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The map label for this gene is rpfG [H]

Identifier: 227081519

GI number: 227081519

Start: 1392793

End: 1394118

Strand: Reverse

Name: rpfG [H]

Synonym: VCM66_1303

Alternate gene names: 227081519

Gene position: 1394118-1392793 (Counterclockwise)

Preceding gene: 227081521

Following gene: 227081514

Centisome position: 48.2

GC content: 45.85

Gene sequence:

>1326_bases
ATGGCAACCGCCAATATCGCCATCAAGCAAAACAGTAGTACGAACATCGCTTTTATCGAGCGCATGCCATCTCCTTCTCT
ACACAGGATTTTGTGTAACCTATTTTCAAAGTTTAGCTATAGTTTAAGCAACTCATGTCACTTTAATCTTAAATACCTAA
ACTTTGTGATGAATAGGATGTTTCACATGCCAATGGAAGACCTTAGCCAATGCACGATACTGATCGTCGATGACTCGCCA
GACAATATCGCCTTTATGTCGCAAGGGCTGGCACAGTACTATCGAATCAAGGCGGCTCGCTCAGGAAAAGTGGCACTCGA
GATTTTGGCGCAATACCCTATCGATCTGGTTTTACTCGATATTGTCATGCCAGAAATGTCGGGCTATGAGGTAATTAATC
AAATCAAACATAACCCGCATACCGAGCATATTCCGGTGATTTTTCTTACAGGTAAAAGTAATCCCGAAGATGAGCAATTA
GGCTTTGAGCTTGGTGCCGTTGATTACGTTTTTAAACCGGTCAGCATTCCGCTGCTGAAATCGCGCGTTCACACTCACTT
ACAAAATAAGCGCTCTAAAGACATTTTATTGAATCAAAACGATTATCTGGAAACCGAAGTGATACGACGGTCCGGAGAAT
TGGATCGCATGCAAGATGCTGTGGTGTTTGCGCTGGCCAGTCTTGCAGAAACACGCGATCCGGAGACAGGTAATCACCTA
CTGCGTACCCAACATTACGTGAAAGTATTAGCCCAGCGTCTCGCCACCACGGACAAATACCGAGATGTACTGAGCCCGAC
CGTGATTGATACTTACTTTAAAGCGGCACCGTTGCACGACATAGGTAAAGTCGGCATTCCCGACAATATTCTGCTCAAAC
CGAGCAAATTGACCCCAGATGAGTTTACCACTATGCGCAATCACGCCTTGCTGGGTAAGCTTGCTCTCGAAAAAGCGGAG
AAACTTTCCGGAGCCTGTACAGCGCTCATCAATGTCGCAAAAGAAATTGCCATGGGTCATCATGAAAAGTGGGACGGTTC
AGGTTATCCACTAGGACTCAAAGGCGACGATATTTCTCTGAGTGCCCGCTTGATGGCTCTCGCCGATGTGTATGACGCTT
TGATTTGCCGCCGCGTTTATAAAGAACCGATGAGTCATGAAGAGGCCAAAGCGATCATTTTACAAGGCCGAGGTTCACAT
TTTGACCCAATGGTGATTGATGCTTTTTTGCTGGAGGAGCAGAACTTCATCGACATTGCACAAAAGTTTGCCGATGAAGA
ATCTGCTATGGTACCGATTCAACGGGGTCAACAAGCATCAGGCTGA

Upstream 100 bases:

>100_bases
TTGTTTTAACTCTTGAGATAGACGATAAAAGTGAAAAAGCTGCTCGCTGTATTGATGGCTCTCTAGCCTAAGATCTGCCA
CTTGCCGAGTCACTTTAAAC

Downstream 100 bases:

