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The map label for this gene is hlyB [H]

Identifier: 227081584

GI number: 227081584

Start: 1463344

End: 1465080

Strand: Reverse

Name: hlyB [H]

Synonym: VCM66_1368

Alternate gene names: 227081584

Gene position: 1465080-1463344 (Counterclockwise)

Preceding gene: 227081585

Following gene: 227081579

Centisome position: 50.65

GC content: 44.73

Gene sequence:

>1737_bases
ATGGCCCAACTCTCGTTTAAAAACAAGATAATTGCGCTGATCATCGCGATTATCTCGCTGACCATCATCACCTCGTATTT
TAGTGTTAACTATTTTATCAGCGATTACATCAAACAGGCGGATAGCCAAAACATCACGCATAACATTGATTTGATGCAAC
GTAAGCTTGAAAACGAACTGAACAGCAAATTAGCTCTCGCACAAAGCCTGAACTTCAGCATGATGGATATTGGTGAAACT
AAAGCGAGCTCTGGATTCAATAAAATCATAAAGATCGTCAACGGATATGCCTTTGATGATACGGGCAATATGAGCGAAGA
AGATGCGCAGCAGTATGTGAGTCTCGCTGAAAATCATGGTGATGCAGTGGTAGTTAGCCCTGTGACATTCAATGAGGGAC
AGCCGACGATTACCTTTTCAGTCAAGCGTTTCGATGAGTCAGTAGATTTCTTCGTGTTCAACCTTTCCGCCTTTAGTCAA
ATGATGACCGATTACGCAACCGAGGGCAGCTTTGCGGAATTGATGGCTAATGGAAATGTGATTTTCTCGGATAAGCATGG
GGTAAACCTCACGCCCATTACTCGTCCAATCAATTTTGCTGGACAAGATTGGCAGTTGATTGGCTACATCGATTTAACCG
CGATTCAAGCCAATACCGATCAGCTCAACTGGAAAATTACCCTCGCGCTTTTAGTCTGTGCCTCCGTCATTATTGTTGCC
AGCGTGACTATGCTGCACGTTTCATTTAAGCCATTGAGCCGCCTTAACACTTTGGTTGCCAACCTATCACAAGGTAATGG
TGATCTAACCCAACGTCTCGCCGTTGAATCAAAAGATGAAATTGGTCAAATCTGCAACTCCATCAACCTTTTCATTGAAA
AACTGCAGCAGATGTTTATCGACGTAGCTGACTCATCGAAAGAGATTGATCGTGCCGTTGTACATTTAAGCAACCAAGCT
CGTTCCAATCTCAATACGCTTAATCAACATACGCAAGAAACTGAACAAGCGATTACCGCCATTGAAGAGATGAGTGCTAG
CGCAGGCTCTATTGCGCAAAGTGCGGATGATGCCGCACTACTTACCGAACGAACAAATCGTTATGCTGATGAATCAAAAC
AAACCGTTACCGAAGCTGTCAACAGTGTCAATGGACTGGTGAACCAAGTCTCCTCGATGGCAGAAACTATCACGCGTATG
AGCGAGGACACGAAGCAAATCAATTCCGTATTACAAGTCATCGGCGCGATTGCAGAACAAACTAATCTACTGGCGCTCAA
TGCGGCGATCGAAGCTGCTCGTGCTGGTGAACAAGGCAGAGGTTTTGCAGTAGTCGCTGATGAAGTTCGTGCATTAGCCT
CTCGGACACAACAGAGCACTTCGCAAATTAATGACATGCTCGCGACCTTAAAAACCACGACAGAAAACGTAGTAAAAGAG
ATGGACTCAACCCGTATTCATTGTGAAGAAACCGCTGAACGCACCAATCATGTGATGGATTCGCTCAATGTGGTGACTGA
TTCAGTCGCTGAAATGAATAATCTGAATACTCTTATCGCCACATCAGCGATGGAGCAGCGGCAAGTTACACACGAAGTGA
GTAAAAACATGGCGGCAATTCAAGAGATGGTGCGTAAGCTCAACATGAATGCCGCGCAAGTCACCTCGGTCAGCAATGAG
TTGCAAAATACCTCACATGCATTGAGTGATGTGGTAGGACGGTTTAGAGTTCAATAA

