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The map label for this gene is trpC/F

Identifier: 227081343

GI number: 227081343

Start: 1201724

End: 1203133

Strand: Reverse

Name: trpC/F

Synonym: VCM66_1126

Alternate gene names: 227081343

Gene position: 1203133-1201724 (Counterclockwise)

Preceding gene: 227081344

Following gene: 227081342

Centisome position: 41.59

GC content: 49.86

Gene sequence:

>1410_bases
ATGTCCGAGCAGTTGTCTGAGCATATTTCCGTACAAACCGCGCAAATGGCGCAAGTGCTGGTCAAAATCGTCAACGATAA
ATACCAATGGATTGAAGAGCGCAAAGCCAAGCAACCTCTTGCTAGCTTTCATCCATTACTGACTCGCTCAGAGCGCGATT
TCTATCAAGCCTTGAGCGGCGATAACACCGTATTCATTCTCGAATGCAAAAAAGCCTCACCTTCAAAAGGATTGATCCGC
GAAGAGTTTGATCTCGATTACATCGCCTCTGTGTATGGCGAGTACGCCAGTGCCATTTCGGTACTGACTGACGAGAAATA
TTTCCAAGGTCGTTTTGAGTTTTTGCCGCAAGTGCGCGCTCAAGTGGCTCAGCCTGTCTTGTGCAAAGATTTTATGGTCG
ACCCGTATCAGGTTTATCTGGCGCGTCATTATCAAGCGGATGCGATTTTGCTGATGCTGTCTGTGCTCAATGATGATCAA
TATCGTGAACTGGCTGCTGTGGCGCACGACCTTGGTATGGGCGTACTCACCGAAGTCAGCAACGAACAAGAATTGAAACG
TGCAGTTGATCTGCAAGCGAAAGTCATCGGCATCAATAACCGCAATCTGCGTGATTTGACCACCGACCTAAATCGCACCA
AGCAGTTGGCGCCGCTCATTCCACAAGGCACCATCATTATTTCCGAATCCGGTATTTATACCCATCAGCAGGTGCGTGAT
TTGGCTGAATTTGCCAATGGTTTCTTGATTGGTAGCTCGCTGATGGCGCAAAGCAATCTTGAACTGGCGGTGCGAAAAAT
TGTGCTAGGTGAACATAAGGTGTGCGGTTTGACTCATCCTGATGATGCGGTGAAAGCCTACCAAGCCGGTTCCGTTTTTG
GTGGATTGATTTTTGTTGAGAAGTCCAAACGCTTTGTCGATGTAGAACAAGCTCGCCTGACCATGAGCGGCGCGCCGCTA
CAGTATGTCGGCGTGTTTCAAAATCACCCAGCAGCACAAGTCGCCGATATTGCAACCAAACTTGGCTTATTTGCGGTGCA
ACTGCACGGTGAGGAAGACTCTGCGTATGTAAGCGAACTGCGCGCTTCCCTTCCAGAATCCATTGAAATTTGGAAAGCGT
ATGGCGTGAGTGATGCTTTACCTGAGCTACTACCAGAGCACATCGATAGGCATCTGCTCGATGCCAAAGTCGGCGAACAG
AGTGGCGGCACAGGACGCAGTTTTGATTGGCGCTTGCTGCCAAAACATAGCGACCTGATGCTCGCCGGCGGCTTGAGCGC
CGAGAATGTCGCCCAAGCGGCGCAACTTGGCTGCCGCGGACTCGATTTCAACTCTGGCGTGGAAAGCGCTCCGGGTAAAA
AAGACGCCAACCAGTTACAACAGGTTTTCCAACAGTTACGGAATTATTAG

Upstream 100 bases:

>100_bases
AAGAAGATCTGAAAGCCAACACCCAACAAGCGATCGCCGTGATGAAATCGGGCCAAGCCTATGGATTGGTTCAGCAGTTA
GCACAACGAGGTTAAGCAGA

Downstream 100 bases:

