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The map label for this gene is trpE

Identifier: 227081346

GI number: 227081346

Start: 1204766

End: 1206337

Strand: Reverse

Name: trpE

Synonym: VCM66_1129

Alternate gene names: 227081346

Gene position: 1206337-1204766 (Counterclockwise)

Preceding gene: 227081347

Following gene: 227081345

Centisome position: 41.71

GC content: 51.34

Gene sequence:

>1572_bases
ATGAATAAGGCCATCAACATAAAGAAAACCGCGCCGCTTGAGGTGCTACACAGTGAATTGCCTTATACGCAAGATCCTAC
GGCTTTATTTCATGCTCTTTGCGCTGGGCGCAGCGACTGCCTACTACTGGAATCTGCAGAAATCGACTCCAAGCAAAATT
TGAAGAGCCTTCTGTTAGTGGATGCTGCCGTGCGCATTGTGTGTGAAGGCCATCAAGTGACCTATCATGCGCTCAGTGCA
AACGGCCAAGCGCTGCTCAACATCATTCATAGCAACCTCACTGATCGGATTCCTTGCAAAGTAGAGAAAGCGAAACTTAC
CCTCACCTTCTCTACACCCTGTGATACGTTGGATGAAGACTCACGATTGCGCGAAGCGTCTTCTTTCGATGCGCTACGTT
TAGTGCAGCACAGTTTTGATCTCACTGACCACGGTAAATTTGCGCTGTTTTTAGGTGGCTTATTTGCCTACGATTTAGTG
GCTAACTTTGAACCGCTCGGTGAAGCACCTGCCGACAATCAATGCCCTGATTACGTATTTTATGTAGCAGAAACTCTGAT
GGTGATTGATCATCAGCGTGAAACTTGCCAACTGCAAGCCACCCAATTCCAACCGGGCGATGCGCTGCACAGCCAACTCA
AAAGCCGGATGCGTGAAATTCGTGCGCAAGTGAATCAAAAATTGCCTTTGCCGAGTGCGCAATCTTTATCTGATGTGGAA
GTGACCACCAATATTAGCGATGCAGCATTTTGCGATATCGTGCGTGACCTCAAGCAGTACGTGGTCAAAGGCGATGTGTT
CCAAGTTGTGCCTTCGCGCCGTTTTCGCTTACCTTGCCCTTCACCACTCGCAGCTTATCAACGGCTGAAACAGAGTAACC
CAAGCCCTTACATGTTCTACATGCAAGATGAACGCTTTACCCTGTTTGGCGCATCCCCCGAAAGCGCACTCAAGTATGAA
ATGCACACCAACCAAGTGGAAATCTACCCGATTGCAGGGACTCGCCGCCGCGGTAAGCGCGCCGATGGCAGCATCGATTT
TGACCTCGATAGCCGCATTGAGCTTGAACTGCGCACCGATAAAAAAGAGAACGCCGAACACATGATGCTGGTTGACTTAG
CACGCAACGATGTCGCGCGCATTAGCCAAGCCGGTACTCGCCATGTCGCTGACTTGCTGCAAGTAGATCGCTACAGCCAT
GTGATGCACTTGGTGTCGCGCGTGGTGGGTCAGTTACGTGAAGATCTGGATGCGCTGCATGCTTATCAAGCTTGCATGAA
CATGGGCACGCTGACTGGCGCACCGAAAATTCGCGCGATGCAGTTAATCCGCGATGTGGAACAAGCGCGTCGCGGCAGCT
ACGGCGGCGCGGTGGGTTATCTCACGGGTGAAGGCGATTTGGATACCTGTATCGTGATCCGTTCTGCTTATGTGGAAAAC
GGCATCGCCCAAGTCCAAGCTGGCGCGGGTGTCGTTTACGACTCCGACCCACAAGCCGAAGCCGATGAAACGCGCGGCAA
GGCGCAAGCGGTAATCTCCGCTATTTTATATGCTCATCAAGGGAAGGAATGA