Upstream 100 bases:

>100_bases
GACACAGCTCACTTAATATGTGACCCGAACTTTTGTATGAGTTTATCAACGGCGCAGGAAATGCCGTTCAAATCGATCAC
TTTTAAGGATGTAAAGTGCT

Downstream 100 bases:

>100_bases
CGCTTTAGTCTTCACCACATTCATAAGCTCGAGAGAATAAAGGCGTAATGCCATGTGTATTACGCCTTTGACGTTTAATG
AACAGCAGAGGTATCCACCG

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MAQLSFKNKIIALIIAIISLTIITSYFSVNYFISDYIKQADSQNITHNIDLMQRKLENELNSKLALAQSLNFSMMDIGET
KASSGFNKIIKIVNGYAFDDTGNMSEEDAQQYVSLAENHGDAVVVSPVTFNEGQPTITFSVKRFDESVDFFVFNLSAFSQ
MMTDYATEGSFAELMANGNVIFSDKHGVNLTPITRPINFAGQDWQLIGYIDLTAIQANTDQLNWKITLALLVCASVIIVA
SVTMLHVSFKPLSRLNTLVANLSQGNGDLTQRLAVESKDEIGQICNSINLFIEKLQQMFIDVADSSKEIDRAVVHLSNQA
RSNLNTLNQHTQETEQAITAIEEMSASAGSIAQSADDAALLTERTNRYADESKQTVTEAVNSVNGLVNQVSSMAETITRM
SEDTKQINSVLQVIGAIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRTQQSTSQINDMLATLKTTTENVVKE
MDSTRIHCEETAERTNHVMDSLNVVTDSVAEMNNLNTLIATSAMEQRQVTHEVSKNMAAIQEMVRKLNMNAAQVTSVSNE
LQNTSHALSDVVGRFRVQ

Sequences:

>Translated_578_residues
MAQLSFKNKIIALIIAIISLTIITSYFSVNYFISDYIKQADSQNITHNIDLMQRKLENELNSKLALAQSLNFSMMDIGET
KASSGFNKIIKIVNGYAFDDTGNMSEEDAQQYVSLAENHGDAVVVSPVTFNEGQPTITFSVKRFDESVDFFVFNLSAFSQ
MMTDYATEGSFAELMANGNVIFSDKHGVNLTPITRPINFAGQDWQLIGYIDLTAIQANTDQLNWKITLALLVCASVIIVA
SVTMLHVSFKPLSRLNTLVANLSQGNGDLTQRLAVESKDEIGQICNSINLFIEKLQQMFIDVADSSKEIDRAVVHLSNQA
RSNLNTLNQHTQETEQAITAIEEMSASAGSIAQSADDAALLTERTNRYADESKQTVTEAVNSVNGLVNQVSSMAETITRM
SEDTKQINSVLQVIGAIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRTQQSTSQINDMLATLKTTTENVVKE
MDSTRIHCEETAERTNHVMDSLNVVTDSVAEMNNLNTLIATSAMEQRQVTHEVSKNMAAIQEMVRKLNMNAAQVTSVSNE
LQNTSHALSDVVGRFRVQ
>Mature_577_residues
AQLSFKNKIIALIIAIISLTIITSYFSVNYFISDYIKQADSQNITHNIDLMQRKLENELNSKLALAQSLNFSMMDIGETK
ASSGFNKIIKIVNGYAFDDTGNMSEEDAQQYVSLAENHGDAVVVSPVTFNEGQPTITFSVKRFDESVDFFVFNLSAFSQM
MTDYATEGSFAELMANGNVIFSDKHGVNLTPITRPINFAGQDWQLIGYIDLTAIQANTDQLNWKITLALLVCASVIIVAS
VTMLHVSFKPLSRLNTLVANLSQGNGDLTQRLAVESKDEIGQICNSINLFIEKLQQMFIDVADSSKEIDRAVVHLSNQAR
SNLNTLNQHTQETEQAITAIEEMSASAGSIAQSADDAALLTERTNRYADESKQTVTEAVNSVNGLVNQVSSMAETITRMS
EDTKQINSVLQVIGAIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRALASRTQQSTSQINDMLATLKTTTENVVKEM
DSTRIHCEETAERTNHVMDSLNVVTDSVAEMNNLNTLIATSAMEQRQVTHEVSKNMAAIQEMVRKLNMNAAQVTSVSNEL
QNTSHALSDVVGRFRVQ

