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The map label for this gene is asnC [H]

Identifier: 227081468

GI number: 227081468

Start: 1333433

End: 1334878

Strand: Reverse

Name: asnC [H]

Synonym: VCM66_1252

Alternate gene names: 227081468

Gene position: 1334878-1333433 (Counterclockwise)

Preceding gene: 227081469

Following gene: 227081466

Centisome position: 46.15

GC content: 49.38

Gene sequence:

>1446_bases
ATGGCATTGGGATGTCTTTATATCTCAACGGAGACTGTAATAAACATGACTTACGCGCCTGTAAATGACGTACTCAGCGG
CAAGCTCGCAGTAGACAGTGAAGTCACTGTTCGCGGCTGGATTCGCACACGTCGTGATTCCAAAGCTGGAATTTCGTTTC
TTGCCATCTATGACGGCTCTTGTTTCAACCCGATTCAGGCCGTGGTTCCTAATAATCTCAATAATTACGACAACGAAGTT
CTGAAGCTCACTACAGGCTGTTCCGTTGAAGTAACCGGTAAGATTGTTGAATCTCCAGCTTCAGGCCAAGCATTCGAACT
GGCTGCGTCTGACGTAAAAGTCGTCGGTTGGGTTGAAGATGCCGATACTTACCCAATGGCGAAAACGCGTCATTCGATTG
AATATCTGCGTGAAGTGGCTCACCTGCGTCCACGTACTAACGTGATCGGTGCGGTTGCGCGTGTTCGTAACTGCTTGGCA
CAAGCGATTCACCGTTTCTACCATGAACAAGGTTACTTCTGGGTTTCAGCGCCTCTTATCACCGCTTCTGATGCAGAAGG
CGCAGGTGAAATGTTCCGCGTTTCAACGCTGGATATGGAAAACCTACCGCGTACTGACGCAGGTAAAGTGGATTACAACC
AAGACTTCTTTGGTAAAGAAACCTTCCTGACCGTATCAGGCCAGCTCAACGCGGAAGCTTACGCTTGTGCGATCAGCAAA
GTGTACACTTTCGGCCCAACATTCCGTGCGGAAAACTCAAACACCAGCCGTCACTTGGCGGAATTCTGGATGGTTGAACC
TGAAGTTGCATTCGCTGACCTGAACACTGTAGCAAAGCTGGCGGAAGACATGCTGAAGTACGTGTTCAAAGCCGTACTGG
CAGAGCGTCGTGACGATCTTGAGTTCTTTAACGATCGTATTAATAACGAAGTGATTGCGCGTCTTGAGCAATTTGTTGAA
TCTGATTTCGCACAAGTGGATTACACTGATGCGATTGAAATTCTGAAAAACTGCGGCAAAACGTTTGAGTTCCCTGTTGA
ATGGGGCATTGATTTAGCTTCTGAGCACGAGCGTTTCCTTGCAGAAGAGCACTTCAAAGCACCGGTTATCGTGAAGAACT
ATCCAAAAGACATCAAAGCGTTTTACATGCGTATGAACGAAGATGGCAAAACCGTTGCAGCGATGGACGTCTTGGCACCG
GGCATTGGTGAAATCATCGGCGGTTCACAGCGTGAAGAGCGTTTGGATATTCTTGATGCGCGTATGCGTGAATTTGGCAT
CGATCCTGAGCACATGGACTGGTATCGCGACCTGCGCCGTTACGGCACAGTGCCGCACGCGGGCTTTGGTCTGGGCTTCG
AGCGTCTAGTCTCTTACGTCACTGGTATGGGCAACGTGCGTGACGTGATCCCATTCCCACGTACCCCACGCAGCGCGAGT
TTCTAA

Upstream 100 bases:

>100_bases
GGTTGAAGCTGACAAATTGACAGGTAATATGTTCCGTTGAATCTGTAAAACTCTCTACAACCCAATGTTTCGTCTATTTT
TTGCGCGATGACGAGCGCAA

Downstream 100 bases:

>100_bases
TTCCGCTCTGTTGAATGTAAAAACCGTCTCAAGCGAGACGGTTTTTTTATAACTCAAGCTCACTCTTATTGGCTTTTCTG
CTGGCTTTTAGCCATTTGCA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MALGCLYISTETVINMTYAPVNDVLSGKLAVDSEVTVRGWIRTRRDSKAGISFLAIYDGSCFNPIQAVVPNNLNNYDNEV
LKLTTGCSVEVTGKIVESPASGQAFELAASDVKVVGWVEDADTYPMAKTRHSIEYLREVAHLRPRTNVIGAVARVRNCLA
QAIHRFYHEQGYFWVSAPLITASDAEGAGEMFRVSTLDMENLPRTDAGKVDYNQDFFGKETFLTVSGQLNAEAYACAISK
VYTFGPTFRAENSNTSRHLAEFWMVEPEVAFADLNTVAKLAEDMLKYVFKAVLAERRDDLEFFNDRINNEVIARLEQFVE
SDFAQVDYTDAIEILKNCGKTFEFPVEWGIDLASEHERFLAEEHFKAPVIVKNYPKDIKAFYMRMNEDGKTVAAMDVLAP
GIGEIIGGSQREERLDILDARMREFGIDPEHMDWYRDLRRYGTVPHAGFGLGFERLVSYVTGMGNVRDVIPFPRTPRSAS
F

Sequences:

>Translated_481_residues
MALGCLYISTETVINMTYAPVNDVLSGKLAVDSEVTVRGWIRTRRDSKAGISFLAIYDGSCFNPIQAVVPNNLNNYDNEV
LKLTTGCSVEVTGKIVESPASGQAFELAASDVKVVGWVEDADTYPMAKTRHSIEYLREVAHLRPRTNVIGAVARVRNCLA
QAIHRFYHEQGYFWVSAPLITASDAEGAGEMFRVSTLDMENLPRTDAGKVDYNQDFFGKETFLTVSGQLNAEAYACAISK
VYTFGPTFRAENSNTSRHLAEFWMVEPEVAFADLNTVAKLAEDMLKYVFKAVLAERRDDLEFFNDRINNEVIARLEQFVE
SDFAQVDYTDAIEILKNCGKTFEFPVEWGIDLASEHERFLAEEHFKAPVIVKNYPKDIKAFYMRMNEDGKTVAAMDVLAP
GIGEIIGGSQREERLDILDARMREFGIDPEHMDWYRDLRRYGTVPHAGFGLGFERLVSYVTGMGNVRDVIPFPRTPRSAS
F
>Mature_480_residues
ALGCLYISTETVINMTYAPVNDVLSGKLAVDSEVTVRGWIRTRRDSKAGISFLAIYDGSCFNPIQAVVPNNLNNYDNEVL
KLTTGCSVEVTGKIVESPASGQAFELAASDVKVVGWVEDADTYPMAKTRHSIEYLREVAHLRPRTNVIGAVARVRNCLAQ
AIHRFYHEQGYFWVSAPLITASDAEGAGEMFRVSTLDMENLPRTDAGKVDYNQDFFGKETFLTVSGQLNAEAYACAISKV
YTFGPTFRAENSNTSRHLAEFWMVEPEVAFADLNTVAKLAEDMLKYVFKAVLAERRDDLEFFNDRINNEVIARLEQFVES
DFAQVDYTDAIEILKNCGKTFEFPVEWGIDLASEHERFLAEEHFKAPVIVKNYPKDIKAFYMRMNEDGKTVAAMDVLAPG
IGEIIGGSQREERLDILDARMREFGIDPEHMDWYRDLRRYGTVPHAGFGLGFERLVSYVTGMGNVRDVIPFPRTPRSASF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=463, Percent_Identity=42.548596112311, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI4758762, Length=451, Percent_Identity=29.490022172949, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI45439306, Length=342, Percent_Identity=28.9473684210526, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1787161, Length=466, Percent_Identity=77.8969957081545, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=369, Percent_Identity=22.4932249322493, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71984122, Length=456, Percent_Identity=29.1666666666667, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17556372, Length=429, Percent_Identity=29.8368298368298, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17551876, Length=482, Percent_Identity=27.5933609958506, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319872, Length=468, Percent_Identity=38.2478632478632, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6321807, Length=460, Percent_Identity=31.0869565217391, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323011, Length=488, Percent_Identity=25.2049180327869, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922416, Length=452, Percent_Identity=36.283185840708, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI19921528, Length=460, Percent_Identity=30.2173913043478, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI17136276, Length=492, Percent_Identity=26.4227642276423, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 54066; Mature: 53935

