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The map label for this gene is yfgF [H]

Identifier: 227081745

GI number: 227081745

Start: 1651498

End: 1652352

Strand: Direct

Name: yfgF [H]

Synonym: VCM66_1532

Alternate gene names: 227081745

Gene position: 1651498-1652352 (Clockwise)

Preceding gene: 227081744

Following gene: 227081746

Centisome position: 57.1

GC content: 45.73

Gene sequence:

>855_bases
ATGAATCACATCCACCCCTACCAAAATGAATTGAGTGATATTTTACGCAAACAGCGCATTACCCCGTTATTCCAACCCAT
TGTGCACCTTGCGAGCGAGCGTGCTATAGGGTACGAGTCGCTCAGTCGAGGCCCTCAACAAAGTCCTCTTCATACGGCGG
GTTCTCTGTTTGAAGCGGCTGAACAGTGCCAGCGCTTACCCGAGTTAGAGAAAATTTGTGTACACAAGGCGGCGGCAGCT
TGGTCAAGCTTCGGGATCGATTCACAGCTTTTTATCAACCTGAGCCCAGATATGTTGCTGCATTCACAACAACACCAGTG
GGAATTACCACAATTGATGCAGCGTCACCGTCTATCCGCCAATCAGGTCGTGTTTGAAATTTCTGAGCGCACCCCTGCAT
TGCAGTTGGATGAGTTGAAAAACTCGGTTCAACATCTACAAGCACAAGGATTTTCAATAGCGATTGATGATATGGGCTCT
GGGTATTCAGGATTAAAACTGTGGTCTGAATTACAGCCGGATTTTGTGAAAATTGATCGCTACTTTATCCATAACATTGA
CAGTAACCCAATCAAACTGAAATTTGTGCGTAGTTTGGTACAACTGGCAGAACAACTTGATTGCCAATTAATTGCAGAGG
GAGTAGAAACGCGCTCCGAACTTTTGGCCATAGCGGATTTAGGTATCGAGTTAGTCCAAGGGTATTTATTCGGTCGCCCT
CAACTTACGCCTCAGCGAATATATCATTCGCAGAGTTTTTCCTCTGCCACGGTCTTAAACATCGAGTCAGCTCGTACAGA
AACGCCTCTTCAGTACCTAATGTTTCAAGATACTCCACGTATGTCTGCGTTATAG

Upstream 100 bases:

>100_bases
TGGATCCTTTACCGCAAAAAATCGGCCATTAATTAATAAAAATGTCACAACAACCGACTAGTGTTTGAGCCGTTTAATGC
ATAAGTATTAGTGGACAACC

Downstream 100 bases:

>100_bases
CCTCAAGTTATCTCCTGCTTACTTGCTGGGAAACCAAGGATTGGGATTAGCGTATATACACCGGATGTGATGAATAAAAA
GAATGATGGACACAGATAAC

Product: EAL family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNHIHPYQNELSDILRKQRITPLFQPIVHLASERAIGYESLSRGPQQSPLHTAGSLFEAAEQCQRLPELEKICVHKAAAA
WSSFGIDSQLFINLSPDMLLHSQQHQWELPQLMQRHRLSANQVVFEISERTPALQLDELKNSVQHLQAQGFSIAIDDMGS
GYSGLKLWSELQPDFVKIDRYFIHNIDSNPIKLKFVRSLVQLAEQLDCQLIAEGVETRSELLAIADLGIELVQGYLFGRP
QLTPQRIYHSQSFSSATVLNIESARTETPLQYLMFQDTPRMSAL

Sequences:

>Translated_284_residues
MNHIHPYQNELSDILRKQRITPLFQPIVHLASERAIGYESLSRGPQQSPLHTAGSLFEAAEQCQRLPELEKICVHKAAAA
WSSFGIDSQLFINLSPDMLLHSQQHQWELPQLMQRHRLSANQVVFEISERTPALQLDELKNSVQHLQAQGFSIAIDDMGS
GYSGLKLWSELQPDFVKIDRYFIHNIDSNPIKLKFVRSLVQLAEQLDCQLIAEGVETRSELLAIADLGIELVQGYLFGRP
QLTPQRIYHSQSFSSATVLNIESARTETPLQYLMFQDTPRMSAL
>Mature_284_residues
MNHIHPYQNELSDILRKQRITPLFQPIVHLASERAIGYESLSRGPQQSPLHTAGSLFEAAEQCQRLPELEKICVHKAAAA
WSSFGIDSQLFINLSPDMLLHSQQHQWELPQLMQRHRLSANQVVFEISERTPALQLDELKNSVQHLQAQGFSIAIDDMGS
GYSGLKLWSELQPDFVKIDRYFIHNIDSNPIKLKFVRSLVQLAEQLDCQLIAEGVETRSELLAIADLGIELVQGYLFGRP
QLTPQRIYHSQSFSSATVLNIESARTETPLQYLMFQDTPRMSAL

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=150, Percent_Identity=36.6666666666667, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=34.6666666666667, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787410, Length=226, Percent_Identity=28.3185840707965, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790496, Length=242, Percent_Identity=25.6198347107438, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788502, Length=236, Percent_Identity=28.3898305084746, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI226510982, Length=245, Percent_Identity=27.7551020408163, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081845, Length=238, Percent_Identity=29.4117647058824, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787055, Length=232, Percent_Identity=29.3103448275862, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=32.1428571428571, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081980, Length=223, Percent_Identity=27.8026905829596, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87081921, Length=237, Percent_Identity=24.4725738396624, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081743, Length=243, Percent_Identity=24.2798353909465, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786507, Length=119, Percent_Identity=30.2521008403361, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 32271; Mature: 32271

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIHPYQNELSDILRKQRITPLFQPIVHLASERAIGYESLSRGPQQSPLHTAGSLFEAA
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHH
EQCQRLPELEKICVHKAAAAWSSFGIDSQLFINLSPDMLLHSQQHQWELPQLMQRHRLSA
HHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHCCCH
NQVVFEISERTPALQLDELKNSVQHLQAQGFSIAIDDMGSGYSGLKLWSELQPDFVKIDR
HHEEEEECCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHH
YFIHNIDSNPIKLKFVRSLVQLAEQLDCQLIAEGVETRSELLAIADLGIELVQGYLFGRP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QLTPQRIYHSQSFSSATVLNIESARTETPLQYLMFQDTPRMSAL
CCCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNHIHPYQNELSDILRKQRITPLFQPIVHLASERAIGYESLSRGPQQSPLHTAGSLFEAA
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHH
EQCQRLPELEKICVHKAAAAWSSFGIDSQLFINLSPDMLLHSQQHQWELPQLMQRHRLSA
HHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHCCCH
NQVVFEISERTPALQLDELKNSVQHLQAQGFSIAIDDMGSGYSGLKLWSELQPDFVKIDR
HHEEEEECCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHH
YFIHNIDSNPIKLKFVRSLVQLAEQLDCQLIAEGVETRSELLAIADLGIELVQGYLFGRP
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QLTPQRIYHSQSFSSATVLNIESARTETPLQYLMFQDTPRMSAL
CCCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]