIAAMIDI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEH
DHFKKINDQYGHITGDRILQAVASCLRKNVRDGDTIIRFGGEEFLLLFQAHSNKEAWYML
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCHHHHH
DRLRQRVKDDHSLYGTTISIGFTFIDGSLPAAISRADTALYQAKEAGRNQVVADSEKEND
HHHHHHHCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCC
SAADLPIQC
CCCCCCCCC
>Mature Secondary Structure
MMDTDNRWRIALRSIRGELIIIGMIAVSIVLINTPKLLFEDKIHAYEKEQLQLTVELQKT
CCCCCCHHHHHHHHHCCCEEEEEEHHHEEEEECCCHHHHHHHHHHCCCCEEEEEEEEEHH
RIDYASLILEHLETHSLTPQLQEFFNLYHGNLCVLDENSPQQCPLSIIDAVQSTTSASDF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCHHH
VASTSVNITFAEGSILFFQVQGKQIRVLWFNHASFWPAPQKNISLVLVRGNHIQFLDNPI
EEECEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCCCCCEEEEEEECCEEEECCCCH
AHYNALLNYTILRHRDDYGTLQFENMELSFLRWNLGEYQLISVFNDKVRLFNLIYWVMSA
HHHHHHHHEEEEEECCCCCCEEECCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHH
SLTLLLTLTWFLTRLNLQKVAAQRHVYIDNLTRLNNRHFLNKISTKFIEHPHSIAAMIDI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEH
DHFKKINDQYGHITGDRILQAVASCLRKNVRDGDTIIRFGGEEFLLLFQAHSNKEAWYML
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCHHHHH
DRLRQRVKDDHSLYGTTISIGFTFIDGSLPAAISRADTALYQAKEAGRNQVVADSEKEND
HHHHHHHCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCC
SAADLPIQC
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]