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The map label for this gene is rpfG [H]

Identifier: 227081466

GI number: 227081466

Start: 1330532

End: 1332010

Strand: Reverse

Name: rpfG [H]

Synonym: VCM66_1250

Alternate gene names: 227081466

Gene position: 1332010-1330532 (Counterclockwise)

Preceding gene: 227081468

Following gene: 227081465

Centisome position: 46.05

GC content: 48.34

Gene sequence:

>1479_bases
TTGACCATTTGGGTTCTATCACTTGCTATGAAAAACACGATTTACCATGCCCCACTGACGTTTGGACTCTATGCGCTTGC
TGGAGTGCTCTTTGCGCTTTACACAAGCCGAGTCTGTCCTTTTCTCGCGACATTAAGCACTCGTGAAATCGCCACTCAGG
TGGGTGCTGTCTTCATCCTCGCATGGCTGATTCGGCATACTCTGCTTCGCCAGCACATTTGGGCAAGAAAACAGCAATTT
ATCCAATTGGACACGGCACTCCTCTTCGCAATGAGCCTGCCTTTAGCGCTTTACTACAATCTGCAATACCAGTTCACGTT
AGACAGTAATTTAAAAGTACTGTTTGGAATGACCTTATTTGGTTTCTTCACCGGAGCGTTATTGCAATTAAGCAGTAAGT
TAAGAACGCTGCGCCAAATGCCTCAAAGTCAGAACCTTGCTCTCTCTTCTGATGAGCGACGTTCCTTAGTCAAACAGCTG
ATTGGCTTGATCGTCCTTTTAATCAGCACACTGACAGTGATGCTCAGCATGGTCGCAATCAAAGATATTCACTGGCTGGA
AAATAACCCTGCGCGCCTACTTGATGGCAGTGGCAAGATCAGCATTGTGAAAGAGTTTGCCTTTTTGTCCTTGGTGTTAG
GCGGCTACATCACGGCCATTCTCGTACTCTGGAGTCGAATGATGAAAGAGATCCTCGACCATCAGGAGCGCTCATTGCAA
GCCGTGACGCAAGGCAACCTCCAAGTACGCCTTCCCGTCTACTCTAACGATGAGTTAGGCAATGTCGCCATGTTGACCAA
TCAGATGTTAGACAGCTTAGAAGCTACGCAAAATGAGGTCAAAACCACACGTGATGTGGCTATCGTCAGCTTATCGGCTC
TTGCGGAATCACGAGATAACGAGACAGGGGCACATATTCTTCGCACTCAAGAATACGTCAAAGCGTTAGCAGAATATCTC
GCTGCTTTTCCCCAATATTCAACGCTGTTAACCCCGGCTTATATCGAACTACTCTACAAATCCGCGCCATTGCATGATGT
AGGCAAGGTTGGCATTCCCGATTCGGTTCTGCTCAAACCGGGCAAACTGACCGATGAGGAGTTCACCGTCATGAAAGAGC
ATCCGCGTATCGGGGCGCAAGCACTTGCGATTGCTGAGCGCCATTTAGGGACGAGCTCCTTCCTCGCCATTGCCAAAGAA
ATTGCTCTCACTCACCACGAAAAATGGGATGGCACGGGTTATCCGGCGCAATTACAAGGAGAAGCCATCCCGCTTTCCGG
TCGCTTAATGGCGTTAGCCGATGTGTATGATGCCTTGATTTCAGCTCGGGTTTATAAACCGGCTTTTAGTCATGACAAAG
CCAAAGCCATTATTGTCGAAGGCAGTGGTCACCATTTTGATCCTGCCGTGGTCGAGGCTTTTCTCGCGGTTGAGGAAAAG
TTCGTAGCCATTGCAGCACACTTTAAAGATGCCGCATAA

Upstream 100 bases:

>100_bases
ATCACCCAGCAATGTCTATAAAATCCGCGCCTTTATTCAAAATGATAATAAATCACACTCACTTTAATGACGCTGCTTAT
ACTTAACTAGCCGTAACTTA

Downstream 100 bases:

>100_bases
GGTTGTAGTTATACCCTTCCTACTTGAAGCTGCAGCGGTGTTGGCGACGCTCTGCTTTGCGAATGCTCAATCCATGACGA
TGGAAAGAAATGAAAATTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFILAWLIRHTLLRQHIWARKQQF
IQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLFGFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQL
IGLIVLLISTLTVMLSMVAIKDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ
AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDNETGAHILRTQEYVKALAEYL
AAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKE
IALTHHEKWDGTGYPAQLQGEAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK
FVAIAAHFKDAA

Sequences:

>Translated_492_residues
MTIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFILAWLIRHTLLRQHIWARKQQF
IQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLFGFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQL
IGLIVLLISTLTVMLSMVAIKDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ
AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDNETGAHILRTQEYVKALAEYL
AAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKE
IALTHHEKWDGTGYPAQLQGEAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK
FVAIAAHFKDAA
>Mature_491_residues
TIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFILAWLIRHTLLRQHIWARKQQFI
QLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLFGFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQLI
GLIVLLISTLTVMLSMVAIKDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQA
VTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDNETGAHILRTQEYVKALAEYLA
AFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKEI
ALTHHEKWDGTGYPAQLQGEAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEKF
VAIAAHFKDAA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 54566; Mature: 54435

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS50885 HAMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFIL
CEEEEEEHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLIRHTLLRQHIWARKQQFIQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEECCCCHHHHHHHHH
GFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQLIGLIVLLISTLTVMLSMVAI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ
HHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDN
HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ETGAHILRTQEYVKALAEYLAAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKP
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECC
GKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKEIALTHHEKWDGTGYPAQLQG
CCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECC
EAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK
CEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
FVAIAAHFKDAA
HHHHHHHHCCCC
>Mature Secondary Structure 
TIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFIL
EEEEEEHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLIRHTLLRQHIWARKQQFIQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEECCCCHHHHHHHHH
GFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQLIGLIVLLISTLTVMLSMVAI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ
HHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDN
HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ETGAHILRTQEYVKALAEYLAAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKP
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECC
GKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKEIALTHHEKWDGTGYPAQLQG
CCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECC
EAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK
CEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
FVAIAAHFKDAA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]