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The map label for this gene is fumC [H]

Identifier: 227081727

GI number: 227081727

Start: 1632325

End: 1633710

Strand: Direct

Name: fumC [H]

Synonym: VCM66_1513

Alternate gene names: 227081727

Gene position: 1632325-1633710 (Clockwise)

Preceding gene: 227081726

Following gene: 227081734

Centisome position: 56.43

GC content: 51.44

Gene sequence:

>1386_bases
ATGACTCGCGAATACCGAATTGAAAAAGACAGCATGGGTGAGGTCAAAGTCCCTGCTGATGCGCTTTATCAAGCACAAAC
TCAACGTGCCGTAGATAACTTTACGTTAAGCCGCTACACCATGCCTAGCGCGTTTATTGCTGCCCTCGCCTACATTAAAC
AAGCGGCGGCGATGACCAATGCGCAGTTAGGTCATTTAGAAGGGGACATCGCCCAAGCGATTGCCAATGCATCGCAAGCG
GTGATTGATGGCCAGTATGCAAAACAGTTCCCGATTGATGTTTTCCAGACAGGTTCAGGCACCAGCTCCAATATGAACGC
CAATGAGGTGATTGCCACCCTCGCCTCGCGTGAATTGGGCTATGCGGTGAGTCCAAACGATCATGTCAACATGGGACAAA
GCAGTAATGATGTGATCCCAACAGCAATCCAAGTCAGTGCGGCCATCAATATTGAGCAGCAACTGCTCCCCGCCTTGGCG
CATTTAAGTCAAACTCTCGCGGATAAACAGTCAGAGCTGGCTGATGTGGTCAAAACTGGCCGCACCCATTTGATGGATGC
GATGCCCGTGACTTTCGCACAAGAACTGGGTGGATGGAAAAGCCAAATCGACCACGCCGCGCAAGGCATCAGCCATGCGC
TTTCCCCCATCAAAGCCTTGGCGCAAGGCGGCACAGCGGTCGGCACAGGCATTAATGCGGATCCGCGTTTTGCACGTATT
TTTGCCGATAACCTCAGTCAATTGAGTCATGTTGCTTTTCAGCCCAGCGACAATTTTTTCTATAACCTAAGCAGCCAAGA
CTGCGTGGTCGGCCTTTCTGGTCAGTTGAAAACCGCCGCGGTCGCGCTGATGAAAATCGCTAACGATTTGCGCTGGATGA
ACTCTGGCCCTTTGGCGGGGTTAGGGGAAATTGAGCTGCAAGGCTTACAACCGGGCTCCTCGATCATGCCGGGAAAAGTT
AACCCTGTGATCCCGGAAGCCGTGACTATGGTCGCTGCGCAAGTGATTGGCAATGACACTACCATTACCGTGGCTGGCCA
ATCCGGTAATTTCCAGCTTAATGTGATGCTGCCTGTGATCGCGCACAACCTACTGGAATCAATAGAATTATTGACAAATG
CCTGCCATGCGCTCGCCGATAAAGCCATCGCAACCTTTAAAGTTCGTCATGACAATCTGGAAAAAGCCTTGGCTAAAAAC
CCGATTCTCGTCACCGCCTTGAATCCTGTCATTGGTTATCTCAAAGCGGCTGATATTGCCAAAAAAGCCTACCAGCAAGG
TCGCGCGATTTTGGATGTCGCTGCGGAAGAGACGGACCTTGATCGCGCCACGCTTGAAGCGCTGCTTGACCCGAAAAAGC
TCACACAAGGTGGGATTGCCCATTAA

Upstream 100 bases:

>100_bases
ATGTTATCAACAAGTGGATAACGGTGAACTGCGTTTACGCCAACTCAAACAACAATTACAACGAATTCAAGCCCCGCTTG
ATTAACCAAAAGGATCTGTT

Downstream 100 bases:

>100_bases
ACCTTAGCCATAAAACCCTAGTCATAAAACCTTAGCCATAAAACCTTAGCTATAAAAGAATGCGCCCCTCATTGGAGGGG
CGAATTCTAGATATCATTAC

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C 1 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTREYRIEKDSMGEVKVPADALYQAQTQRAVDNFTLSRYTMPSAFIAALAYIKQAAAMTNAQLGHLEGDIAQAIANASQA
VIDGQYAKQFPIDVFQTGSGTSSNMNANEVIATLASRELGYAVSPNDHVNMGQSSNDVIPTAIQVSAAINIEQQLLPALA
HLSQTLADKQSELADVVKTGRTHLMDAMPVTFAQELGGWKSQIDHAAQGISHALSPIKALAQGGTAVGTGINADPRFARI
FADNLSQLSHVAFQPSDNFFYNLSSQDCVVGLSGQLKTAAVALMKIANDLRWMNSGPLAGLGEIELQGLQPGSSIMPGKV
NPVIPEAVTMVAAQVIGNDTTITVAGQSGNFQLNVMLPVIAHNLLESIELLTNACHALADKAIATFKVRHDNLEKALAKN
PILVTALNPVIGYLKAADIAKKAYQQGRAILDVAAEETDLDRATLEALLDPKKLTQGGIAH

Sequences:

>Translated_461_residues
MTREYRIEKDSMGEVKVPADALYQAQTQRAVDNFTLSRYTMPSAFIAALAYIKQAAAMTNAQLGHLEGDIAQAIANASQA
VIDGQYAKQFPIDVFQTGSGTSSNMNANEVIATLASRELGYAVSPNDHVNMGQSSNDVIPTAIQVSAAINIEQQLLPALA
HLSQTLADKQSELADVVKTGRTHLMDAMPVTFAQELGGWKSQIDHAAQGISHALSPIKALAQGGTAVGTGINADPRFARI
FADNLSQLSHVAFQPSDNFFYNLSSQDCVVGLSGQLKTAAVALMKIANDLRWMNSGPLAGLGEIELQGLQPGSSIMPGKV
NPVIPEAVTMVAAQVIGNDTTITVAGQSGNFQLNVMLPVIAHNLLESIELLTNACHALADKAIATFKVRHDNLEKALAKN
PILVTALNPVIGYLKAADIAKKAYQQGRAILDVAAEETDLDRATLEALLDPKKLTQGGIAH
>Mature_460_residues
TREYRIEKDSMGEVKVPADALYQAQTQRAVDNFTLSRYTMPSAFIAALAYIKQAAAMTNAQLGHLEGDIAQAIANASQAV
IDGQYAKQFPIDVFQTGSGTSSNMNANEVIATLASRELGYAVSPNDHVNMGQSSNDVIPTAIQVSAAINIEQQLLPALAH
LSQTLADKQSELADVVKTGRTHLMDAMPVTFAQELGGWKSQIDHAAQGISHALSPIKALAQGGTAVGTGINADPRFARIF
ADNLSQLSHVAFQPSDNFFYNLSSQDCVVGLSGQLKTAAVALMKIANDLRWMNSGPLAGLGEIELQGLQPGSSIMPGKVN
PVIPEAVTMVAAQVIGNDTTITVAGQSGNFQLNVMLPVIAHNLLESIELLTNACHALADKAIATFKVRHDNLEKALAKNP
ILVTALNPVIGYLKAADIAKKAYQQGRAILDVAAEETDLDRATLEALLDPKKLTQGGIAH

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=458, Percent_Identity=44.3231441048035, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI1787896, Length=455, Percent_Identity=45.9340659340659, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI87082375, Length=463, Percent_Identity=37.1490280777538, Blast_Score=289, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=42.9805615550756, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=42.3780487804878, Blast_Score=262, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=43.010752688172, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78710009, Length=460, Percent_Identity=42.8260869565217, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640177, Length=466, Percent_Identity=43.3476394849785, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640179, Length=466, Percent_Identity=43.3476394849785, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24662684, Length=461, Percent_Identity=42.7331887201735, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.9310344827586, Blast_Score=328, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 48910; Mature: 48779

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREYRIEKDSMGEVKVPADALYQAQTQRAVDNFTLSRYTMPSAFIAALAYIKQAAAMTN
CCCCEECCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHC
AQLGHLEGDIAQAIANASQAVIDGQYAKQFPIDVFQTGSGTSSNMNANEVIATLASRELG
CHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
YAVSPNDHVNMGQSSNDVIPTAIQVSAAINIEQQLLPALAHLSQTLADKQSELADVVKTG
CEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RTHLMDAMPVTFAQELGGWKSQIDHAAQGISHALSPIKALAQGGTAVGTGINADPRFARI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHH
FADNLSQLSHVAFQPSDNFFYNLSSQDCVVGLSGQLKTAAVALMKIANDLRWMNSGPLAG
HHHHHHHHHHHEECCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LGEIELQGLQPGSSIMPGKVNPVIPEAVTMVAAQVIGNDTTITVAGQSGNFQLNVMLPVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHH
AHNLLESIELLTNACHALADKAIATFKVRHDNLEKALAKNPILVTALNPVIGYLKAADIA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
KKAYQQGRAILDVAAEETDLDRATLEALLDPKKLTQGGIAH
HHHHHCCCEEEEECCCCCCCHHHHHHHHHCHHHHCCCCCCC
>Mature Secondary Structure 
TREYRIEKDSMGEVKVPADALYQAQTQRAVDNFTLSRYTMPSAFIAALAYIKQAAAMTN
CCCEECCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHC
AQLGHLEGDIAQAIANASQAVIDGQYAKQFPIDVFQTGSGTSSNMNANEVIATLASRELG
CHHCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
YAVSPNDHVNMGQSSNDVIPTAIQVSAAINIEQQLLPALAHLSQTLADKQSELADVVKTG
CEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RTHLMDAMPVTFAQELGGWKSQIDHAAQGISHALSPIKALAQGGTAVGTGINADPRFARI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHH
FADNLSQLSHVAFQPSDNFFYNLSSQDCVVGLSGQLKTAAVALMKIANDLRWMNSGPLAG
HHHHHHHHHHHEECCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LGEIELQGLQPGSSIMPGKVNPVIPEAVTMVAAQVIGNDTTITVAGQSGNFQLNVMLPVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEHHHH
AHNLLESIELLTNACHALADKAIATFKVRHDNLEKALAKNPILVTALNPVIGYLKAADIA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
KKAYQQGRAILDVAAEETDLDRATLEALLDPKKLTQGGIAH
HHHHHCCCEEEEECCCCCCCHHHHHHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8806672; 10984043 [H]