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The map label for this gene is 227081585

Identifier: 227081585

GI number: 227081585

Start: 1465302

End: 1466876

Strand: Reverse

Name: 227081585

Synonym: VCM66_1369

Alternate gene names: NA

Gene position: 1466876-1465302 (Counterclockwise)

Preceding gene: 227081593

Following gene: 227081584

Centisome position: 50.71

GC content: 48.13

Gene sequence:

>1575_bases
TTGATGAAAAAACGCGCTTCTCATCACTTGGTTATCTGCACCTTGATTGATACACTTCGCTTTCGTTTATCGTCACGGAA
GGCTATTTCCATGAATGCGTTCAATAAATTAGTAAAACATTCTAAAAAAATTTCTAACTTTAATCATTTGTCGTCCATCG
TTGGTTGGGACCAAGCCGCCGTCATGCCCAGTGGCGGCGCTGAAGCGCGCTCGCAAGCCATGGCAGAGCTTTCTGTGCAT
ATCCATGGCCTGATGACCCAGCCACAATTGGCTGATTGGTTTGCCGAGGCAGAAAGTGAAACGCTCAATATCGAGCAGCA
AGCCACCCTGCGCGAAATGAAACGCCAATGGCAACAAGCGACGGTTCTGCCTGAAGCCTTAGTCGAAGCGCAATCCCTAG
CTGGCTCCAAATGTGAACACGCTTGGCGTAGCCAACGCAAAAATAACGATTGGACAGGTTTTGAGAAAAACTGGGCGGAA
GTGGTCAAGCTTTCTCAAGAAGAAGCGCAAATTCGTGCCGAAGCAACTGGGAAAACCCCTTACGATGCGATGCTTGAGCT
TTATGAGCCGGGCACGACAACCGAACAGCTCAATCGTGTATTTAGTGATGTGAAAACTTGGCTACCAAGCTTAATTGATG
TGGTGATTGAAAAGCAGAGTAGCGAAAGCTTTATCGCACCTAAAGGCCATTATCCTGCTGAAAGCCAAAAAGCGCTGGGG
CTGGATGTGATGAAGCTACTTCAGTTTGATTTTAACCACGGCCGACTTGATGAAAGTGTGCACCCCTTCTGTGGCGGCGT
ACCGAGTGATGTGCGCATTACTACACGCTACAACGAAAGCGAGTTTGTACAATCGCTGATGGGCATTGTGCATGAAACTG
GCCATGCGCGTTATGAGCAAGGTTTACCTAAGCATTTGGCCGGCACTCCGGCTGGCGAAGCGCGTTCGATGGGCATTCAT
GAATCACAATCTCTGTTCTTTGAGATGCAAGTGGGTCGTAGCCCTGCGTTTATTGCCCATCTCGCTGAACTGGCAGGCAA
ACATTTTTCGGCGATGAATGATCCGGTCTTCCGAGTGGAAAACATTCAAAAACTTTACACGCGCGTGCAGAAAGATTTTA
TCCGCGTCGATGCGGATGAACTCACCTACCCAGCCCATGTGATCCTGCGTTTTGAAATCGAACGAGATTTGATGAACGGC
AAGATCAAACACACTGATGTGCCAGAACTTTGGGATCAAAAGATGCAGGCTTATTTAGGCCTAAGCACCAAAGGCAACGA
TCAAAATGGGTGTATGCAGGATATTCACTGGACCGATGGCAGCTTTGGTTACTTCCCAAGCTACACCTTAGGTGCCATGT
ATGCGGCGCAATTTATGGCGGCCATGAAGAAAACCGTGGATGTGGATGGCGCGATTCGCCGCGGCGATCTCTCACCGATT
TTTACTTGGTTGTCTGAGAATATTTGGAGTAAGGGCAGCTTATTCAGTACCGATGAATTGGTTAAACAAGCGACGGGGGA
AACCCTCAATCCAGAGCATTTTAAAGCTCATTTGAGTCAGCGCTATCTGAAGTAA

Upstream 100 bases:

>100_bases
CACACCATTCAAAATCAATAAGTTATAGAAATAGTCCCATCAACAAAGCAAAAAACTGCCCAACGCTGAGCAAGATCTTG
CTTGGCGGGCAAAAATCCTC

Downstream 100 bases:

>100_bases
TCTTTCACGAACCCCACGAAATGTGGGGTTCTTTATTTCCTCAAAATCTCACGTCTTCACTGTCATTTTTCAGCGCAAAC
TAGATAATTTTTGTAAATGA

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVH
IHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAE
VVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSMGIH
ESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNG
KIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
FTWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK

Sequences:

>Translated_524_residues
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVH
IHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAE
VVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSMGIH
ESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNG
KIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
FTWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK
>Mature_524_residues
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVH
IHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAE
VVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSMGIH
ESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNG
KIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
FTWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 59210; Mature: 59210

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAA
CCCCCCCCCEEHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCE
VMPSGGAEARSQAMAELSVHIHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQA
ECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAEVVKLSQEEAQIRAEATGKTP
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
YDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQ
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHC
GLPKHLAGTPAGEARSMGIHESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVE
CCCHHHCCCCCCCHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
NIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNGKIKHTDVPELWDQKMQAYLG
HHHHHHHHHHHHHHHCCHHHHCCCEEEEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHC
LSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FTWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKKRASHHLVICTLIDTLRFRLSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAA
CCCCCCCCCEEHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCE
VMPSGGAEARSQAMAELSVHIHGLMTQPQLADWFAEAESETLNIEQQATLREMKRQWQQA
ECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
TVLPEALVEAQSLAGSKCEHAWRSQRKNNDWTGFEKNWAEVVKLSQEEAQIRAEATGKTP
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
YDAMLELYEPGTTTEQLNRVFSDVKTWLPSLIDVVIEKQSSESFIAPKGHYPAESQKALG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHH
LDVMKLLQFDFNHGRLDESVHPFCGGVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQ
HHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHC
GLPKHLAGTPAGEARSMGIHESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVE
CCCHHHCCCCCCCHHHCCCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
NIQKLYTRVQKDFIRVDADELTYPAHVILRFEIERDLMNGKIKHTDVPELWDQKMQAYLG
HHHHHHHHHHHHHHHCCHHHHCCCEEEEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHC
LSTKGNDQNGCMQDIHWTDGSFGYFPSYTLGAMYAAQFMAAMKKTVDVDGAIRRGDLSPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
FTWLSENIWSKGSLFSTDELVKQATGETLNPEHFKAHLSQRYLK
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]