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The map label for this gene is nuoL [H]

Identifier: 227081734

GI number: 227081734

Start: 1639012

End: 1640562

Strand: Direct

Name: nuoL [H]

Synonym: VCM66_1521

Alternate gene names: 227081734

Gene position: 1639012-1640562 (Clockwise)

Preceding gene: 227081727

Following gene: 227081735

Centisome position: 56.66

GC content: 52.74

Gene sequence:

>1551_bases
ATGTCGGAACTGTCACCCCAGTGGCTCCTTGTTAGTTTGTATCTTGGTGGCCTTGTATTGAGCTTATGGTTACCTCAACA
GCAGTGGCGTATCGCCAAGTTCGCCACTCTCGCCGGCTTCATCGCCATGCTTGGAAATACCCTTTTTGCGATAGGGCAAG
CGAGCATCAACCTAGTCCCAGTCGATGGATTAGGCATCGTGATGGCGATACTCGTCTCGCTCCTAGGCTGGGTGATAACC
CGCTTCGCCCATCGTTATCTGCAAGGCGAAGCGCAGCAAGCGCGTTTTGTCACCGCCACGCTGTTTACCCTAAGCTGCGT
AGCCTTGTTGGTCACCAGTCAGCATCTGCTTGTGATCGTGCTGGCGTGGAGCGGAACCAGCGTTGGCCTACATCGTCTCC
TGACCTTTTACCCAGAGCGCAAAGCCGCACGCGTGGTGGCACACAAAAAATTCTTAGTCAGCCGTTTTGCCGAAATCTGT
TTGTTGTTAGCGCTCGGTTTGATCTATCTCGATGTCGGTAGCTTGTCGCTACAAGAGATTCACTTCGCGTTAAACGCGCA
GGATTCGCTGCCACTTAGCTTAACAGGGGCTGCGTTGTTACTCGCCTTCGCTGCTATCTTAAAAACCGCCCAGTTACCTC
TGCACGGCTGGCTGATTCAGGTGATGGAAGCGCCAACGCCAGTCTCGGCGTTATTGCATGCGGGTGTGGTTAACATGGGG
GGATTCGTTCTGCTGCGCGTCGCAGAACTGATTGGACTTGTTCCTTCGGCGCAATGGTTGCTGGTGATCGTCGGTAGCCT
CACTGCGGTGCTCGCTGGCATGGTCATGCTGACTCGGATCAGCATTAAAGTTCGTTTAGCTTGGTCAACGTGCGCGCAAA
TGGGCTTTATGCTGATGGAAATCGGCTTAGGCTTATATGAGCTCGCCCTGCTCCATTTGGTGGCGCATTCACTTTATAAA
GCTTATGCCTTCCTAAGTTCTGGCGAAGCGGTGGAGCGAGCACGTCTGCATGATTTTCAAGCCCCACAAAGTGTCAATGG
TAAAGCCGTGGGGGCTTTGCTCGCGGTAGGGCTCAGCGCAGCGCTTATCCTGATACTGCACCATTTATGGCAATCCTTAA
CACCCAATTTGCATCTACCCAATGCGGTGGTCGCGATTCTCGCCTTAGGTTTTGCTCCGCTACTTTGGCAAAGTGGACGT
TTTACACCAAGTGGCTTGAGCCTCGGTGTGCTGCGGGTTGCCCTACTCACCCAACTCTATTTCGTATGGCATCTGCTGTT
TGCGGGTTTAGCACCCGAGCTGCGCCCCGCTAGCCCACTCGCGCTGAGCTTTGTGATGTTCTGTTTTATCACCTTGTATC
TGATGCAAGTGCTGCTCTGCCTCTACCCCAAAAGCCGTTTATCACTGTGGCTCTATCCTTGGGTTTACCATGGTTTTTAT
CTTGATGAGACTTTTACGCGCCTGACCTTCAAATATTGGCCCGTGCAATTAACTCTGCCTACCGCTCTTTCATCAACTCA
AGCGCAACCGACGACAGGAGAACGCGTATGA

Upstream 100 bases:

>100_bases
AGAACGTTTTGTTAATAACAATATATTTTACTTACTCATTCCTTCGCAACCACAATAGCGCCACCGATAACATCTTATTT
TTCAATCAGGAGGCCGACCC

Downstream 100 bases:

>100_bases
ATGCGCCACGTTCGCTCTCAGCTTTAAGCACCACCGCTAACCCAGCATTATCGACACTTGTCGAACAAGTCTGCGCCTTG
ATTGCTCCTAACTGGCCGCT

Product: NADH dehydrogenase subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSELSPQWLLVSLYLGGLVLSLWLPQQQWRIAKFATLAGFIAMLGNTLFAIGQASINLVPVDGLGIVMAILVSLLGWVIT
RFAHRYLQGEAQQARFVTATLFTLSCVALLVTSQHLLVIVLAWSGTSVGLHRLLTFYPERKAARVVAHKKFLVSRFAEIC
LLLALGLIYLDVGSLSLQEIHFALNAQDSLPLSLTGAALLLAFAAILKTAQLPLHGWLIQVMEAPTPVSALLHAGVVNMG
GFVLLRVAELIGLVPSAQWLLVIVGSLTAVLAGMVMLTRISIKVRLAWSTCAQMGFMLMEIGLGLYELALLHLVAHSLYK
AYAFLSSGEAVERARLHDFQAPQSVNGKAVGALLAVGLSAALILILHHLWQSLTPNLHLPNAVVAILALGFAPLLWQSGR
FTPSGLSLGVLRVALLTQLYFVWHLLFAGLAPELRPASPLALSFVMFCFITLYLMQVLLCLYPKSRLSLWLYPWVYHGFY
LDETFTRLTFKYWPVQLTLPTALSSTQAQPTTGERV

Sequences:

>Translated_516_residues
MSELSPQWLLVSLYLGGLVLSLWLPQQQWRIAKFATLAGFIAMLGNTLFAIGQASINLVPVDGLGIVMAILVSLLGWVIT
RFAHRYLQGEAQQARFVTATLFTLSCVALLVTSQHLLVIVLAWSGTSVGLHRLLTFYPERKAARVVAHKKFLVSRFAEIC
LLLALGLIYLDVGSLSLQEIHFALNAQDSLPLSLTGAALLLAFAAILKTAQLPLHGWLIQVMEAPTPVSALLHAGVVNMG
GFVLLRVAELIGLVPSAQWLLVIVGSLTAVLAGMVMLTRISIKVRLAWSTCAQMGFMLMEIGLGLYELALLHLVAHSLYK
AYAFLSSGEAVERARLHDFQAPQSVNGKAVGALLAVGLSAALILILHHLWQSLTPNLHLPNAVVAILALGFAPLLWQSGR
FTPSGLSLGVLRVALLTQLYFVWHLLFAGLAPELRPASPLALSFVMFCFITLYLMQVLLCLYPKSRLSLWLYPWVYHGFY
LDETFTRLTFKYWPVQLTLPTALSSTQAQPTTGERV
>Mature_515_residues
SELSPQWLLVSLYLGGLVLSLWLPQQQWRIAKFATLAGFIAMLGNTLFAIGQASINLVPVDGLGIVMAILVSLLGWVITR
FAHRYLQGEAQQARFVTATLFTLSCVALLVTSQHLLVIVLAWSGTSVGLHRLLTFYPERKAARVVAHKKFLVSRFAEICL
LLALGLIYLDVGSLSLQEIHFALNAQDSLPLSLTGAALLLAFAAILKTAQLPLHGWLIQVMEAPTPVSALLHAGVVNMGG
FVLLRVAELIGLVPSAQWLLVIVGSLTAVLAGMVMLTRISIKVRLAWSTCAQMGFMLMEIGLGLYELALLHLVAHSLYKA
YAFLSSGEAVERARLHDFQAPQSVNGKAVGALLAVGLSAALILILHHLWQSLTPNLHLPNAVVAILALGFAPLLWQSGRF
TPSGLSLGVLRVALLTQLYFVWHLLFAGLAPELRPASPLALSFVMFCFITLYLMQVLLCLYPKSRLSLWLYPWVYHGFYL
DETFTRLTFKYWPVQLTLPTALSSTQAQPTTGERV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=281, Percent_Identity=32.7402135231317, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1788614, Length=294, Percent_Identity=34.6938775510204, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788829, Length=301, Percent_Identity=29.5681063122924, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788831, Length=303, Percent_Identity=28.7128712871287, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788827, Length=283, Percent_Identity=28.2685512367491, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56462; Mature: 56331

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELSPQWLLVSLYLGGLVLSLWLPQQQWRIAKFATLAGFIAMLGNTLFAIGQASINLVP
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VDGLGIVMAILVSLLGWVITRFAHRYLQGEAQQARFVTATLFTLSCVALLVTSQHLLVIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LAWSGTSVGLHRLLTFYPERKAARVVAHKKFLVSRFAEICLLLALGLIYLDVGSLSLQEI
EECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
HFALNAQDSLPLSLTGAALLLAFAAILKTAQLPLHGWLIQVMEAPTPVSALLHAGVVNMG
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC
GFVLLRVAELIGLVPSAQWLLVIVGSLTAVLAGMVMLTRISIKVRLAWSTCAQMGFMLME
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHH
IGLGLYELALLHLVAHSLYKAYAFLSSGEAVERARLHDFQAPQSVNGKAVGALLAVGLSA
HCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ALILILHHLWQSLTPNLHLPNAVVAILALGFAPLLWQSGRFTPSGLSLGVLRVALLTQLY
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
FVWHLLFAGLAPELRPASPLALSFVMFCFITLYLMQVLLCLYPKSRLSLWLYPWVYHGFY
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEHHHHHCHH
LDETFTRLTFKYWPVQLTLPTALSSTQAQPTTGERV
HHHHHHHHEEEEEEEEEEECCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SELSPQWLLVSLYLGGLVLSLWLPQQQWRIAKFATLAGFIAMLGNTLFAIGQASINLVP
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VDGLGIVMAILVSLLGWVITRFAHRYLQGEAQQARFVTATLFTLSCVALLVTSQHLLVIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LAWSGTSVGLHRLLTFYPERKAARVVAHKKFLVSRFAEICLLLALGLIYLDVGSLSLQEI
EECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
HFALNAQDSLPLSLTGAALLLAFAAILKTAQLPLHGWLIQVMEAPTPVSALLHAGVVNMG
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCC
GFVLLRVAELIGLVPSAQWLLVIVGSLTAVLAGMVMLTRISIKVRLAWSTCAQMGFMLME
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHH
IGLGLYELALLHLVAHSLYKAYAFLSSGEAVERARLHDFQAPQSVNGKAVGALLAVGLSA
HCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ALILILHHLWQSLTPNLHLPNAVVAILALGFAPLLWQSGRFTPSGLSLGVLRVALLTQLY
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
FVWHLLFAGLAPELRPASPLALSFVMFCFITLYLMQVLLCLYPKSRLSLWLYPWVYHGFY
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEHHHHHCHH
LDETFTRLTFKYWPVQLTLPTALSSTQAQPTTGERV
HHHHHHHHEEEEEEEEEEECCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]