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The map label for this gene is galR [H]

Identifier: 227081458

GI number: 227081458

Start: 1318656

End: 1319678

Strand: Direct

Name: galR [H]

Synonym: VCM66_1241

Alternate gene names: 227081458

Gene position: 1318656-1319678 (Clockwise)

Preceding gene: 227081457

Following gene: 227081460

Centisome position: 45.59

GC content: 48.97

Gene sequence:

>1023_bases
ATGGCAAGAATTAAAGATGTTGCTGAACTTGCAGGTGTGAACCGCTCTACCGTATCGAGGATCATCAATGGTGAGGGGAA
ATTTAAAGAGGAAACGCGGCGTAAGGTTGAGCAAGCAATGGCTCAACTTAATTATCGGCCGAGTGCAATAGCCCGTTCTT
TGGCGACCTCCTCATCGAATATGATGGGTCTTCTAGTCACGTATTACACAGGTGGCTTTTTTGGTGAAATGATGGAACAG
GTGCAAACTGAGTTGGATATGCACCATAAGTTTTTGATCACAGCACAAGGCCATCACTCTGCACAAGGAGAGAGAGAGGC
GATACAGCGCTTTCACGATCTGCGTTGCGATGGGTATGTTCTGCACAGCCGGTATTTATCAGACGATGATTTGCGAGCAT
TAGCTCAGCAGCCGACTCCCTTTGTTCTTCTCGATCGTTATGTGGCAGGTTTGGAAGAGCGCTGCATTACTTTTGACCAT
CGTGGAGCAAGCCAGATAGTGGTGGAGTATCTCATTCAGCAAGGCCACAGGAAAATTGCTTGTATCACCGGACCGCTACA
CAGACACAATAGTCGTCTACGGCAGCAAGGCTATCTCGATGCCTTAGCTCAAGCGGGGATTCTCCCGAGCAAGTCACTGT
GTGAAGAAGGCAATTATGGTCGCAAAAGTGGTTATTTTGCGATGCAGCAGCTATTCGCTCGCCACCCCGAGATCACGGCT
GTTTTCTCGTGTAGTGAGGAGATGACGACTGGGGCGCTGCAGTATTTACATGAACAAGGTATTGCGGTGCCGGGACGGAT
TTCTATCGTCAGTTTTGACAGTGTTGATTTGTGTGAGAGTCTATATCCAACGGTGACGGCGGTACATTTCCCGATCAGCG
AAATGGCACGTGCGGCGGTGCAGTCATTGATTGCGATGGTGAAACAGCAACCGACAGTCGGTCCGCAAAGCTTTCGTCCG
ATCTTAAAAAAGCGTCACGCCGATCAACCACTGTCGAAAATAGCTGAAAGGCAAGACTCATAA

Upstream 100 bases:

>100_bases
TGGGAGCTGTCTCGCCGAGTGGAGACTTCCGTAAGGGAGTGTCGTGCAAGCTCGCAGTTCCTTGTTTTTCTGAAGTTTCT
GACTTCCTATAGGCTCTAAT

Downstream 100 bases:

>100_bases
ACGCCCCTTAGTGATTTGAAGTTTACAGCCAACGCTAACGCTGCTGTAGCGTTAAGTAATAGGGGAGTAAAAAGTCAGCG
GGGATAAAAAGTAAGAGCGG

Product: transcriptional regulator, LacI family

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MARIKDVAELAGVNRSTVSRIINGEGKFKEETRRKVEQAMAQLNYRPSAIARSLATSSSNMMGLLVTYYTGGFFGEMMEQ
VQTELDMHHKFLITAQGHHSAQGEREAIQRFHDLRCDGYVLHSRYLSDDDLRALAQQPTPFVLLDRYVAGLEERCITFDH
RGASQIVVEYLIQQGHRKIACITGPLHRHNSRLRQQGYLDALAQAGILPSKSLCEEGNYGRKSGYFAMQQLFARHPEITA
VFSCSEEMTTGALQYLHEQGIAVPGRISIVSFDSVDLCESLYPTVTAVHFPISEMARAAVQSLIAMVKQQPTVGPQSFRP
ILKKRHADQPLSKIAERQDS

