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The map label for this gene is tolC [H]

Identifier: 227081719

GI number: 227081719

Start: 1624551

End: 1625810

Strand: Direct

Name: tolC [H]

Synonym: VCM66_1505

Alternate gene names: 227081719

Gene position: 1624551-1625810 (Clockwise)

Preceding gene: 227081718

Following gene: 227081720

Centisome position: 56.16

GC content: 49.05

Gene sequence:

>1260_bases
ATGAATAAGGGCTTTCTCACCGCATGTGTACTGACTAGCCTGATGGCATCTTCTTCTGCTTTTGCGCTCTCCATCGAGCA
AGCGTGGCAGCAAGCCAAGCAACAATCACCCGATGCACACATTGCCGAACTCTCGGTGCAGCTCAGCGAGCAAGATAAGT
ATCTCGCCAAAAGCGATCTCCTTCCTTCACTCTCAGGCAGTGCCGGAATGAACTGGTCAGAAAGCCAAAATGGCTCGAGC
AGTTATGGCGCCACCATTGAGCAAACCTTGTGGGATAGCCGAAAATGGAGCGCGTTGGATAAAGCCGATGCGGACTGGGT
GTTGGCCCAACTTGAACGTAACCAAGCTTATAACCAACTCGCTCATCAGTTACTCAGTGCTTATTTTGCCTTAGCGGAAG
CGCAAAGTAATTTGACTCTTGCCCAGCAAAAACAAGCGGATGGCGCACAATTATTAGCCATCGCTGAACAGCGTTATCTG
GCGGGTAAAATAAAATCTGTGGAGTTGGAAGAGCTACGCGTCAACCAACTTGATGAAGAGTCAACCATTCTCAATGCGCA
GGCGGAACTCGAAACCAAACGCAGCCAACTCTCAATACTGATCAATGCCTCAGCCCCGCAAGTGGATGAAGTGGATACCC
AGACGCCAACACCGAGTTGGCCATTCAGTGCTGAACTGGATGACTGGCTTACCGCCGCCAAAGATCATAGCCCTGAGCTC
TTGATTGCTATCCAAAAAGTGGCGATCAGCCAAATTGCTAAGCAGCAGAGTCAAAGTGGGTATTATCCGAGTTTGCGCGC
CTCTGCAGGCTATCAAGACGGCGATCAACGAAACGGTGAATTCAATGCCGGTCTCACGCTCTCCATCCCGATTGATTTAA
ATGGAGCAACCCGCAGTGATAACGAAAAAGCTCATCTCAACTGGTTGATGGCTCAGCAACAGCAACGCAAAGTCGAGATC
GGATTACATCAAAATTTGCGTCAACAAATTCAGCAGGTTGAGATTGAATGGCAGCAAATTACCATGGCGGATCAGCAAGT
GACCCATCGCGATAAAGTGCTGCGCAGTAAACAGCAACTGTTTGATGCTGGCTTAGCCGAAGCCTCCGATTTAATCGATG
TGCACAACAGCCTATTTCGAGCCAAACATAATCTGGCCAGTCGTTGGTATAGCTATTGGCGTCAGCGCATTGAGCTACTC
AAATTAACAGGTCAGTTGAACGATAATGCCATCGCTCAGCTTTCAGGAGCCTTTCGTTAA

Upstream 100 bases:

>100_bases
CCGGTCAAGCTTGGGCTTTCTGACGGCGTACAGGTTGAGATCTTAGAAGGCGTAGCCGAAAAAGACAGCATAGTGGATAA
CAGCATGCTGGGAGGCCAGC

Downstream 100 bases:

>100_bases
TGCGCCTCTTGTTACAGCAATCTTGGCAAACTTTGCTCGCTCATCGCCTGAAAAGCGTATTGGCGATCACCGCGATTAGC
TGGGGGGTGATTTCTGTGGT

Product: putative outer membrane protein TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNKGFLTACVLTSLMASSSAFALSIEQAWQQAKQQSPDAHIAELSVQLSEQDKYLAKSDLLPSLSGSAGMNWSESQNGSS
SYGATIEQTLWDSRKWSALDKADADWVLAQLERNQAYNQLAHQLLSAYFALAEAQSNLTLAQQKQADGAQLLAIAEQRYL
AGKIKSVELEELRVNQLDEESTILNAQAELETKRSQLSILINASAPQVDEVDTQTPTPSWPFSAELDDWLTAAKDHSPEL
LIAIQKVAISQIAKQQSQSGYYPSLRASAGYQDGDQRNGEFNAGLTLSIPIDLNGATRSDNEKAHLNWLMAQQQQRKVEI
GLHQNLRQQIQQVEIEWQQITMADQQVTHRDKVLRSKQQLFDAGLAEASDLIDVHNSLFRAKHNLASRWYSYWRQRIELL
KLTGQLNDNAIAQLSGAFR

