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The map label for this gene is trpA

Identifier: 227081341

GI number: 227081341

Start: 1199687

End: 1200493

Strand: Reverse

Name: trpA

Synonym: VCM66_1124

Alternate gene names: 227081341

Gene position: 1200493-1199687 (Counterclockwise)

Preceding gene: 227081342

Following gene: 227081340

Centisome position: 41.5

GC content: 51.55

Gene sequence:

>807_bases
ATGAACCGTTATCAAGCCCTATTTCAACGCCTGAGCGCCGCTCAGCAAGGCGCTTTCGTTCCTTTCGTGACCATTGGTGA
CCCAAACCCCGAGCAGTCACTCGCCATCATGCAAACCCTGATTGATGCAGGAGCGGATGCGCTTGAGCTTGGCATGCCGT
TTTCAGATCCTCTGGCCGATGGTCCGACGATTCAAGGCGCGAACTTACGTGCATTAGCGGCGAAAACCACGCCAGATATC
TGTTTTGAGCTGATCGCGCAGATCCGTGCGCGTAACCCTGAGACTCCGATTGGCTTACTGATGTATGCCAACTTGGTTTA
CGCACGCGGCATCGACGATTTTTATCAACGCTGCCAAAAAGCTGGCGTGGATTCGGTACTGATTGCCGATGTACCCACCA
ACGAAAGCCAGCCTTTTGTGGCGGCCGCAGAGAAATTTGGCATTCAACCAATCTTTATTGCGCCACCAACGGCCAGTGAT
GAAACCTTACGAGCAGTTGCTCAACTCGGTAAAGGCTACACTTATTTGCTTTCGCGCGCTGGGGTGACTGGGGCTGAAAC
CAAAGCCAACATGCCTGTGCATGCCCTGCTTGAACGTCTGCAACAATTTGATGCGCCACCAGCACTGCTAGGTTTTGGTA
TTTCAGAGCCCGCTCAAGTTAAACAAGCGATTGAAGCTGGCGCAGCGGGAGCCATTTCCGGATCGGCGGTGGTAAAAATC
ATCGAAACGCATTTGGATAATCCAGCTAAGCAGCTGACTGAGCTGGCAAACTTCACTCAAGCCATGAAAAAAGCAACAAA
AATTTAA

Upstream 100 bases:

>100_bases
AACCAGACAAAGAGCAGTTGCTCGTGGTTAACCTTTCGGGCCGAGGTGATAAGGATATTTTCACCGTACACAAATTACTT
GAAGACAAAGGAGCGTTGTA

Downstream 100 bases:

>100_bases
CTTACATTACCTTAAATCAGCGGCGCATCCTTTGGTGTGCCGCATCATTTCATCAACAACTCCACTTAAACAAAAGACAT
TTCAATCAAAAATCAATTCA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNRYQALFQRLSAAQQGAFVPFVTIGDPNPEQSLAIMQTLIDAGADALELGMPFSDPLADGPTIQGANLRALAAKTTPDI
CFELIAQIRARNPETPIGLLMYANLVYARGIDDFYQRCQKAGVDSVLIADVPTNESQPFVAAAEKFGIQPIFIAPPTASD
ETLRAVAQLGKGYTYLLSRAGVTGAETKANMPVHALLERLQQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKI
IETHLDNPAKQLTELANFTQAMKKATKI

Sequences:

>Translated_268_residues
MNRYQALFQRLSAAQQGAFVPFVTIGDPNPEQSLAIMQTLIDAGADALELGMPFSDPLADGPTIQGANLRALAAKTTPDI
CFELIAQIRARNPETPIGLLMYANLVYARGIDDFYQRCQKAGVDSVLIADVPTNESQPFVAAAEKFGIQPIFIAPPTASD
ETLRAVAQLGKGYTYLLSRAGVTGAETKANMPVHALLERLQQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKI
IETHLDNPAKQLTELANFTQAMKKATKI
>Mature_268_residues
MNRYQALFQRLSAAQQGAFVPFVTIGDPNPEQSLAIMQTLIDAGADALELGMPFSDPLADGPTIQGANLRALAAKTTPDI
CFELIAQIRARNPETPIGLLMYANLVYARGIDDFYQRCQKAGVDSVLIADVPTNESQPFVAAAEKFGIQPIFIAPPTASD
ETLRAVAQLGKGYTYLLSRAGVTGAETKANMPVHALLERLQQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKI
IETHLDNPAKQLTELANFTQAMKKATKI

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=62.9213483146067, Blast_Score=328, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6321412, Length=235, Percent_Identity=32.3404255319149, Blast_Score=129, Evalue=6e-31,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_VIBC3 (A5F207)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F207
- SMR:   A5F207
- STRING:   A5F207
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0791
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   VCHO345073:VC0395_A0791-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28494; Mature: 28494

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYQALFQRLSAAQQGAFVPFVTIGDPNPEQSLAIMQTLIDAGADALELGMPFSDPLAD
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
GPTIQGANLRALAAKTTPDICFELIAQIRARNPETPIGLLMYANLVYARGIDDFYQRCQK
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AGVDSVLIADVPTNESQPFVAAAEKFGIQPIFIAPPTASDETLRAVAQLGKGYTYLLSRA
CCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGAETKANMPVHALLERLQQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKI
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
IETHLDNPAKQLTELANFTQAMKKATKI
HHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRYQALFQRLSAAQQGAFVPFVTIGDPNPEQSLAIMQTLIDAGADALELGMPFSDPLAD
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
GPTIQGANLRALAAKTTPDICFELIAQIRARNPETPIGLLMYANLVYARGIDDFYQRCQK
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
AGVDSVLIADVPTNESQPFVAAAEKFGIQPIFIAPPTASDETLRAVAQLGKGYTYLLSRA
CCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGAETKANMPVHALLERLQQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKI
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
IETHLDNPAKQLTELANFTQAMKKATKI
HHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA