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The map label for this gene is nrdA [H]

Identifier: 227081428

GI number: 227081428

Start: 1283823

End: 1286108

Strand: Reverse

Name: nrdA [H]

Synonym: VCM66_1211

Alternate gene names: 227081428

Gene position: 1286108-1283823 (Counterclockwise)

Preceding gene: 227081429

Following gene: 227081427

Centisome position: 44.46

GC content: 48.78

Gene sequence:

>2286_bases
ATGAACCAACAACTTACTGTCACCAAGCGTGATGGCCGCAAAGAAAACATTGATCTGGATAAGATCCACCGAGTGATCAC
TTGGGCTGCCGAAGGTCTGCAAAACGTATCTGTCTCACAAGTAGAACTGCGCGCTCACATTCAGTTCTATGACGGCATTA
CGACTTCAGACATTCATGAAACCATCATTAAGTCAGCAGCGGATTTGATCTCAGAAGAGACCCCCGACTACCAATACCTT
GCTGCGCGTCTTGCGGTTTTCCACCTGCGCAAAAAGGCCTACGGTCAATACGAGCCACCTACACTGCTCGCTCATGTCAC
CAAGATGGTTGAGAAAGGCAAATACGACAAGCATTTGCTCGAAGACTATACCCGTGAAGAGCTGGAGCTGATGGATCAGT
TCATTGATCACCGTCGTGACCTTGATTTCTCTTACGCTGCAGTGAAACAGCTTGAAGGCAAATATTTCGTACAAAACCGT
GTTACCGGCCAAATCTACGAAAGTGCACAGTTCCTCTATATTCTGGTTGCGGCTTGCCTGTTTGCGAAGTATCCCAAAGA
GACGCGCCTTGATTACATCAAGCGTTTCTACGATGCGACGTCGACCTTTAAGATCTCGCTACCTACACCGATTATGTCGG
GTGTACGTACTCCTACTCGTCAGTTCAGCTCTTGTGTACTGATTGAATGTGGTGACAGCCTTGATTCTATCAATGCAACG
GCAAGCTCGATTGTTCGCTACGTTTCTCAACGTGCCGGTATTGGTATCAACGCAGGCCGCATTCGTGCGCTGGGTTCGGA
AATCCGTGGTGGTGAAGCGTTCCACACTGGCTGTATCCCCTTCTACAAATACTTCCAAACCGCAGTGAAATGCTGCTCGC
AAGGTGGTGTTCGTGGTGGTGCAGCAACCGTATTCTACCCTCTGTGGCATGGTGAAGCGGAATCTCTGCTGGTTCTGAAG
AACAACCGTGGTGTAGAAGAGAACCGTGTTCGTCATATGGATTACGGTGTTCAGCTCAACAAACTGATGTACAAGCGTCT
GGTTGAAGGTGGCAACATTACCCTGTTCTCTCCTTCTGATGTGCCGGGACTGTACGACGCTTTCTTCCAAGATCAGGATG
AGTTCGAACGCCTGTACGTGAAGTACGAAAACGATCCTTCAATTAAGAAGAAAACCGTTAAAGCGCTGGAAATCTTTACT
CTGCTGATGCAAGAGCGTGCTTCAACAGGCCGTATCTACATCCAAAACGTTGACCACTGTAACACGCACAGCCCATTTGA
TGCGGCGGTTGCACCTGTGCGTCAATCAAACCTGTGTCTGGAAATCGCGCTACCGACTTCGCCACTGGCGAATGTGGAAG
ATGAAAGCGGTGAAATTGCTCTGTGTACCCTCTCTGCGTTTAACTTAGGCGCGATTGAGTCACTGGATGATTTTGAAGAG
CTGTCTGACCTTGTGGTTCGTGCTCTAGATGCATTATTGGATTACCAAGACTACCCACTGCCTGCGGCACGTCGTTCTTC
AATGAACCGCCGTACTCTGGGTGTGGGTGTGATCAACTACGCTTACTACCTAGCGAAAAACGGTGTGAAATACTCTGACG
GCAGCGCAAATGGCCTGACGCACCGCACGTTTGAAGCCATGCAGTATCACTTGCTGCGCGCTTCTATGAAGCTGGCGAAA
GAACAAGGTCGTTGCCCACTGTTCCATGAGACCAACTACGCGAAAGGCTTGATGCCAATCGATACTTATAAGAAAGATAT
CGATCTGGTGTGTGCAGAGCCTCTGCATTACGACTGGGATAAGCTGCGTCACGAGATCATGGAACACGGTCTGCGTAACT
CTACGCTGACGGCACTGATGCCTTCTGAGACTTCATCGCAAATCTCTAACGCGACCAACGGTATTGAGCCACCACGTGGT
TACGTCTCGGTCAAAGCCTCAAAAGACGGTATCTTGAAACAAGTGGTTCCAGAATTCACTCGCTTAAAAGAGAACTATGA
ACTGCTGTGGAACATCGGCTCAAACGATGGCTATCTGCACTTAGTGGGTATCATGCAAAAGTTCGTTGACCAAGCGATCT
CTGCCAATACTAACTACGATCCAAGCAATTTCGATTCTGGCAAAGTACCGATGAAAAAACTGCTCCAAGATCTGCTGACA
GCGTATAAGTTTGGTGTGAAAACCTTGTACTACCATAACACTCGTGATGGCGCGAAAGACGAGCAGACCGATGCTACGGT
GAAAGTGCAGGAAGACGATTGTGCCAGCGGCGCTTGTAAGATCTAA