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGCLYISTETVINMTYAPVNDVLSGKLAVDSEVTVRGWIRTRRDSKAGISFLAIYDGS
CEEEEEEEECCCEEEEEECCHHHHHCCCEEECCCEEEEHHHHHCCCCCCCEEEEEEECCC
CFNPIQAVVPNNLNNYDNEVLKLTTGCSVEVTGKIVESPASGQAFELAASDVKVVGWVED
CCCCHHHHCCCCCCCCCCCEEEEECCCCEEEECCEECCCCCCCEEEEECCCEEEEEEEEC
ADTYPMAKTRHSIEYLREVAHLRPRTNVIGAVARVRNCLAQAIHRFYHEQGYFWVSAPLI
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
TASDAEGAGEMFRVSTLDMENLPRTDAGKVDYNQDFFGKETFLTVSGQLNAEAYACAISK
EECCCCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
VYTFGPTFRAENSNTSRHLAEFWMVEPEVAFADLNTVAKLAEDMLKYVFKAVLAERRDDL
HHCCCCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
EFFNDRINNEVIARLEQFVESDFAQVDYTDAIEILKNCGKTFEFPVEWGIDLASEHERFL
HHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHH
AEEHFKAPVIVKNYPKDIKAFYMRMNEDGKTVAAMDVLAPGIGEIIGGSQREERLDILDA
HHHHCCCCEEECCCCHHHHHHHHHCCCCCCEEEHHHHHCCCHHHHHCCCCHHHHHHHHHH
RMREFGIDPEHMDWYRDLRRYGTVPHAGFGLGFERLVSYVTGMGNVRDVIPFPRTPRSAS
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
F
C
>Mature Secondary Structure 
ALGCLYISTETVINMTYAPVNDVLSGKLAVDSEVTVRGWIRTRRDSKAGISFLAIYDGS
EEEEEEEECCCEEEEEECCHHHHHCCCEEECCCEEEEHHHHHCCCCCCCEEEEEEECCC
CFNPIQAVVPNNLNNYDNEVLKLTTGCSVEVTGKIVESPASGQAFELAASDVKVVGWVED
CCCCHHHHCCCCCCCCCCCEEEEECCCCEEEECCEECCCCCCCEEEEECCCEEEEEEEEC
ADTYPMAKTRHSIEYLREVAHLRPRTNVIGAVARVRNCLAQAIHRFYHEQGYFWVSAPLI
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
TASDAEGAGEMFRVSTLDMENLPRTDAGKVDYNQDFFGKETFLTVSGQLNAEAYACAISK
EECCCCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
VYTFGPTFRAENSNTSRHLAEFWMVEPEVAFADLNTVAKLAEDMLKYVFKAVLAERRDDL
HHCCCCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
EFFNDRINNEVIARLEQFVESDFAQVDYTDAIEILKNCGKTFEFPVEWGIDLASEHERFL
HHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCHHCCCCHHHHHHHHH
AEEHFKAPVIVKNYPKDIKAFYMRMNEDGKTVAAMDVLAPGIGEIIGGSQREERLDILDA
HHHHCCCCEEECCCCHHHHHHHHHCCCCCCEEEHHHHHCCCHHHHHCCCCHHHHHHHHHH
RMREFGIDPEHMDWYRDLRRYGTVPHAGFGLGFERLVSYVTGMGNVRDVIPFPRTPRSAS
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]