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The map label for this gene is rstB [H]

Identifier: 227081490

GI number: 227081490

Start: 1359764

End: 1361086

Strand: Reverse

Name: rstB [H]

Synonym: VCM66_1274

Alternate gene names: 227081490

Gene position: 1361086-1359764 (Counterclockwise)

Preceding gene: 227081491

Following gene: 227081489

Centisome position: 47.06

GC content: 46.71

Gene sequence:

>1323_bases
ATGCGCCGCATTTACATCGAGTCACTTGTCTCACTGATCGTGCTGTTTTTTGGCAGCTTGGTCAGCTATAGCTTTATTGT
CTATGAATTAGATACCGACTATGACTACGTACTGGAAGATTATCAGGCAGAAGCCTTACAGACTTTATTAAGTCAAATTC
GTCAGTTAGACAGTCCAGAATCGGCGAAAACCGCACTGCGTGGCTATGCCGAACATATCCACAAAACTGTCGACTTACTA
CCACTCACCGAATTACCACCAGAAGTACAACAGCACTTTTCGAGTGAGGCAGCGCATTCTCCTATTTTCCACGATGATGA
CCGTAATTTATGGCTTCAGTTGGACTCCAACGAGCAAATTTATCTCCTCAAACCCGACAGCCACTCTCCTGTCTATCAAG
CCATAGATCGCGCCGATAACCTATTGTTTGTCTTTATGTTAGGTGGATTTGCTCTCTATTGTATGTTCTTAATTTGGTTT
TTGAGTCGCCGGCTACGTGAGTTGGAGCGGGTCACTCACGATTTCGCAAATGGTAATTTGCAAGCGCGAGCGAGTACTAA
AAGCAGTAAAAGCGTGGGTAAGCTCAACCATAGCTTTAACATGATGGCCGATAAAATCTCTCACCTGATCCTCAGTAACA
AAGCGCTCACCAACGCCATCGCACATGATTTACGCACCCCGATCTTCCGTATCCAGTGGCAAGCTGAAGTGTTAGCCGAA
AGCCAGTTGAATGCAAAACAGCAACAGCAAGTTGCGAGCATTATTGAGGACACGGAGGAAATGGAAACCATGGTGGATGA
ACTGCTGTATTACGCCAAACTAGAACATCCAGAAAGCGACATCCAGAGTCAGTATTTAGAAGTTAATCAGTGGCTTGCCG
ACTTTATTGACGAGCAAAGCCATAAAAGCAGTTTACAAATACAATACCTCCCTTCTCTGCAACCCTTAATGCTGGATGCC
GACCCTCAATTGCTCACAAGGGCATTGGTTAACCTCATCCGTAATGCAGAAAAGTACGCAGGTGATCGTCTACTGATTGA
AGCGAGTACCGTCGACGCTAAGCTCTGCATCGCCATACACGACAATGGACCGGGGATTGAAGAGCAACATTGGCCGCATA
TCTTCGATGCCTTTTACAGCACTGACTCGTCACGCAACAAAGCGCAGTCAGGGTTTGGGCTTGGTCTTGCCATCGTTAAG
CAGATCATGGCGCGTCACCAAGGTGAAGTCACGCTGACCAAGAGTCCATTGGGCGGAGCTTGTTTCTCGCTATGGCTACC
TCTATACCCCAACAAACTGGAGTTGCCAGTAAACAAGCAGTGA

Upstream 100 bases:

>100_bases
GATGACTCTAGCACGCCGAAACGGATCATTACGGTACGCGGTAAGGGATATTTATTTGTGCCTGACACTTGGTAAACCTA
AGTTGATTTAAAGGAAACCT

Downstream 100 bases:

>100_bases
GTTGATCCCCATGAGCATCAATAGACAATGCGATTGGGGTAAACCCTCGCGACCCGCACATGATTTCAGCTTCAATTAGA
AAAGATAAGTAGACAGGGAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRRIYIESLVSLIVLFFGSLVSYSFIVYELDTDYDYVLEDYQAEALQTLLSQIRQLDSPESAKTALRGYAEHIHKTVDLL
PLTELPPEVQQHFSSEAAHSPIFHDDDRNLWLQLDSNEQIYLLKPDSHSPVYQAIDRADNLLFVFMLGGFALYCMFLIWF
LSRRLRELERVTHDFANGNLQARASTKSSKSVGKLNHSFNMMADKISHLILSNKALTNAIAHDLRTPIFRIQWQAEVLAE
SQLNAKQQQQVASIIEDTEEMETMVDELLYYAKLEHPESDIQSQYLEVNQWLADFIDEQSHKSSLQIQYLPSLQPLMLDA
DPQLLTRALVNLIRNAEKYAGDRLLIEASTVDAKLCIAIHDNGPGIEEQHWPHIFDAFYSTDSSRNKAQSGFGLGLAIVK
QIMARHQGEVTLTKSPLGGACFSLWLPLYPNKLELPVNKQ

Sequences:

>Translated_440_residues
MRRIYIESLVSLIVLFFGSLVSYSFIVYELDTDYDYVLEDYQAEALQTLLSQIRQLDSPESAKTALRGYAEHIHKTVDLL
PLTELPPEVQQHFSSEAAHSPIFHDDDRNLWLQLDSNEQIYLLKPDSHSPVYQAIDRADNLLFVFMLGGFALYCMFLIWF
LSRRLRELERVTHDFANGNLQARASTKSSKSVGKLNHSFNMMADKISHLILSNKALTNAIAHDLRTPIFRIQWQAEVLAE
SQLNAKQQQQVASIIEDTEEMETMVDELLYYAKLEHPESDIQSQYLEVNQWLADFIDEQSHKSSLQIQYLPSLQPLMLDA
DPQLLTRALVNLIRNAEKYAGDRLLIEASTVDAKLCIAIHDNGPGIEEQHWPHIFDAFYSTDSSRNKAQSGFGLGLAIVK
QIMARHQGEVTLTKSPLGGACFSLWLPLYPNKLELPVNKQ
>Mature_440_residues
MRRIYIESLVSLIVLFFGSLVSYSFIVYELDTDYDYVLEDYQAEALQTLLSQIRQLDSPESAKTALRGYAEHIHKTVDLL
PLTELPPEVQQHFSSEAAHSPIFHDDDRNLWLQLDSNEQIYLLKPDSHSPVYQAIDRADNLLFVFMLGGFALYCMFLIWF
LSRRLRELERVTHDFANGNLQARASTKSSKSVGKLNHSFNMMADKISHLILSNKALTNAIAHDLRTPIFRIQWQAEVLAE
SQLNAKQQQQVASIIEDTEEMETMVDELLYYAKLEHPESDIQSQYLEVNQWLADFIDEQSHKSSLQIQYLPSLQPLMLDA
DPQLLTRALVNLIRNAEKYAGDRLLIEASTVDAKLCIAIHDNGPGIEEQHWPHIFDAFYSTDSSRNKAQSGFGLGLAIVK
QIMARHQGEVTLTKSPLGGACFSLWLPLYPNKLELPVNKQ

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=266, Percent_Identity=32.7067669172932, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1790346, Length=270, Percent_Identity=28.1481481481481, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786783, Length=271, Percent_Identity=28.7822878228782, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789808, Length=323, Percent_Identity=25.6965944272446, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788393, Length=263, Percent_Identity=27.3764258555133, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786912, Length=223, Percent_Identity=32.2869955156951, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=26.7281105990783, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=26.6968325791855, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790436, Length=327, Percent_Identity=23.5474006116208, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=22.8215767634855, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081816, Length=241, Percent_Identity=25.3112033195021, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=319, Percent_Identity=26.9592476489028, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082128, Length=297, Percent_Identity=22.8956228956229, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=325, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=24.5535714285714, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI48994928, Length=214, Percent_Identity=25.7009345794392, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=24.4343891402715, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788279, Length=202, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=25.7510729613734, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50211; Mature: 50211

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIYIESLVSLIVLFFGSLVSYSFIVYELDTDYDYVLEDYQAEALQTLLSQIRQLDSPE
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
SAKTALRGYAEHIHKTVDLLPLTELPPEVQQHFSSEAAHSPIFHDDDRNLWLQLDSNEQI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCEE
YLLKPDSHSPVYQAIDRADNLLFVFMLGGFALYCMFLIWFLSRRLRELERVTHDFANGNL
EEECCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
QARASTKSSKSVGKLNHSFNMMADKISHLILSNKALTNAIAHDLRTPIFRIQWQAEVLAE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEHHHHHHHH
SQLNAKQQQQVASIIEDTEEMETMVDELLYYAKLEHPESDIQSQYLEVNQWLADFIDEQS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
HKSSLQIQYLPSLQPLMLDADPQLLTRALVNLIRNAEKYAGDRLLIEASTVDAKLCIAIH
CCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEE
DNGPGIEEQHWPHIFDAFYSTDSSRNKAQSGFGLGLAIVKQIMARHQGEVTLTKSPLGGA
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCHH
CFSLWLPLYPNKLELPVNKQ
HHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRIYIESLVSLIVLFFGSLVSYSFIVYELDTDYDYVLEDYQAEALQTLLSQIRQLDSPE
CCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
SAKTALRGYAEHIHKTVDLLPLTELPPEVQQHFSSEAAHSPIFHDDDRNLWLQLDSNEQI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCEE
YLLKPDSHSPVYQAIDRADNLLFVFMLGGFALYCMFLIWFLSRRLRELERVTHDFANGNL
EEECCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
QARASTKSSKSVGKLNHSFNMMADKISHLILSNKALTNAIAHDLRTPIFRIQWQAEVLAE
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEHHHHHHHH
SQLNAKQQQQVASIIEDTEEMETMVDELLYYAKLEHPESDIQSQYLEVNQWLADFIDEQS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
HKSSLQIQYLPSLQPLMLDADPQLLTRALVNLIRNAEKYAGDRLLIEASTVDAKLCIAIH
CCCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEE
DNGPGIEEQHWPHIFDAFYSTDSSRNKAQSGFGLGLAIVKQIMARHQGEVTLTKSPLGGA
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCHH
CFSLWLPLYPNKLELPVNKQ
HHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]