>100_bases
ATTATCAGCATGGCAACATGCATAAGAAAGGAATGTAAGCATGGCTAAATTAAACGCCTATTTTGGCGAATTCGGTGGGC
AGTTTGTGCCACAAATTTTG

Product: bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Products: NA

Alternate protein names: Indole-3-glycerol phosphate synthase; IGPS; N-(5'-phospho-ribosyl)anthranilate isomerase; PRAI

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSEQLSEHISVQTAQMAQVLVKIVNDKYQWIEERKAKQPLASFHPLLTRSERDFYQALSGDNTVFILECKKASPSKGLIR
EEFDLDYIASVYGEYASAISVLTDEKYFQGRFEFLPQVRAQVAQPVLCKDFMVDPYQVYLARHYQADAILLMLSVLNDDQ
YRELAAVAHDLGMGVLTEVSNEQELKRAVDLQAKVIGINNRNLRDLTTDLNRTKQLAPLIPQGTIIISESGIYTHQQVRD
LAEFANGFLIGSSLMAQSNLELAVRKIVLGEHKVCGLTHPDDAVKAYQAGSVFGGLIFVEKSKRFVDVEQARLTMSGAPL
QYVGVFQNHPAAQVADIATKLGLFAVQLHGEEDSAYVSELRASLPESIEIWKAYGVSDALPELLPEHIDRHLLDAKVGEQ
SGGTGRSFDWRLLPKHSDLMLAGGLSAENVAQAAQLGCRGLDFNSGVESAPGKKDANQLQQVFQQLRNY

Sequences:

>Translated_469_residues
MSEQLSEHISVQTAQMAQVLVKIVNDKYQWIEERKAKQPLASFHPLLTRSERDFYQALSGDNTVFILECKKASPSKGLIR
EEFDLDYIASVYGEYASAISVLTDEKYFQGRFEFLPQVRAQVAQPVLCKDFMVDPYQVYLARHYQADAILLMLSVLNDDQ
YRELAAVAHDLGMGVLTEVSNEQELKRAVDLQAKVIGINNRNLRDLTTDLNRTKQLAPLIPQGTIIISESGIYTHQQVRD
LAEFANGFLIGSSLMAQSNLELAVRKIVLGEHKVCGLTHPDDAVKAYQAGSVFGGLIFVEKSKRFVDVEQARLTMSGAPL
QYVGVFQNHPAAQVADIATKLGLFAVQLHGEEDSAYVSELRASLPESIEIWKAYGVSDALPELLPEHIDRHLLDAKVGEQ
SGGTGRSFDWRLLPKHSDLMLAGGLSAENVAQAAQLGCRGLDFNSGVESAPGKKDANQLQQVFQQLRNY
>Mature_468_residues
SEQLSEHISVQTAQMAQVLVKIVNDKYQWIEERKAKQPLASFHPLLTRSERDFYQALSGDNTVFILECKKASPSKGLIRE
EFDLDYIASVYGEYASAISVLTDEKYFQGRFEFLPQVRAQVAQPVLCKDFMVDPYQVYLARHYQADAILLMLSVLNDDQY
RELAAVAHDLGMGVLTEVSNEQELKRAVDLQAKVIGINNRNLRDLTTDLNRTKQLAPLIPQGTIIISESGIYTHQQVRDL
AEFANGFLIGSSLMAQSNLELAVRKIVLGEHKVCGLTHPDDAVKAYQAGSVFGGLIFVEKSKRFVDVEQARLTMSGAPLQ
YVGVFQNHPAAQVADIATKLGLFAVQLHGEEDSAYVSELRASLPESIEIWKAYGVSDALPELLPEHIDRHLLDAKVGEQS
GGTGRSFDWRLLPKHSDLMLAGGLSAENVAQAAQLGCRGLDFNSGVESAPGKKDANQLQQVFQQLRNY

