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The map label for this gene is trpB [H]

Identifier: 227081342

GI number: 227081342

Start: 1200493

End: 1201683

Strand: Reverse

Name: trpB [H]

Synonym: VCM66_1125

Alternate gene names: 227081342

Gene position: 1201683-1200493 (Counterclockwise)

Preceding gene: 227081343

Following gene: 227081341

Centisome position: 41.54

GC content: 50.46

Gene sequence:

>1191_bases
ATGGCTAAATTAAACGCCTATTTTGGCGAATTCGGTGGGCAGTTTGTGCCACAAATTTTGGTTCCGGCACTCGATCAACT
GGAACAAGCTTTTATTGATGCCCAGCAGGATGATGCTTTTCGCGCTGAATTTATGAGCCTACTGCAAGAGTACGCGGGTC
GCCCCACTGCGCTGACACTCACGCAAAACATCACCAAAGGCACCAAAACCAAACTCTATTTGAAGCGTGAAGATCTACTG
CACGGCGGCGCGCACAAAACCAACCAAGTTTTGGGCCAAGCCCTGCTCGCCAAACGTATGGGCAAACATGAAATTATCGC
CGAGACAGGCGCAGGTCAACACGGTGTCGCAACCGCATTAGCCTGTGCACTGCTTGGCCTAAAATGCCGCGTGTACATGG
GTGCAAAAGACGTTGAGCGCCAAAGCCCAAACGTGTTCCGTATGCGCTTAATGGGTGCCACCGTGATCCCTGTTCATTCA
GGTTCAGCAACGCTAAAAGATGCCTGTAACGAAGCGCTGCGTGACTGGTCAGCCAGCTATGAAACTGCGCATTATCTGCT
CGGTACTGCGGCAGGCCCTCACCCATTCCCAACCATAGTGCGTGAATTCCAACGCATTATTGGTGAAGAAACCAAAAACC
AAATCCTTGCTCGTGAAGGTCGTTTGCCCGATGCGGTGATCGCTTGTGTCGGTGGCGGCTCAAACGCGATTGGTATGTTT
GCCGATTTCATCGAAGAAGAATCCGTACGTTTGATCGGTATTGAGCCAGCAGGTAAAGGCATTCATACCCATCAACATGG
TGCACCACTCAAACACGGTAAAACTGGTATCTTCTTCGGCATGAAAGCACCGTTGATGCAAGATGAACATGGTCAAGTGG
AAGAGTCTTACTCTGTCTCTGCGGGGCTGGATTTCCCATCGGTGGGTCCACAACATGCTTACCTCAACGCCATTGGTCGT
GCGGAATATGAAAGCATTACCGATGATGAAGCACTCGATGCGTTCCAAGCGTTGGCACGTAACGAAGGCATCATTCCAGC
GCTGGAGTCTTCACACGCTCTCGCGCATGCAATCAAAATGGCGTATGCCGAACCAGACAAAGAGCAGTTGCTCGTGGTTA
ACCTTTCGGGCCGAGGTGATAAGGATATTTTCACCGTACACAAATTACTTGAAGACAAAGGAGCGTTGTAA

Upstream 100 bases:

>100_bases
CCGGGTAAAAAAGACGCCAACCAGTTACAACAGGTTTTCCAACAGTTACGGAATTATTAGATTATCAGCATGGCAACATG
CATAAGAAAGGAATGTAAGC

Downstream 100 bases:

>100_bases
TGAACCGTTATCAAGCCCTATTTCAACGCCTGAGCGCCGCTCAGCAAGGCGCTTTCGTTCCTTTCGTGACCATTGGTGAC
CCAAACCCCGAGCAGTCACT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKLNAYFGEFGGQFVPQILVPALDQLEQAFIDAQQDDAFRAEFMSLLQEYAGRPTALTLTQNITKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKHEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMRLMGATVIPVHS
GSATLKDACNEALRDWSASYETAHYLLGTAAGPHPFPTIVREFQRIIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
ADFIEEESVRLIGIEPAGKGIHTHQHGAPLKHGKTGIFFGMKAPLMQDEHGQVEESYSVSAGLDFPSVGPQHAYLNAIGR
AEYESITDDEALDAFQALARNEGIIPALESSHALAHAIKMAYAEPDKEQLLVVNLSGRGDKDIFTVHKLLEDKGAL