Upstream 100 bases:

>100_bases
CTATATGTAGGGCGAGCTCGATCACAAAAGCCTCGATTCAATTTACAAGATTTAGACAAAAACACGGCTTTTATCAGTTT
TGTTAGGGAAATGAAGCAGA

Downstream 100 bases:

>100_bases
GCGCCTGCTTCTAAAGCACAACACGAGAAAATAGTTTAACTCTTTGATTTGCATGCCTCTTGCTTAGGCAAGAGGCCAAA
CGGATGAAGGCAACAATGGC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 761; Mature: 761

Protein sequence:

>761_residues
MNQQLTVTKRDGRKENIDLDKIHRVITWAAEGLQNVSVSQVELRAHIQFYDGITTSDIHETIIKSAADLISEETPDYQYL
AARLAVFHLRKKAYGQYEPPTLLAHVTKMVEKGKYDKHLLEDYTREELELMDQFIDHRRDLDFSYAAVKQLEGKYFVQNR
VTGQIYESAQFLYILVAACLFAKYPKETRLDYIKRFYDATSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATVFYPLWHGEAESLLVLK
NNRGVEENRVRHMDYGVQLNKLMYKRLVEGGNITLFSPSDVPGLYDAFFQDQDEFERLYVKYENDPSIKKKTVKALEIFT
LLMQERASTGRIYIQNVDHCNTHSPFDAAVAPVRQSNLCLEIALPTSPLANVEDESGEIALCTLSAFNLGAIESLDDFEE
LSDLVVRALDALLDYQDYPLPAARRSSMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHRTFEAMQYHLLRASMKLAK
EQGRCPLFHETNYAKGLMPIDTYKKDIDLVCAEPLHYDWDKLRHEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRG
YVSVKASKDGILKQVVPEFTRLKENYELLWNIGSNDGYLHLVGIMQKFVDQAISANTNYDPSNFDSGKVPMKKLLQDLLT
AYKFGVKTLYYHNTRDGAKDEQTDATVKVQEDDCASGACKI

Sequences:

>Translated_761_residues
MNQQLTVTKRDGRKENIDLDKIHRVITWAAEGLQNVSVSQVELRAHIQFYDGITTSDIHETIIKSAADLISEETPDYQYL
AARLAVFHLRKKAYGQYEPPTLLAHVTKMVEKGKYDKHLLEDYTREELELMDQFIDHRRDLDFSYAAVKQLEGKYFVQNR
VTGQIYESAQFLYILVAACLFAKYPKETRLDYIKRFYDATSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATVFYPLWHGEAESLLVLK
NNRGVEENRVRHMDYGVQLNKLMYKRLVEGGNITLFSPSDVPGLYDAFFQDQDEFERLYVKYENDPSIKKKTVKALEIFT
LLMQERASTGRIYIQNVDHCNTHSPFDAAVAPVRQSNLCLEIALPTSPLANVEDESGEIALCTLSAFNLGAIESLDDFEE
LSDLVVRALDALLDYQDYPLPAARRSSMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHRTFEAMQYHLLRASMKLAK
EQGRCPLFHETNYAKGLMPIDTYKKDIDLVCAEPLHYDWDKLRHEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRG
YVSVKASKDGILKQVVPEFTRLKENYELLWNIGSNDGYLHLVGIMQKFVDQAISANTNYDPSNFDSGKVPMKKLLQDLLT
AYKFGVKTLYYHNTRDGAKDEQTDATVKVQEDDCASGACKI
>Mature_761_residues
MNQQLTVTKRDGRKENIDLDKIHRVITWAAEGLQNVSVSQVELRAHIQFYDGITTSDIHETIIKSAADLISEETPDYQYL
AARLAVFHLRKKAYGQYEPPTLLAHVTKMVEKGKYDKHLLEDYTREELELMDQFIDHRRDLDFSYAAVKQLEGKYFVQNR
VTGQIYESAQFLYILVAACLFAKYPKETRLDYIKRFYDATSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATVFYPLWHGEAESLLVLK
NNRGVEENRVRHMDYGVQLNKLMYKRLVEGGNITLFSPSDVPGLYDAFFQDQDEFERLYVKYENDPSIKKKTVKALEIFT
LLMQERASTGRIYIQNVDHCNTHSPFDAAVAPVRQSNLCLEIALPTSPLANVEDESGEIALCTLSAFNLGAIESLDDFEE
LSDLVVRALDALLDYQDYPLPAARRSSMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHRTFEAMQYHLLRASMKLAK
EQGRCPLFHETNYAKGLMPIDTYKKDIDLVCAEPLHYDWDKLRHEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRG
YVSVKASKDGILKQVVPEFTRLKENYELLWNIGSNDGYLHLVGIMQKFVDQAISANTNYDPSNFDSGKVPMKKLLQDLLT
AYKFGVKTLYYHNTRDGAKDEQTDATVKVQEDDCASGACKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=792, Percent_Identity=27.3989898989899, Blast_Score=241, Evalue=2e-63,
Organism=Escherichia coli, GI2367133, Length=761, Percent_Identity=80.1576872536137, Blast_Score=1305, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=657, Percent_Identity=24.3531202435312, Blast_Score=157, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17555242, Length=776, Percent_Identity=27.0618556701031, Blast_Score=237, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6322123, Length=784, Percent_Identity=27.9336734693878, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6320914, Length=784, Percent_Identity=27.5510204081633, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17352481, Length=782, Percent_Identity=27.8772378516624, Blast_Score=262, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 85851; Mature: 85851