Upstream 100 bases:

>100_bases
GAGCGGGCTTTTTATTGGTTTTTTCCACCTATCACTGCTGTTTCGCTACTTTCTCAACGCGGTGATCAGTGCACAATCGA
ATCATGGTTAAGGAGGTCTT

Downstream 100 bases:

>100_bases
TCATGGCCAATATTCTGTTTATCGATAACTTTGACTCCTTCACTTATAACTTGGTGGATCAGTTTCGCTCACTCGGTCAC
GTGGTCACGATTTATCGCAA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNKAINIKKTAPLEVLHSELPYTQDPTALFHALCAGRSDCLLLESAEIDSKQNLKSLLLVDAAVRIVCEGHQVTYHALSA
NGQALLNIIHSNLTDRIPCKVEKAKLTLTFSTPCDTLDEDSRLREASSFDALRLVQHSFDLTDHGKFALFLGGLFAYDLV
ANFEPLGEAPADNQCPDYVFYVAETLMVIDHQRETCQLQATQFQPGDALHSQLKSRMREIRAQVNQKLPLPSAQSLSDVE
VTTNISDAAFCDIVRDLKQYVVKGDVFQVVPSRRFRLPCPSPLAAYQRLKQSNPSPYMFYMQDERFTLFGASPESALKYE
MHTNQVEIYPIAGTRRRGKRADGSIDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARISQAGTRHVADLLQVDRYSH
VMHLVSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEQARRGSYGGAVGYLTGEGDLDTCIVIRSAYVEN
GIAQVQAGAGVVYDSDPQAEADETRGKAQAVISAILYAHQGKE

Sequences:

>Translated_523_residues
MNKAINIKKTAPLEVLHSELPYTQDPTALFHALCAGRSDCLLLESAEIDSKQNLKSLLLVDAAVRIVCEGHQVTYHALSA
NGQALLNIIHSNLTDRIPCKVEKAKLTLTFSTPCDTLDEDSRLREASSFDALRLVQHSFDLTDHGKFALFLGGLFAYDLV
ANFEPLGEAPADNQCPDYVFYVAETLMVIDHQRETCQLQATQFQPGDALHSQLKSRMREIRAQVNQKLPLPSAQSLSDVE
VTTNISDAAFCDIVRDLKQYVVKGDVFQVVPSRRFRLPCPSPLAAYQRLKQSNPSPYMFYMQDERFTLFGASPESALKYE
MHTNQVEIYPIAGTRRRGKRADGSIDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARISQAGTRHVADLLQVDRYSH
VMHLVSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEQARRGSYGGAVGYLTGEGDLDTCIVIRSAYVEN
GIAQVQAGAGVVYDSDPQAEADETRGKAQAVISAILYAHQGKE
>Mature_523_residues
MNKAINIKKTAPLEVLHSELPYTQDPTALFHALCAGRSDCLLLESAEIDSKQNLKSLLLVDAAVRIVCEGHQVTYHALSA
NGQALLNIIHSNLTDRIPCKVEKAKLTLTFSTPCDTLDEDSRLREASSFDALRLVQHSFDLTDHGKFALFLGGLFAYDLV
ANFEPLGEAPADNQCPDYVFYVAETLMVIDHQRETCQLQATQFQPGDALHSQLKSRMREIRAQVNQKLPLPSAQSLSDVE
VTTNISDAAFCDIVRDLKQYVVKGDVFQVVPSRRFRLPCPSPLAAYQRLKQSNPSPYMFYMQDERFTLFGASPESALKYE
MHTNQVEIYPIAGTRRRGKRADGSIDFDLDSRIELELRTDKKENAEHMMLVDLARNDVARISQAGTRHVADLLQVDRYSH
VMHLVSRVVGQLREDLDALHAYQACMNMGTLTGAPKIRAMQLIRDVEQARRGSYGGAVGYLTGEGDLDTCIVIRSAYVEN
GIAQVQAGAGVVYDSDPQAEADETRGKAQAVISAILYAHQGKE