Specific function: Bifunctional enzyme that catalyzes two sequential steps of tryptophan biosynthetic pathway. The first reaction is catalyzed by the isomerase, coded by the trpF domain; the second reaction is catalyzed by the synthase, coded by the trpC domain

COG id: COG0134

COG function: function code E; Indole-3-glycerol phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=456, Percent_Identity=60.9649122807018, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322638, Length=236, Percent_Identity=37.2881355932203, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320210, Length=221, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPC_VIBCH (Q9KST5)

Other databases:

- EMBL:   AE003852
- PIR:   F82232
- RefSeq:   NP_230816.1
- ProteinModelPortal:   Q9KST5
- SMR:   Q9KST5
- GeneID:   2614604
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1171
- TIGR:   VC_1171
- HOGENOM:   HBG296890
- OMA:   YILECKK
- ProtClustDB:   PRK09427
- BRENDA:   4.1.1.48
- BRENDA:   5.3.1.24
- HAMAP:   MF_00134_B
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR013798
- InterPro:   IPR001468
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00218 IGPS; PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =4.1.1.48; =5.3.1.24

Molecular weight: Translated: 52039; Mature: 51908

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00614 IGPS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLSEHISVQTAQMAQVLVKIVNDKYQWIEERKAKQPLASFHPLLTRSERDFYQALSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCC
DNTVFILECKKASPSKGLIREEFDLDYIASVYGEYASAISVLTDEKYFQGRFEFLPQVRA
CCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QVAQPVLCKDFMVDPYQVYLARHYQADAILLMLSVLNDDQYRELAAVAHDLGMGVLTEVS
HHCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCC
NEQELKRAVDLQAKVIGINNRNLRDLTTDLNRTKQLAPLIPQGTIIISESGIYTHQQVRD
CHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHH
LAEFANGFLIGSSLMAQSNLELAVRKIVLGEHKVCGLTHPDDAVKAYQAGSVFGGLIFVE
HHHHHCCEEECHHHHHHCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHEEEEEEE
KSKRFVDVEQARLTMSGAPLQYVGVFQNHPAAQVADIATKLGLFAVQLHGEEDSAYVSEL
CCCCEEEHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCCHHHHHHH
RASLPESIEIWKAYGVSDALPELLPEHIDRHLLDAKVGEQSGGTGRSFDWRLLPKHSDLM
HHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEE
LAGGLSAENVAQAAQLGCRGLDFNSGVESAPGKKDANQLQQVFQQLRNY
EECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEQLSEHISVQTAQMAQVLVKIVNDKYQWIEERKAKQPLASFHPLLTRSERDFYQALSG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCC
DNTVFILECKKASPSKGLIREEFDLDYIASVYGEYASAISVLTDEKYFQGRFEFLPQVRA
CCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QVAQPVLCKDFMVDPYQVYLARHYQADAILLMLSVLNDDQYRELAAVAHDLGMGVLTEVS
HHCCCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHCC
NEQELKRAVDLQAKVIGINNRNLRDLTTDLNRTKQLAPLIPQGTIIISESGIYTHQQVRD
CHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHH
LAEFANGFLIGSSLMAQSNLELAVRKIVLGEHKVCGLTHPDDAVKAYQAGSVFGGLIFVE
HHHHHCCEEECHHHHHHCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHEEEEEEE
KSKRFVDVEQARLTMSGAPLQYVGVFQNHPAAQVADIATKLGLFAVQLHGEEDSAYVSEL
CCCCEEEHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHCEEEEEEECCCCHHHHHHH
RASLPESIEIWKAYGVSDALPELLPEHIDRHLLDAKVGEQSGGTGRSFDWRLLPKHSDLM
HHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEE
LAGGLSAENVAQAAQLGCRGLDFNSGVESAPGKKDANQLQQVFQQLRNY
EECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301