Sequences:

>Translated_419_residues
MNKGFLTACVLTSLMASSSAFALSIEQAWQQAKQQSPDAHIAELSVQLSEQDKYLAKSDLLPSLSGSAGMNWSESQNGSS
SYGATIEQTLWDSRKWSALDKADADWVLAQLERNQAYNQLAHQLLSAYFALAEAQSNLTLAQQKQADGAQLLAIAEQRYL
AGKIKSVELEELRVNQLDEESTILNAQAELETKRSQLSILINASAPQVDEVDTQTPTPSWPFSAELDDWLTAAKDHSPEL
LIAIQKVAISQIAKQQSQSGYYPSLRASAGYQDGDQRNGEFNAGLTLSIPIDLNGATRSDNEKAHLNWLMAQQQQRKVEI
GLHQNLRQQIQQVEIEWQQITMADQQVTHRDKVLRSKQQLFDAGLAEASDLIDVHNSLFRAKHNLASRWYSYWRQRIELL
KLTGQLNDNAIAQLSGAFR
>Mature_419_residues
MNKGFLTACVLTSLMASSSAFALSIEQAWQQAKQQSPDAHIAELSVQLSEQDKYLAKSDLLPSLSGSAGMNWSESQNGSS
SYGATIEQTLWDSRKWSALDKADADWVLAQLERNQAYNQLAHQLLSAYFALAEAQSNLTLAQQKQADGAQLLAIAEQRYL
AGKIKSVELEELRVNQLDEESTILNAQAELETKRSQLSILINASAPQVDEVDTQTPTPSWPFSAELDDWLTAAKDHSPEL
LIAIQKVAISQIAKQQSQSGYYPSLRASAGYQDGDQRNGEFNAGLTLSIPIDLNGATRSDNEKAHLNWLMAQQQQRKVEI
GLHQNLRQQIQQVEIEWQQITMADQQVTHRDKVLRSKQQLFDAGLAEASDLIDVHNSLFRAKHNLASRWYSYWRQRIELL
KLTGQLNDNAIAQLSGAFR

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=435, Percent_Identity=23.6781609195402, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 46737; Mature: 46737

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGFLTACVLTSLMASSSAFALSIEQAWQQAKQQSPDAHIAELSVQLSEQDKYLAKSDL
CCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCEEHHHEEEECHHHHHHHHHHC
LPSLSGSAGMNWSESQNGSSSYGATIEQTLWDSRKWSALDKADADWVLAQLERNQAYNQL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AHQLLSAYFALAEAQSNLTLAQQKQADGAQLLAIAEQRYLAGKIKSVELEELRVNQLDEE
HHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCH
STILNAQAELETKRSQLSILINASAPQVDEVDTQTPTPSWPFSAELDDWLTAAKDHSPEL
HHHCCCHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHH
LIAIQKVAISQIAKQQSQSGYYPSLRASAGYQDGDQRNGEFNAGLTLSIPIDLNGATRSD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
NEKAHLNWLMAQQQQRKVEIGLHQNLRQQIQQVEIEWQQITMADQQVTHRDKVLRSKQQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDAGLAEASDLIDVHNSLFRAKHNLASRWYSYWRQRIELLKLTGQLNDNAIAQLSGAFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MNKGFLTACVLTSLMASSSAFALSIEQAWQQAKQQSPDAHIAELSVQLSEQDKYLAKSDL
CCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCEEHHHEEEECHHHHHHHHHHC
LPSLSGSAGMNWSESQNGSSSYGATIEQTLWDSRKWSALDKADADWVLAQLERNQAYNQL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AHQLLSAYFALAEAQSNLTLAQQKQADGAQLLAIAEQRYLAGKIKSVELEELRVNQLDEE
HHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCH
STILNAQAELETKRSQLSILINASAPQVDEVDTQTPTPSWPFSAELDDWLTAAKDHSPEL
HHHCCCHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHH
LIAIQKVAISQIAKQQSQSGYYPSLRASAGYQDGDQRNGEFNAGLTLSIPIDLNGATRSD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
NEKAHLNWLMAQQQQRKVEIGLHQNLRQQIQQVEIEWQQITMADQQVTHRDKVLRSKQQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDAGLAEASDLIDVHNSLFRAKHNLASRWYSYWRQRIELLKLTGQLNDNAIAQLSGAFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]