>100_bases
TTTTCTGGAGCAGCCTGCGCAAACGCAGGCAAAGCACGTAACCTTGCTGCAATCTGCTGTAAGGTGGGATAACGACTCAT
ATCGAGATCAAATCGCTCAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MATANIAIKQNSSTNIAFIERMPSPSLHRILCNLFSKFSYSLSNSCHFNLKYLNFVMNRMFHMPMEDLSQCTILIVDDSP
DNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLDIVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQL
GFELGAVDYVFKPVSIPLLKSRVHTHLQNKRSKDILLNQNDYLETEVIRRSGELDRMQDAVVFALASLAETRDPETGNHL
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPSKLTPDEFTTMRNHALLGKLALEKAE
KLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDISLSARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSH
FDPMVIDAFLLEEQNFIDIAQKFADEESAMVPIQRGQQASG

Sequences:

>Translated_441_residues
MATANIAIKQNSSTNIAFIERMPSPSLHRILCNLFSKFSYSLSNSCHFNLKYLNFVMNRMFHMPMEDLSQCTILIVDDSP
DNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLDIVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQL
GFELGAVDYVFKPVSIPLLKSRVHTHLQNKRSKDILLNQNDYLETEVIRRSGELDRMQDAVVFALASLAETRDPETGNHL
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPSKLTPDEFTTMRNHALLGKLALEKAE
KLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDISLSARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSH
FDPMVIDAFLLEEQNFIDIAQKFADEESAMVPIQRGQQASG
>Mature_440_residues
ATANIAIKQNSSTNIAFIERMPSPSLHRILCNLFSKFSYSLSNSCHFNLKYLNFVMNRMFHMPMEDLSQCTILIVDDSPD
NIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLDIVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQLG
FELGAVDYVFKPVSIPLLKSRVHTHLQNKRSKDILLNQNDYLETEVIRRSGELDRMQDAVVFALASLAETRDPETGNHLL
RTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPSKLTPDEFTTMRNHALLGKLALEKAEK
LSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDISLSARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSHF
DPMVIDAFLLEEQNFIDIAQKFADEESAMVPIQRGQQASG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=207, Percent_Identity=26.5700483091787, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=31.3559322033898, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788550, Length=184, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693157, Length=124, Percent_Identity=33.0645161290323, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=35.5371900826446, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 49496; Mature: 49364

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATANIAIKQNSSTNIAFIERMPSPSLHRILCNLFSKFSYSLSNSCHFNLKYLNFVMNRM
CCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
FHMPMEDLSQCTILIVDDSPDNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLD
HCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
IVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQLGFELGAVDYVFKPVSIPLLK
HHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCHHHHH
SRVHTHLQNKRSKDILLNQNDYLETEVIRRSGELDRMQDAVVFALASLAETRDPETGNHL
HHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPSKLTPD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCEEECCCCCCCH
EFTTMRNHALLGKLALEKAEKLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSHFDPMVIDAFLLEEQNFIDIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
QKFADEESAMVPIQRGQQASG
HHHCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
ATANIAIKQNSSTNIAFIERMPSPSLHRILCNLFSKFSYSLSNSCHFNLKYLNFVMNRM
CCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
FHMPMEDLSQCTILIVDDSPDNIAFMSQGLAQYYRIKAARSGKVALEILAQYPIDLVLLD
HCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
IVMPEMSGYEVINQIKHNPHTEHIPVIFLTGKSNPEDEQLGFELGAVDYVFKPVSIPLLK
HHHCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCHHHHH
SRVHTHLQNKRSKDILLNQNDYLETEVIRRSGELDRMQDAVVFALASLAETRDPETGNHL
HHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
LRTQHYVKVLAQRLATTDKYRDVLSPTVIDTYFKAAPLHDIGKVGIPDNILLKPSKLTPD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCCCCEEECCCCCCCH
EFTTMRNHALLGKLALEKAEKLSGACTALINVAKEIAMGHHEKWDGSGYPLGLKGDDISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
SARLMALADVYDALICRRVYKEPMSHEEAKAIILQGRGSHFDPMVIDAFLLEEQNFIDIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
QKFADEESAMVPIQRGQQASG
HHHCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]