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The map label for this gene is trpG [H]

Identifier: 227081345

GI number: 227081345

Start: 1204158

End: 1204769

Strand: Reverse

Name: trpG [H]

Synonym: VCM66_1128

Alternate gene names: 227081345

Gene position: 1204769-1204158 (Counterclockwise)

Preceding gene: 227081346

Following gene: 227081344

Centisome position: 41.65

GC content: 52.78

Gene sequence:

>612_bases
ATGATCATGGCCAATATTCTGTTTATCGATAACTTTGACTCCTTCACTTATAACTTGGTGGATCAGTTTCGCTCACTCGG
TCACGTGGTCACGATTTATCGCAATAACCTTTCTGCGGATGCTATCGAGCAAGCGCTGCTGCAACTGGATAATCCTGTGG
TCGTGTTATCCCCCGGGCCAGGTGCGCCGTCTGAAACGGGCTGCATGCCAGAGCTGCTGCAACGCCTGAAAGGCAAAGTG
CCAATGATTGGTATCTGCTTAGGGCATCAAGCCATCGTGGAAGCCTACGGCGGTGTGGTTGCTGGCGCGGGTGAAATCAT
CCACGGTAAAGTATCGATGATGGAGCACCAAAACCACGCGATTTATCGCGGTCTCCCTTCACCGTTGGCGATCGCACGTT
ACCACTCACTGGTCGCCACGCAAGTTCCATCAGCACTGACCGTGACGGCAGAAGTGAACGGACTCGTGATGTCGGTGGTT
AATGAGGCTGACAAAGTCTGCGGCTTTCAATTTCACCCAGAATCGATCATGACCACCCACGGCGCTACCTTGCTGGCCAA
TGCCATTGATTGGGCATTGTCTTCAACGCCAGCGCAGACTCAATTCGCGTAA

Upstream 100 bases:

>100_bases
GTGTCGTTTACGACTCCGACCCACAAGCCGAAGCCGATGAAACGCGCGGCAAGGCGCAAGCGGTAATCTCCGCTATTTTA
TATGCTCATCAAGGGAAGGA

Downstream 100 bases:

>100_bases
CCGCACAGGGAGAGAATGTTATGCAAACCATTATTAATAAACTCTATGAACAGCAAGGGCTCACGCAAGCCGAGAGCCAA
CAGCTTTTCGACCAAATTAT

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIMANILFIDNFDSFTYNLVDQFRSLGHVVTIYRNNLSADAIEQALLQLDNPVVVLSPGPGAPSETGCMPELLQRLKGKV
PMIGICLGHQAIVEAYGGVVAGAGEIIHGKVSMMEHQNHAIYRGLPSPLAIARYHSLVATQVPSALTVTAEVNGLVMSVV
NEADKVCGFQFHPESIMTTHGATLLANAIDWALSSTPAQTQFA

Sequences:

>Translated_203_residues
MIMANILFIDNFDSFTYNLVDQFRSLGHVVTIYRNNLSADAIEQALLQLDNPVVVLSPGPGAPSETGCMPELLQRLKGKV
PMIGICLGHQAIVEAYGGVVAGAGEIIHGKVSMMEHQNHAIYRGLPSPLAIARYHSLVATQVPSALTVTAEVNGLVMSVV
NEADKVCGFQFHPESIMTTHGATLLANAIDWALSSTPAQTQFA
>Mature_203_residues
MIMANILFIDNFDSFTYNLVDQFRSLGHVVTIYRNNLSADAIEQALLQLDNPVVVLSPGPGAPSETGCMPELLQRLKGKV
PMIGICLGHQAIVEAYGGVVAGAGEIIHGKVSMMEHQNHAIYRGLPSPLAIARYHSLVATQVPSALTVTAEVNGLVMSVV
NEADKVCGFQFHPESIMTTHGATLLANAIDWALSSTPAQTQFA

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=199, Percent_Identity=61.3065326633166, Blast_Score=264, Evalue=3e-72,
Organism=Escherichia coli, GI1789760, Length=186, Percent_Identity=41.3978494623656, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322638, Length=194, Percent_Identity=40.7216494845361, Blast_Score=152, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324361, Length=209, Percent_Identity=33.4928229665072, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21710; Mature: 21710

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMANILFIDNFDSFTYNLVDQFRSLGHVVTIYRNNLSADAIEQALLQLDNPVVVLSPGP
CEEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
GAPSETGCMPELLQRLKGKVPMIGICLGHQAIVEAYGGVVAGAGEIIHGKVSMMEHQNHA
CCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHCCEEECCCCEEEHHHHHHHCCCCC
IYRGLPSPLAIARYHSLVATQVPSALTVTAEVNGLVMSVVNEADKVCGFQFHPESIMTTH
EECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEECCCHHHHHC
GATLLANAIDWALSSTPAQTQFA
CHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIMANILFIDNFDSFTYNLVDQFRSLGHVVTIYRNNLSADAIEQALLQLDNPVVVLSPGP
CEEEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
GAPSETGCMPELLQRLKGKVPMIGICLGHQAIVEAYGGVVAGAGEIIHGKVSMMEHQNHA
CCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHCCEEECCCCEEEHHHHHHHCCCCC
IYRGLPSPLAIARYHSLVATQVPSALTVTAEVNGLVMSVVNEADKVCGFQFHPESIMTTH
EECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEECCCHHHHHC
GATLLANAIDWALSSTPAQTQFA
CHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]