Sequences:

>Translated_340_residues
MARIKDVAELAGVNRSTVSRIINGEGKFKEETRRKVEQAMAQLNYRPSAIARSLATSSSNMMGLLVTYYTGGFFGEMMEQ
VQTELDMHHKFLITAQGHHSAQGEREAIQRFHDLRCDGYVLHSRYLSDDDLRALAQQPTPFVLLDRYVAGLEERCITFDH
RGASQIVVEYLIQQGHRKIACITGPLHRHNSRLRQQGYLDALAQAGILPSKSLCEEGNYGRKSGYFAMQQLFARHPEITA
VFSCSEEMTTGALQYLHEQGIAVPGRISIVSFDSVDLCESLYPTVTAVHFPISEMARAAVQSLIAMVKQQPTVGPQSFRP
ILKKRHADQPLSKIAERQDS
>Mature_339_residues
ARIKDVAELAGVNRSTVSRIINGEGKFKEETRRKVEQAMAQLNYRPSAIARSLATSSSNMMGLLVTYYTGGFFGEMMEQV
QTELDMHHKFLITAQGHHSAQGEREAIQRFHDLRCDGYVLHSRYLSDDDLRALAQQPTPFVLLDRYVAGLEERCITFDHR
GASQIVVEYLIQQGHRKIACITGPLHRHNSRLRQQGYLDALAQAGILPSKSLCEEGNYGRKSGYFAMQQLFARHPEITAV
FSCSEEMTTGALQYLHEQGIAVPGRISIVSFDSVDLCESLYPTVTAVHFPISEMARAAVQSLIAMVKQQPTVGPQSFRPI
LKKRHADQPLSKIAERQDS

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=37.5757575757576, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788474, Length=343, Percent_Identity=32.0699708454811, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1789068, Length=330, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=32.0121951219512, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=31.1572700296736, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=30.42071197411, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786540, Length=323, Percent_Identity=27.5541795665635, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=25.4658385093168, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790715, Length=307, Percent_Identity=23.7785016286645, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=23.961661341853, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790689, Length=309, Percent_Identity=29.4498381877023, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787906, Length=263, Percent_Identity=28.8973384030418, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789456, Length=303, Percent_Identity=25.4125412541254, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786268, Length=291, Percent_Identity=20.9621993127148, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38111; Mature: 37980

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIKDVAELAGVNRSTVSRIINGEGKFKEETRRKVEQAMAQLNYRPSAIARSLATSSSN
CCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
MMGLLVTYYTGGFFGEMMEQVQTELDMHHKFLITAQGHHSAQGEREAIQRFHDLRCDGYV
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE
LHSRYLSDDDLRALAQQPTPFVLLDRYVAGLEERCITFDHRGASQIVVEYLIQQGHRKIA
EEHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCEEE
CITGPLHRHNSRLRQQGYLDALAQAGILPSKSLCEEGNYGRKSGYFAMQQLFARHPEITA
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEE
VFSCSEEMTTGALQYLHEQGIAVPGRISIVSFDSVDLCESLYPTVTAVHFPISEMARAAV
EEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHH
QSLIAMVKQQPTVGPQSFRPILKKRHADQPLSKIAERQDS
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
ARIKDVAELAGVNRSTVSRIINGEGKFKEETRRKVEQAMAQLNYRPSAIARSLATSSSN
CCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
MMGLLVTYYTGGFFGEMMEQVQTELDMHHKFLITAQGHHSAQGEREAIQRFHDLRCDGYV
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE
LHSRYLSDDDLRALAQQPTPFVLLDRYVAGLEERCITFDHRGASQIVVEYLIQQGHRKIA
EEHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCEEE
CITGPLHRHNSRLRQQGYLDALAQAGILPSKSLCEEGNYGRKSGYFAMQQLFARHPEITA
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEE
VFSCSEEMTTGALQYLHEQGIAVPGRISIVSFDSVDLCESLYPTVTAVHFPISEMARAAV
EEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHH
QSLIAMVKQQPTVGPQSFRPILKKRHADQPLSKIAERQDS
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]