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The map label for this gene is yrhP [H]

Identifier: 227081592

GI number: 227081592

Start: 1475688

End: 1476326

Strand: Direct

Name: yrhP [H]

Synonym: VCM66_1376

Alternate gene names: 227081592

Gene position: 1475688-1476326 (Clockwise)

Preceding gene: 227081591

Following gene: 227081594

Centisome position: 51.02

GC content: 44.6

Gene sequence:

>639_bases
ATGGTTATCCAGAATTTCGAAGCTTTTCTTATCGCTATTACTATTTTGACTTTAACACCGGGGCTAGATACTGCTTTGGT
GATCCGTAACACTTCGCGGGCTGGGTTTGCCGATGGATGCACGACCAGCTTGGGTATCTGTTTTGGCTTATTCGTTCACG
CCACTTTTTCTGCTATTGGTATCTCTGCGATTCTTGCTCAATCGGCTGAGCTATTTCAAATCGTTAAAATGGTAGGAGCG
GCTTATCTGATTTGGTTGGGTATTTCGAGCTTACGATCATTAATGAAAACAGGGCAGGGCATTGAGGTTGCGAGCCTAGC
TCATGCACAATTTCGCTTAACCCGATCGCTGCGTGAAGGTTTCCTCTCGAATGTGCTGAACCCGAAAACTGCCGTCTTTT
ACTTAGCTTTTCTGCCTCAATTTATTAATCCTGACTATTCACCGTTAGCGCAATCTTTGCTGATGGCGTTGATTCATTTT
GCGATTGCGATGGTATGGCAATGTGGTTTAGCCGGTGCTTTGAGTAGCGCTAAAAACCTTCTGAAAAACGCTTCTTTTAT
GCGTTGGATGGAAGGTACAACGGGAGTTGTTTTGGTCGCATTAGGTATCAAACTGCTCTTAGAGAAACCTCAAGCCTAG

Upstream 100 bases:

>100_bases
GCTTGGCGAGTAACTTAGCGTGAATTGAAAAATCTTCATAAGACACATGAGAGGCTTTGCGGTATAAGAAAGGCTTGCTG
ATTTTCCTTCAAGGATTGAT

Downstream 100 bases:

>100_bases
CGTGTAATAAAGGTTTAAAAGTAGAAAAAGCGCCGTTGGGCGCTTTTTCTTTGTGCTTAGACCGACTAGGTGTTATTGAA
CTTGGGCTTCGGCTTCGGAG

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MVIQNFEAFLIAITILTLTPGLDTALVIRNTSRAGFADGCTTSLGICFGLFVHATFSAIGISAILAQSAELFQIVKMVGA
AYLIWLGISSLRSLMKTGQGIEVASLAHAQFRLTRSLREGFLSNVLNPKTAVFYLAFLPQFINPDYSPLAQSLLMALIHF
AIAMVWQCGLAGALSSAKNLLKNASFMRWMEGTTGVVLVALGIKLLLEKPQA

Sequences:

>Translated_212_residues
MVIQNFEAFLIAITILTLTPGLDTALVIRNTSRAGFADGCTTSLGICFGLFVHATFSAIGISAILAQSAELFQIVKMVGA
AYLIWLGISSLRSLMKTGQGIEVASLAHAQFRLTRSLREGFLSNVLNPKTAVFYLAFLPQFINPDYSPLAQSLLMALIHF
AIAMVWQCGLAGALSSAKNLLKNASFMRWMEGTTGVVLVALGIKLLLEKPQA
>Mature_212_residues
MVIQNFEAFLIAITILTLTPGLDTALVIRNTSRAGFADGCTTSLGICFGLFVHATFSAIGISAILAQSAELFQIVKMVGA
AYLIWLGISSLRSLMKTGQGIEVASLAHAQFRLTRSLREGFLSNVLNPKTAVFYLAFLPQFINPDYSPLAQSLLMALIHF
AIAMVWQCGLAGALSSAKNLLKNASFMRWMEGTTGVVLVALGIKLLLEKPQA

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=171, Percent_Identity=30.4093567251462, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788099, Length=143, Percent_Identity=27.2727272727273, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI48994978, Length=195, Percent_Identity=24.1025641025641, Blast_Score=60, Evalue=8e-11,
Organism=Escherichia coli, GI1786522, Length=191, Percent_Identity=22.5130890052356, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22798; Mature: 22798

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIQNFEAFLIAITILTLTPGLDTALVIRNTSRAGFADGCTTSLGICFGLFVHATFSAIG
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ISAILAQSAELFQIVKMVGAAYLIWLGISSLRSLMKTGQGIEVASLAHAQFRLTRSLREG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FLSNVLNPKTAVFYLAFLPQFINPDYSPLAQSLLMALIHFAIAMVWQCGLAGALSSAKNL
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKNASFMRWMEGTTGVVLVALGIKLLLEKPQA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVIQNFEAFLIAITILTLTPGLDTALVIRNTSRAGFADGCTTSLGICFGLFVHATFSAIG
CCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ISAILAQSAELFQIVKMVGAAYLIWLGISSLRSLMKTGQGIEVASLAHAQFRLTRSLREG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FLSNVLNPKTAVFYLAFLPQFINPDYSPLAQSLLMALIHFAIAMVWQCGLAGALSSAKNL
HHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKNASFMRWMEGTTGVVLVALGIKLLLEKPQA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9308178 [H]