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The map label for this gene is macA [H]

Identifier: 227081718

GI number: 227081718

Start: 1623398

End: 1624558

Strand: Direct

Name: macA [H]

Synonym: VCM66_1504

Alternate gene names: 227081718

Gene position: 1623398-1624558 (Clockwise)

Preceding gene: 227081717

Following gene: 227081719

Centisome position: 56.12

GC content: 47.98

Gene sequence:

>1161_bases
TTGATTGATGAAATAAATGAGTTTTTTATGGCAAAACGTTGGACACTTCTTGCAACCTTAGTAGGTGTTGGCTTGGCAGG
TGGTTATTACACTCTCACTTATACCCCTGAGTCTTCATCCCCATTACCGACCCTCAACGTCAGCCGGGGAACAATAGAGA
AACAGGCGGTCGCGGTTGGTCAAATCATGCCTTCACATTCGGTGGCAATTAAATCGCAAATCAACGGTATTGTCGGTGAA
ATTTATGTGCGTGAAGGGCAACATGTTGAGCAAGGACAACCCTTGATTAAGGTTCGTCCGAATCCAACACCACAGGCGTT
GACCGATGCCTCCACAGAACTGATGCAAAGTGAAGCGAATCTTGAATCAGACTTACAACGCTTAGCCAACCTTGAGCGCT
TAGTGAAACAGCAAATCATTCCTGCCAATTATGATGATTACGTCCGCGCGAAAGCCGACGTCAAAGCTAAGCAAGCCGAA
GTGCAGCAAAAACGCCAAAACCTAGAGTTGATCCGCAGCGGTGAAGCTTCGATTGGGCAAGCCAAGCTCTCCTCTACTCT
ATATGCCCCGATCAGTGGCACGATTTTAAATATCAAAGTCGAGGTCGGAGAGCCGATCATCTCTACCGAGTCGAGCCAAG
CGGCAACAGAAATGATCCAGTTAGCGGATATGAAAAATATCGTGTTTAAAGGCAGTGTCAGTGAACATGATGCTGCGCAG
CTGAATGCCGGCATGCCCGCCAGTATTACCGTTGCCCCCTACCCTGAGCAGGTGATCAATGGTCAGTTGACCAAAGTCGC
GATTCAATCGGAGCGCTTAAATAATCCGGAAAACAAAGATGGAAAAAGTTTTGATAACGGATTTCAAGTCGAAATCGGAC
AATTAGACATCCCTGAAAACATGACATTACGTTCTGGATTCTCTGCCAGCGCAAAAATCACCTTACAACGTGCCGCGGAT
GTTATTACCCTTCCGGAGCGCGCATTACAGTTTGAGGGCGAAACGCCCATGGTGCTGATTGCGGATGGCTCTGCACAAGG
CTATACCGCTCATCCGGTCAAGCTTGGGCTTTCTGACGGCGTACAGGTTGAGATCTTAGAAGGCGTAGCCGAAAAAGACA
GCATAGTGGATAACAGCATGCTGGGAGGCCAGCATGAATAA

Upstream 100 bases:

>100_bases
ATACAGCATGGACAGGGCTTTTTATTTTTCCCTTCGCATGTTAAAAAACAACGTTTTTTTGAACTTTCCTTATCATCCTT
AGTCTGAATCCCGTCTCTAA

Downstream 100 bases:

>100_bases
GGGCTTTCTCACCGCATGTGTACTGACTAGCCTGATGGCATCTTCTTCTGCTTTTGCGCTCTCCATCGAGCAAGCGTGGC
AGCAAGCCAAGCAACAATCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MIDEINEFFMAKRWTLLATLVGVGLAGGYYTLTYTPESSSPLPTLNVSRGTIEKQAVAVGQIMPSHSVAIKSQINGIVGE
IYVREGQHVEQGQPLIKVRPNPTPQALTDASTELMQSEANLESDLQRLANLERLVKQQIIPANYDDYVRAKADVKAKQAE
VQQKRQNLELIRSGEASIGQAKLSSTLYAPISGTILNIKVEVGEPIISTESSQAATEMIQLADMKNIVFKGSVSEHDAAQ
LNAGMPASITVAPYPEQVINGQLTKVAIQSERLNNPENKDGKSFDNGFQVEIGQLDIPENMTLRSGFSASAKITLQRAAD
VITLPERALQFEGETPMVLIADGSAQGYTAHPVKLGLSDGVQVEILEGVAEKDSIVDNSMLGGQHE

Sequences:

>Translated_386_residues
MIDEINEFFMAKRWTLLATLVGVGLAGGYYTLTYTPESSSPLPTLNVSRGTIEKQAVAVGQIMPSHSVAIKSQINGIVGE
IYVREGQHVEQGQPLIKVRPNPTPQALTDASTELMQSEANLESDLQRLANLERLVKQQIIPANYDDYVRAKADVKAKQAE
VQQKRQNLELIRSGEASIGQAKLSSTLYAPISGTILNIKVEVGEPIISTESSQAATEMIQLADMKNIVFKGSVSEHDAAQ
LNAGMPASITVAPYPEQVINGQLTKVAIQSERLNNPENKDGKSFDNGFQVEIGQLDIPENMTLRSGFSASAKITLQRAAD
VITLPERALQFEGETPMVLIADGSAQGYTAHPVKLGLSDGVQVEILEGVAEKDSIVDNSMLGGQHE
>Mature_386_residues
MIDEINEFFMAKRWTLLATLVGVGLAGGYYTLTYTPESSSPLPTLNVSRGTIEKQAVAVGQIMPSHSVAIKSQINGIVGE
IYVREGQHVEQGQPLIKVRPNPTPQALTDASTELMQSEANLESDLQRLANLERLVKQQIIPANYDDYVRAKADVKAKQAE
VQQKRQNLELIRSGEASIGQAKLSSTLYAPISGTILNIKVEVGEPIISTESSQAATEMIQLADMKNIVFKGSVSEHDAAQ
LNAGMPASITVAPYPEQVINGQLTKVAIQSERLNNPENKDGKSFDNGFQVEIGQLDIPENMTLRSGFSASAKITLQRAAD
VITLPERALQFEGETPMVLIADGSAQGYTAHPVKLGLSDGVQVEILEGVAEKDSIVDNSMLGGQHE

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=391, Percent_Identity=24.5524296675192, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082036, Length=369, Percent_Identity=21.680216802168, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41692; Mature: 41692

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDEINEFFMAKRWTLLATLVGVGLAGGYYTLTYTPESSSPLPTLNVSRGTIEKQAVAVG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHH
QIMPSHSVAIKSQINGIVGEIYVREGQHVEQGQPLIKVRPNPTPQALTDASTELMQSEAN
HCCCCCCEEHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
LESDLQRLANLERLVKQQIIPANYDDYVRAKADVKAKQAEVQQKRQNLELIRSGEASIGQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AKLSSTLYAPISGTILNIKVEVGEPIISTESSQAATEMIQLADMKNIVFKGSVSEHDAAQ
HHHCCEEECCCCCEEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHH
LNAGMPASITVAPYPEQVINGQLTKVAIQSERLNNPENKDGKSFDNGFQVEIGQLDIPEN
HCCCCCCEEEECCCCHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCCCCEEEECCEECCCC
MTLRSGFSASAKITLQRAADVITLPERALQFEGETPMVLIADGSAQGYTAHPVKLGLSDG
CEECCCCCCCEEEEEECCCCEEECCHHHHCCCCCCCEEEEECCCCCCEEECCEEECCCCC
VQVEILEGVAEKDSIVDNSMLGGQHE
CEEEEECCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIDEINEFFMAKRWTLLATLVGVGLAGGYYTLTYTPESSSPLPTLNVSRGTIEKQAVAVG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHH
QIMPSHSVAIKSQINGIVGEIYVREGQHVEQGQPLIKVRPNPTPQALTDASTELMQSEAN
HCCCCCCEEHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
LESDLQRLANLERLVKQQIIPANYDDYVRAKADVKAKQAEVQQKRQNLELIRSGEASIGQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
AKLSSTLYAPISGTILNIKVEVGEPIISTESSQAATEMIQLADMKNIVFKGSVSEHDAAQ
HHHCCEEECCCCCEEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHH
LNAGMPASITVAPYPEQVINGQLTKVAIQSERLNNPENKDGKSFDNGFQVEIGQLDIPEN
HCCCCCCEEEECCCCHHHHCCCEEEEEEEHHHCCCCCCCCCCCCCCCCEEEECCEECCCC
MTLRSGFSASAKITLQRAADVITLPERALQFEGETPMVLIADGSAQGYTAHPVKLGLSDG
CEECCCCCCCEEEEEECCCCEEECCHHHHCCCCCCCEEEEECCCCCCEEECCEEECCCCC
VQVEILEGVAEKDSIVDNSMLGGQHE
CEEEEECCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]