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The map label for this gene is norM [H]

Identifier: 227081695

GI number: 227081695

Start: 1600131

End: 1601516

Strand: Direct

Name: norM [H]

Synonym: VCM66_1481

Alternate gene names: 227081695

Gene position: 1600131-1601516 (Clockwise)

Preceding gene: 227081694

Following gene: 227081696

Centisome position: 55.32

GC content: 49.06

Gene sequence:

>1386_bases
TTGGAGAACTCTGTGCATCGTTACAAAAAAGAAGCATCAAATTTAATTAAACTCGCGACACCCGTACTGATTGCCTCAGT
CGCGCAAACGGGTATGGGCTTTGTCGATACCATCATGGCGGGTGGTGTCAGTGCGATAGACATGGCCGCGGTATCGATCG
CAGCCAGTATTTGGCTACCTTCCATTTTGTTTGGCGTGGGTTTGCTGATGGCTTTAGTGCCGGTCGTTGCCCAGCTCAAT
GGCGCAGGTCGCCAACACAAGATTCCGTTTGAAGTTCATCAGGGGTTGATCCTCGCGTTATTGGTTTCGGTCCCGATCAT
TGCGGTACTCTTTCAAACTCAATTCATCATTCGGTTTATGGATGTAGAAGAGGCAATGGCGACCAAAACCGTCGGTTATA
TGCACGCCGTCATTTTTGCGGTCCCCGCTTACTTACTGTTTCAGGCACTGCGCAGTTTTACCGATGGTATGTCACTGACC
AAACCTGCGATGGTGATTGGTTTTATTGGGTTATTGCTCAATATCCCTCTCAACTGGATTTTCGTTTACGGGAAATTTGG
CGCACCAGAGCTAGGCGGTGTGGGTTGTGGCGTTGCAACCGCGATTGTGTATTGGATCATGTTGCTGCTGCTACTGTTTT
ACATCGTGACCTCTAAGCGTCTGGCACATGTAAAAGTGTTTGAGACTTTCCATAAACCACAGCCAAAAGAGTTGATCCGT
CTATTTCGCTTAGGTTTTCCTGTTGCAGCCGCCCTCTTTTTTGAAGTGACTCTTTTTGCTGTGGTCGCACTTTTAGTGGC
ACCGTTAGGGTCAACTGTCGTGGCGGCACACCAAGTGGCGCTCAATTTCTCTTCTTTGGTGTTTATGTTCCCAATGAGTA
TTGGTGCCGCGGTCTCCATTCGTGTTGGCCATAAGTTAGGTGAGCAAGACACCAAAGGCGCTGCAATTGCAGCCAATGTG
GGTTTAATGACTGGCCTTGCCACTGCGTGTATTACCGCGTTGCTCACCGTGTTGTTTCGTGAACAGATCGCGCTGCTGTA
TACCGAAAACCAAGTGGTGGTAGCGTTAGCGATGCAGCTCTTACTGTTTGCGGCAATCTATCAATGTATGGATGCGGTAC
AAGTTGTCGCCGCAGGATCACTGCGTGGCTATAAAGATATGACAGCGATTTTCCACCGTACATTTATCTCCTACTGGGTA
CTTGGCTTGCCCACCGGTTATATCCTAGGGATGACCAACTGGCTGACGGAGCAACCCTTAGGTGCCAAAGGTTTCTGGCT
CGGATTTATCATCGGACTCTCGGCGGCCGCACTGATGCTAGGACAGCGTTTGTACTGGCTGCAAAAACAGAGTGATGACG
TACAGTTACACCTTGCAGCAAAATAA

Upstream 100 bases:

>100_bases
TTCGTGGAGCTTAATGCGACATTTCATTATCATGCCATCCATTGCCATAAGTTGAGTATCCCGCGACTCACTGTTTAGGC
TCCTCTTTGCTTGTCCCTCT

Downstream 100 bases:

>100_bases
TCGATAGAAAATCCCCTCAAGGCCAGCCAATCTATGCTGGCCTTTTTATTGATTGAGAAGAACCATAAACGCAGTATTAC
GTATATTTAAAGAACTTGGC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MENSVHRYKKEASNLIKLATPVLIASVAQTGMGFVDTIMAGGVSAIDMAAVSIAASIWLPSILFGVGLLMALVPVVAQLN
GAGRQHKIPFEVHQGLILALLVSVPIIAVLFQTQFIIRFMDVEEAMATKTVGYMHAVIFAVPAYLLFQALRSFTDGMSLT
KPAMVIGFIGLLLNIPLNWIFVYGKFGAPELGGVGCGVATAIVYWIMLLLLLFYIVTSKRLAHVKVFETFHKPQPKELIR
LFRLGFPVAAALFFEVTLFAVVALLVAPLGSTVVAAHQVALNFSSLVFMFPMSIGAAVSIRVGHKLGEQDTKGAAIAANV
GLMTGLATACITALLTVLFREQIALLYTENQVVVALAMQLLLFAAIYQCMDAVQVVAAGSLRGYKDMTAIFHRTFISYWV
LGLPTGYILGMTNWLTEQPLGAKGFWLGFIIGLSAAALMLGQRLYWLQKQSDDVQLHLAAK

