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The map label for this gene is rstA [H]

Identifier: 227081491

GI number: 227081491

Start: 1361112

End: 1361816

Strand: Reverse

Name: rstA [H]

Synonym: VCM66_1275

Alternate gene names: 227081491

Gene position: 1361816-1361112 (Counterclockwise)

Preceding gene: 227081493

Following gene: 227081490

Centisome position: 47.08

GC content: 47.09

Gene sequence:

>705_bases
ATGTCTAACCAACCCAGTCTCTACATTATCGAAGACGACACTAAACTTCGCGAAATGTTGGCCGAGTACATGACCAATCA
AGGTTTCCAAGTAACCACTTTTGCGACTGGCGAAACCGCTCCCGAACAAATTCTGCTTAATCAACCTGATCTGGTTCTGC
TCGATCTTATGCTACCTGGCGAAAATGGCCTGACAATTTGTAGACAGATCCGTGCACAATTTCTCGGTAAGATTCTCATG
CTTACCGCCAGTGATGATGATTTTGACCATGTTGCGGCTCTTGAAATGGGCGCCGATGACTTCGTCAACAAGCCAATAAA
ACCCAGAGTGCTTTTGGCGCGGATCCGCATGCTGATGCGCCGAGAAGAGCGAACCTCCGCCAGTGCCGATGCTACCCATT
TATTGCAATTTGGCGGTCTTTTACTTAACCAAAGCCGACGCCACTGCGAATTGGATGGAGAAGTGATCAACTTATCGGAT
AGTGAATTCGATCTGCTGTGGCTCTTAGCTTCAGCGGCGGTTCAAGTTGTATCACGCGAGTTTTTGACCAAATCACTTCG
GGGGATTGAGTATGATGGGCTAGATCGGACAGTCGACAACAAAATTGTCACTTTACGTAAAAAGCTATGTGATGACTCTA
GCACGCCGAAACGGATCATTACGGTACGCGGTAAGGGATATTTATTTGTGCCTGACACTTGGTAA

Upstream 100 bases:

>100_bases
TCTGACAGAAACAAACAGAAATATTCGTTTTACTGATGATATTTTCAGTTAGTATTGGACGTTTGTTCCTTGATGGATAA
ACCGTAAATACGAGGTCCCC

Downstream 100 bases:

>100_bases
ACCTAAGTTGATTTAAAGGAAACCTATGCGCCGCATTTACATCGAGTCACTTGTCTCACTGATCGTGCTGTTTTTTGGCA
GCTTGGTCAGCTATAGCTTT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSNQPSLYIIEDDTKLREMLAEYMTNQGFQVTTFATGETAPEQILLNQPDLVLLDLMLPGENGLTICRQIRAQFLGKILM
LTASDDDFDHVAALEMGADDFVNKPIKPRVLLARIRMLMRREERTSASADATHLLQFGGLLLNQSRRHCELDGEVINLSD
SEFDLLWLLASAAVQVVSREFLTKSLRGIEYDGLDRTVDNKIVTLRKKLCDDSSTPKRIITVRGKGYLFVPDTW

Sequences:

>Translated_234_residues
MSNQPSLYIIEDDTKLREMLAEYMTNQGFQVTTFATGETAPEQILLNQPDLVLLDLMLPGENGLTICRQIRAQFLGKILM
LTASDDDFDHVAALEMGADDFVNKPIKPRVLLARIRMLMRREERTSASADATHLLQFGGLLLNQSRRHCELDGEVINLSD
SEFDLLWLLASAAVQVVSREFLTKSLRGIEYDGLDRTVDNKIVTLRKKLCDDSSTPKRIITVRGKGYLFVPDTW
>Mature_233_residues
SNQPSLYIIEDDTKLREMLAEYMTNQGFQVTTFATGETAPEQILLNQPDLVLLDLMLPGENGLTICRQIRAQFLGKILML
TASDDDFDHVAALEMGADDFVNKPIKPRVLLARIRMLMRREERTSASADATHLLQFGGLLLNQSRRHCELDGEVINLSDS
EFDLLWLLASAAVQVVSREFLTKSLRGIEYDGLDRTVDNKIVTLRKKLCDDSSTPKRIITVRGKGYLFVPDTW

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=45.3389830508475, Blast_Score=193, Evalue=6e-51,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=39.1489361702128, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=36.5638766519824, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=34.7639484978541, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787229, Length=231, Percent_Identity=35.0649350649351, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=32.7433628318584, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1790863, Length=239, Percent_Identity=34.3096234309623, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=32.5892857142857, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=33.1838565022422, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=34.061135371179, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=34.6666666666667, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=32.7354260089686, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=32.8828828828829, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=28.8888888888889, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790299, Length=101, Percent_Identity=29.7029702970297, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26434; Mature: 26303

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQPSLYIIEDDTKLREMLAEYMTNQGFQVTTFATGETAPEQILLNQPDLVLLDLMLPG
CCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCEEEEEEEECC
ENGLTICRQIRAQFLGKILMLTASDDDFDHVAALEMGADDFVNKPIKPRVLLARIRMLMR
CCCHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
REERTSASADATHLLQFGGLLLNQSRRHCELDGEVINLSDSEFDLLWLLASAAVQVVSRE
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHH
FLTKSLRGIEYDGLDRTVDNKIVTLRKKLCDDSSTPKRIITVRGKGYLFVPDTW
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCC
>Mature Secondary Structure 
SNQPSLYIIEDDTKLREMLAEYMTNQGFQVTTFATGETAPEQILLNQPDLVLLDLMLPG
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCEEEEEEEECC
ENGLTICRQIRAQFLGKILMLTASDDDFDHVAALEMGADDFVNKPIKPRVLLARIRMLMR
CCCHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHH
REERTSASADATHLLQFGGLLLNQSRRHCELDGEVINLSDSEFDLLWLLASAAVQVVSRE
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHH
FLTKSLRGIEYDGLDRTVDNKIVTLRKKLCDDSSTPKRIITVRGKGYLFVPDTW
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]