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788195, Length=308, Percent_Identity=30.8441558441558, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788194, Length=397, Percent_Identity=29.7229219143577, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI2367378, Length=366, Percent_Identity=27.5956284153005, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787690, Length=366, Percent_Identity=28.6885245901639, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1789453, Length=326, Percent_Identity=27.3006134969325, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 63400; Mature: 63269

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLSFKNKIIALIIAIISLTIITSYFSVNYFISDYIKQADSQNITHNIDLMQRKLENEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
NSKLALAQSLNFSMMDIGETKASSGFNKIIKIVNGYAFDDTGNMSEEDAQQYVSLAENHG
HHHHHHHHHCCCHHEECCCHHHCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCC
DAVVVSPVTFNEGQPTITFSVKRFDESVDFFVFNLSAFSQMMTDYATEGSFAELMANGNV
CEEEEEEEEECCCCCEEEEEHHHHCCCHHEEEEEHHHHHHHHHHHCCCCCHHHHHCCCCE
IFSDKHGVNLTPITRPINFAGQDWQLIGYIDLTAIQANTDQLNWKITLALLVCASVIIVA
EEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SVTMLHVSFKPLSRLNTLVANLSQGNGDLTQRLAVESKDEIGQICNSINLFIEKLQQMFI
HHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DVADSSKEIDRAVVHLSNQARSNLNTLNQHTQETEQAITAIEEMSASAGSIAQSADDAAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHH
LTERTNRYADESKQTVTEAVNSVNGLVNQVSSMAETITRMSEDTKQINSVLQVIGAIAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TNLLALNAAIEAARAGEQGRGFAVVADEVRALASRTQQSTSQINDMLATLKTTTENVVKE
CCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDSTRIHCEETAERTNHVMDSLNVVTDSVAEMNNLNTLIATSAMEQRQVTHEVSKNMAAI
HHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QEMVRKLNMNAAQVTSVSNELQNTSHALSDVVGRFRVQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQLSFKNKIIALIIAIISLTIITSYFSVNYFISDYIKQADSQNITHNIDLMQRKLENEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
NSKLALAQSLNFSMMDIGETKASSGFNKIIKIVNGYAFDDTGNMSEEDAQQYVSLAENHG
HHHHHHHHHCCCHHEECCCHHHCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCC
DAVVVSPVTFNEGQPTITFSVKRFDESVDFFVFNLSAFSQMMTDYATEGSFAELMANGNV
CEEEEEEEEECCCCCEEEEEHHHHCCCHHEEEEEHHHHHHHHHHHCCCCCHHHHHCCCCE
IFSDKHGVNLTPITRPINFAGQDWQLIGYIDLTAIQANTDQLNWKITLALLVCASVIIVA
EEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
SVTMLHVSFKPLSRLNTLVANLSQGNGDLTQRLAVESKDEIGQICNSINLFIEKLQQMFI
HHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DVADSSKEIDRAVVHLSNQARSNLNTLNQHTQETEQAITAIEEMSASAGSIAQSADDAAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHH
LTERTNRYADESKQTVTEAVNSVNGLVNQVSSMAETITRMSEDTKQINSVLQVIGAIAEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TNLLALNAAIEAARAGEQGRGFAVVADEVRALASRTQQSTSQINDMLATLKTTTENVVKE
CCEEEHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDSTRIHCEETAERTNHVMDSLNVVTDSVAEMNNLNTLIATSAMEQRQVTHEVSKNMAAI
HHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
QEMVRKLNMNAAQVTSVSNELQNTSHALSDVVGRFRVQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]