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787518, Length=511, Percent_Identity=60.665362035225, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1788114, Length=441, Percent_Identity=30.8390022675737, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786809, Length=230, Percent_Identity=30, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082077, Length=268, Percent_Identity=27.9850746268657, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=379, Percent_Identity=35.6200527704486, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6324361, Length=383, Percent_Identity=25.065274151436, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_VIBCH (Q9KST2)

Other databases:

- EMBL:   AE003852
- PIR:   A82233
- RefSeq:   NP_230819.1
- ProteinModelPortal:   Q9KST2
- SMR:   Q9KST2
- GeneID:   2614607
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1174
- TIGR:   VC_1174
- HOGENOM:   HBG507440
- OMA:   HKELAEH
- ProtClustDB:   PRK13564
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR00565

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 58119; Mature: 58119

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAINIKKTAPLEVLHSELPYTQDPTALFHALCAGRSDCLLLESAEIDSKQNLKSLLLV
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
DAAVRIVCEGHQVTYHALSANGQALLNIIHSNLTDRIPCKVEKAKLTLTFSTPCDTLDED
HHHHHHEECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCH
SRLREASSFDALRLVQHSFDLTDHGKFALFLGGLFAYDLVANFEPLGEAPADNQCPDYVF
HHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
YVAETLMVIDHQRETCQLQATQFQPGDALHSQLKSRMREIRAQVNQKLPLPSAQSLSDVE
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
VTTNISDAAFCDIVRDLKQYVVKGDVFQVVPSRRFRLPCPSPLAAYQRLKQSNPSPYMFY
EECCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
MQDERFTLFGASPESALKYEMHTNQVEIYPIAGTRRRGKRADGSIDFDLDSRIELELRTD
EECCEEEEEECCCCHHEEEEEECCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEEEECC
KKENAEHMMLVDLARNDVARISQAGTRHVADLLQVDRYSHVMHLVSRVVGQLREDLDALH
CCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYQACMNMGTLTGAPKIRAMQLIRDVEQARRGSYGGAVGYLTGEGDLDTCIVIRSAYVEN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHC
GIAQVQAGAGVVYDSDPQAEADETRGKAQAVISAILYAHQGKE
CHHHEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKAINIKKTAPLEVLHSELPYTQDPTALFHALCAGRSDCLLLESAEIDSKQNLKSLLLV
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
DAAVRIVCEGHQVTYHALSANGQALLNIIHSNLTDRIPCKVEKAKLTLTFSTPCDTLDED
HHHHHHEECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCH
SRLREASSFDALRLVQHSFDLTDHGKFALFLGGLFAYDLVANFEPLGEAPADNQCPDYVF
HHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
YVAETLMVIDHQRETCQLQATQFQPGDALHSQLKSRMREIRAQVNQKLPLPSAQSLSDVE
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
VTTNISDAAFCDIVRDLKQYVVKGDVFQVVPSRRFRLPCPSPLAAYQRLKQSNPSPYMFY
EECCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
MQDERFTLFGASPESALKYEMHTNQVEIYPIAGTRRRGKRADGSIDFDLDSRIELELRTD
EECCEEEEEECCCCHHEEEEEECCEEEEEECCCCCCCCCCCCCCEEECCCCEEEEEEECC
KKENAEHMMLVDLARNDVARISQAGTRHVADLLQVDRYSHVMHLVSRVVGQLREDLDALH
CCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYQACMNMGTLTGAPKIRAMQLIRDVEQARRGSYGGAVGYLTGEGDLDTCIVIRSAYVEN
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHC
GIAQVQAGAGVVYDSDPQAEADETRGKAQAVISAILYAHQGKE
CHHHEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301