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQLTVTKRDGRKENIDLDKIHRVITWAAEGLQNVSVSQVELRAHIQFYDGITTSDIHE
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEHHEEEEEHCCCCCHHHHHH
TIIKSAADLISEETPDYQYLAARLAVFHLRKKAYGQYEPPTLLAHVTKMVEKGKYDKHLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHH
EDYTREELELMDQFIDHRRDLDFSYAAVKQLEGKYFVQNRVTGQIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEHHCCCHHHHHHHHHHHHHHHHHH
FAKYPKETRLDYIKRFYDATSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCCHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCHHHHHHH
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCC
AATVFYPLWHGEAESLLVLKNNRGVEENRVRHMDYGVQLNKLMYKRLVEGGNITLFSPSD
CEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFQDQDEFERLYVKYENDPSIKKKTVKALEIFTLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDAAVAPVRQSNLCLEIALPTSPLANVEDESGEIALCTLSAFNLGAIESLDDFEE
CCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
LSDLVVRALDALLDYQDYPLPAARRSSMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCH
HRTFEAMQYHLLRASMKLAKEQGRCPLFHETNYAKGLMPIDTYKKDIDLVCAEPLHYDWD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHCCCCEEECCCCCCCHH
KLRHEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRGYVSVKASKDGILKQVVPEFT
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
RLKENYELLWNIGSNDGYLHLVGIMQKFVDQAISANTNYDPSNFDSGKVPMKKLLQDLLT
HHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKFGVKTLYYHNTRDGAKDEQTDATVKVQEDDCASGACKI
HHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNQQLTVTKRDGRKENIDLDKIHRVITWAAEGLQNVSVSQVELRAHIQFYDGITTSDIHE
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEHHEEEEEHCCCCCHHHHHH
TIIKSAADLISEETPDYQYLAARLAVFHLRKKAYGQYEPPTLLAHVTKMVEKGKYDKHLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHH
EDYTREELELMDQFIDHRRDLDFSYAAVKQLEGKYFVQNRVTGQIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEHHCCCHHHHHHHHHHHHHHHHHH
FAKYPKETRLDYIKRFYDATSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
HHHCCCHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCHHHHHHH
ASSIVRYVSQRAGIGINAGRIRALGSEIRGGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCC
AATVFYPLWHGEAESLLVLKNNRGVEENRVRHMDYGVQLNKLMYKRLVEGGNITLFSPSD
CEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFQDQDEFERLYVKYENDPSIKKKTVKALEIFTLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDAAVAPVRQSNLCLEIALPTSPLANVEDESGEIALCTLSAFNLGAIESLDDFEE
CCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
LSDLVVRALDALLDYQDYPLPAARRSSMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCH
HRTFEAMQYHLLRASMKLAKEQGRCPLFHETNYAKGLMPIDTYKKDIDLVCAEPLHYDWD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHCCCCEEECCCCCCCHH
KLRHEIMEHGLRNSTLTALMPSETSSQISNATNGIEPPRGYVSVKASKDGILKQVVPEFT
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHH
RLKENYELLWNIGSNDGYLHLVGIMQKFVDQAISANTNYDPSNFDSGKVPMKKLLQDLLT
HHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AYKFGVKTLYYHNTRDGAKDEQTDATVKVQEDDCASGACKI
HHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]