Sequences:

>Translated_396_residues
MAKLNAYFGEFGGQFVPQILVPALDQLEQAFIDAQQDDAFRAEFMSLLQEYAGRPTALTLTQNITKGTKTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKHEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMRLMGATVIPVHS
GSATLKDACNEALRDWSASYETAHYLLGTAAGPHPFPTIVREFQRIIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
ADFIEEESVRLIGIEPAGKGIHTHQHGAPLKHGKTGIFFGMKAPLMQDEHGQVEESYSVSAGLDFPSVGPQHAYLNAIGR
AEYESITDDEALDAFQALARNEGIIPALESSHALAHAIKMAYAEPDKEQLLVVNLSGRGDKDIFTVHKLLEDKGAL
>Mature_395_residues
AKLNAYFGEFGGQFVPQILVPALDQLEQAFIDAQQDDAFRAEFMSLLQEYAGRPTALTLTQNITKGTKTKLYLKREDLLH
GGAHKTNQVLGQALLAKRMGKHEIIAETGAGQHGVATALACALLGLKCRVYMGAKDVERQSPNVFRMRLMGATVIPVHSG
SATLKDACNEALRDWSASYETAHYLLGTAAGPHPFPTIVREFQRIIGEETKNQILAREGRLPDAVIACVGGGSNAIGMFA
DFIEEESVRLIGIEPAGKGIHTHQHGAPLKHGKTGIFFGMKAPLMQDEHGQVEESYSVSAGLDFPSVGPQHAYLNAIGRA
EYESITDDEALDAFQALARNEGIIPALESSHALAHAIKMAYAEPDKEQLLVVNLSGRGDKDIFTVHKLLEDKGAL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=393, Percent_Identity=81.4249363867684, Blast_Score=669, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=390, Percent_Identity=52.5641025641026, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 42864; Mature: 42733

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLNAYFGEFGGQFVPQILVPALDQLEQAFIDAQQDDAFRAEFMSLLQEYAGRPTALTL
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
TQNITKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKHEIIAETGAGQHGVATAL
ECCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMRLMGATVIPVHSGSATLKDACNEALRDWSASY
HHHHHHHHEEEEECCHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCH
ETAHYLLGTAAGPHPFPTIVREFQRIIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
HHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIEEESVRLIGIEPAGKGIHTHQHGAPLKHGKTGIFFGMKAPLMQDEHGQVEESYSVS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAYLNAIGRAEYESITDDEALDAFQALARNEGIIPALESSHALAHAIKM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AYAEPDKEQLLVVNLSGRGDKDIFTVHKLLEDKGAL
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKLNAYFGEFGGQFVPQILVPALDQLEQAFIDAQQDDAFRAEFMSLLQEYAGRPTALTL
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
TQNITKGTKTKLYLKREDLLHGGAHKTNQVLGQALLAKRMGKHEIIAETGAGQHGVATAL
ECCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
ACALLGLKCRVYMGAKDVERQSPNVFRMRLMGATVIPVHSGSATLKDACNEALRDWSASY
HHHHHHHHEEEEECCHHHHHCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCH
ETAHYLLGTAAGPHPFPTIVREFQRIIGEETKNQILAREGRLPDAVIACVGGGSNAIGMF
HHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHH
ADFIEEESVRLIGIEPAGKGIHTHQHGAPLKHGKTGIFFGMKAPLMQDEHGQVEESYSVS
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCC
AGLDFPSVGPQHAYLNAIGRAEYESITDDEALDAFQALARNEGIIPALESSHALAHAIKM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AYAEPDKEQLLVVNLSGRGDKDIFTVHKLLEDKGAL
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]