Sequences:

>Translated_461_residues
MENSVHRYKKEASNLIKLATPVLIASVAQTGMGFVDTIMAGGVSAIDMAAVSIAASIWLPSILFGVGLLMALVPVVAQLN
GAGRQHKIPFEVHQGLILALLVSVPIIAVLFQTQFIIRFMDVEEAMATKTVGYMHAVIFAVPAYLLFQALRSFTDGMSLT
KPAMVIGFIGLLLNIPLNWIFVYGKFGAPELGGVGCGVATAIVYWIMLLLLLFYIVTSKRLAHVKVFETFHKPQPKELIR
LFRLGFPVAAALFFEVTLFAVVALLVAPLGSTVVAAHQVALNFSSLVFMFPMSIGAAVSIRVGHKLGEQDTKGAAIAANV
GLMTGLATACITALLTVLFREQIALLYTENQVVVALAMQLLLFAAIYQCMDAVQVVAAGSLRGYKDMTAIFHRTFISYWV
LGLPTGYILGMTNWLTEQPLGAKGFWLGFIIGLSAAALMLGQRLYWLQKQSDDVQLHLAAK
>Mature_461_residues
MENSVHRYKKEASNLIKLATPVLIASVAQTGMGFVDTIMAGGVSAIDMAAVSIAASIWLPSILFGVGLLMALVPVVAQLN
GAGRQHKIPFEVHQGLILALLVSVPIIAVLFQTQFIIRFMDVEEAMATKTVGYMHAVIFAVPAYLLFQALRSFTDGMSLT
KPAMVIGFIGLLLNIPLNWIFVYGKFGAPELGGVGCGVATAIVYWIMLLLLLFYIVTSKRLAHVKVFETFHKPQPKELIR
LFRLGFPVAAALFFEVTLFAVVALLVAPLGSTVVAAHQVALNFSSLVFMFPMSIGAAVSIRVGHKLGEQDTKGAAIAANV
GLMTGLATACITALLTVLFREQIALLYTENQVVVALAMQLLLFAAIYQCMDAVQVVAAGSLRGYKDMTAIFHRTFISYWV
LGLPTGYILGMTNWLTEQPLGAKGFWLGFIIGLSAAALMLGQRLYWLQKQSDDVQLHLAAK

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide and acriflavine [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=458, Percent_Identity=24.0174672489083, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI153792564, Length=443, Percent_Identity=23.2505643340858, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI38261960, Length=479, Percent_Identity=22.1294363256785, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI48994890, Length=458, Percent_Identity=55.4585152838428, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI226510954, Length=448, Percent_Identity=23.8839285714286, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321821, Length=384, Percent_Identity=23.1770833333333, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320545, Length=437, Percent_Identity=23.1121281464531, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49854; Mature: 49854

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSVHRYKKEASNLIKLATPVLIASVAQTGMGFVDTIMAGGVSAIDMAAVSIAASIWLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SILFGVGLLMALVPVVAQLNGAGRQHKIPFEVHQGLILALLVSVPIIAVLFQTQFIIRFM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVEEAMATKTVGYMHAVIFAVPAYLLFQALRSFTDGMSLTKPAMVIGFIGLLLNIPLNWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEE
FVYGKFGAPELGGVGCGVATAIVYWIMLLLLLFYIVTSKRLAHVKVFETFHKPQPKELIR
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
LFRLGFPVAAALFFEVTLFAVVALLVAPLGSTVVAAHQVALNFSSLVFMFPMSIGAAVSI
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RVGHKLGEQDTKGAAIAANVGLMTGLATACITALLTVLFREQIALLYTENQVVVALAMQL
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHH
LLFAAIYQCMDAVQVVAAGSLRGYKDMTAIFHRTFISYWVLGLPTGYILGMTNWLTEQPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GAKGFWLGFIIGLSAAALMLGQRLYWLQKQSDDVQLHLAAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MENSVHRYKKEASNLIKLATPVLIASVAQTGMGFVDTIMAGGVSAIDMAAVSIAASIWLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SILFGVGLLMALVPVVAQLNGAGRQHKIPFEVHQGLILALLVSVPIIAVLFQTQFIIRFM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVEEAMATKTVGYMHAVIFAVPAYLLFQALRSFTDGMSLTKPAMVIGFIGLLLNIPLNWI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEE
FVYGKFGAPELGGVGCGVATAIVYWIMLLLLLFYIVTSKRLAHVKVFETFHKPQPKELIR
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHH
LFRLGFPVAAALFFEVTLFAVVALLVAPLGSTVVAAHQVALNFSSLVFMFPMSIGAAVSI
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RVGHKLGEQDTKGAAIAANVGLMTGLATACITALLTVLFREQIALLYTENQVVVALAMQL
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHH
LLFAAIYQCMDAVQVVAAGSLRGYKDMTAIFHRTFISYWVLGLPTGYILGMTNWLTEQPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GAKGFWLGFIIGLSAAALMLGQRLYWLQKQSDDVQLHLAAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11583854; 10952301 [H]