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The map label for this gene is ackA-1

Identifier: 227081271

GI number: 227081271

Start: 1125185

End: 1126381

Strand: Reverse

Name: ackA-1

Synonym: VCM66_1054

Alternate gene names: 227081271

Gene position: 1126381-1125185 (Counterclockwise)

Preceding gene: 227081273

Following gene: 227081270

Centisome position: 38.94

GC content: 50.54

Gene sequence:

>1197_bases
ATGTCTAAGCTGGTTTTAGTTTTAAACTGCGGTAGTTCTTCACTTAAGTTCGCAGTTGTTGACGCTCAAAGCGGTGAACA
ACATCTTTCAGGTCTCGCTGAATGTTTACACCTTCCTGAAGCTCGTATCAAGTGGAAATTGGACGGCAAGCACGAAGCTC
AACTGGGCGAAGGCGCTGCACACGATGAAGCTCTGTCTTTCATCGTTGAAACTATTCTTGCTTCTAAACCAGAACTGGCA
GCTCAACTGAAAGCGATCGGTCACCGCGTGGTACACGGTGGTGAGCAGTTCACTCAATCTGCACTGATTGACGACGCTGT
ACTGAAAGGCATCGAAGATTGTGCAGCACTCGCTCCACTGCACAACCCAGCACACATCATCGGTATCAAAGCGGCGCAAA
AAGCGTTCCCAGAGCTGAAAAATGTTGCGGTATTCGACACTGCGTTCCACCAAACAATGCCAGAAGAAGCGTACCTGTAC
GCTCTGCCATACAACCTGTACAAAGAGCACGGTATTCGTCGCTACGGCATGCACGGTACGTCTCACCTGTTCATCGCACG
TGAAGCGGCTGAGCGTCTGGGCAAACCTGCAAACGAACTGAACATCATCAACTGCCACCTAGGCAACGGTGCTTCTGTGT
GTGCGATCAAAAACGGCCAATCGGTTGACACATCTATGGGTCTGACTCCACTGGAAGGTCTGGTGATGGGTACTCGTTGT
GGTGATATCGATCCTGCGATCATTTTCCACCTGCACGACACGCTGGGTTACTCAGTTGAGAAAATCAACACCATGCTGAC
CAAAGAATCGGGTCTGTTGGGTCTGACTGAAGTGACTTCTGACTGCCGTTTCGTTGAAGACAACTACGGCCAAAAAGAAG
AAGCAACTCGCGCAATGGACGTATTCTGTCACCGCCTAGCGAAATACGTTGCTGGCTACACAGCAAGCCTAGACGGCCGT
CTGGACGCGATCACCTTCACTGGTGGTATCGGTGAAAACTCTGCGCCAATCCGTGAAATGGTTCTGAACCGTCTAGCGAT
TTTCGGTATCACCGTTGACAGCGCAGCAAACCTGAAAGCCCGTTTCGGCGGTGAAGGTGTGATCACCACTGCAGACAGCC
GTATCCCAGCGATGGTTATCGCGACTAACGAAGAGTTAGTGATTGCAGAAGATACAGCTCGTCTGACGAACATCTAA

Upstream 100 bases:

>100_bases
TTGACGTTTTATTGACCGAGATTAGGGCGCATTCATCGAGGCGTCATCTACTCTTGGTACTGATTTTTTTATCCCAACCG
ATTATTACAGGTAGTCACTC

Downstream 100 bases:

>100_bases
TACGACTTACACTGGCTAACTCAGGTTAGCCAGTGTCATTTAGGGGCTCAGCGTAAGCCTGTCCTGCGTTTGAGCTTTGT
CACTTTCAATCTAAGCACCT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKLVLVLNCGSSSLKFAVVDAQSGEQHLSGLAECLHLPEARIKWKLDGKHEAQLGEGAAHDEALSFIVETILASKPELA
AQLKAIGHRVVHGGEQFTQSALIDDAVLKGIEDCAALAPLHNPAHIIGIKAAQKAFPELKNVAVFDTAFHQTMPEEAYLY
ALPYNLYKEHGIRRYGMHGTSHLFIAREAAERLGKPANELNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
GDIDPAIIFHLHDTLGYSVEKINTMLTKESGLLGLTEVTSDCRFVEDNYGQKEEATRAMDVFCHRLAKYVAGYTASLDGR
LDAITFTGGIGENSAPIREMVLNRLAIFGITVDSAANLKARFGGEGVITTADSRIPAMVIATNEELVIAEDTARLTNI

Sequences:

>Translated_398_residues
MSKLVLVLNCGSSSLKFAVVDAQSGEQHLSGLAECLHLPEARIKWKLDGKHEAQLGEGAAHDEALSFIVETILASKPELA
AQLKAIGHRVVHGGEQFTQSALIDDAVLKGIEDCAALAPLHNPAHIIGIKAAQKAFPELKNVAVFDTAFHQTMPEEAYLY
ALPYNLYKEHGIRRYGMHGTSHLFIAREAAERLGKPANELNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
GDIDPAIIFHLHDTLGYSVEKINTMLTKESGLLGLTEVTSDCRFVEDNYGQKEEATRAMDVFCHRLAKYVAGYTASLDGR
LDAITFTGGIGENSAPIREMVLNRLAIFGITVDSAANLKARFGGEGVITTADSRIPAMVIATNEELVIAEDTARLTNI
>Mature_397_residues
SKLVLVLNCGSSSLKFAVVDAQSGEQHLSGLAECLHLPEARIKWKLDGKHEAQLGEGAAHDEALSFIVETILASKPELAA
QLKAIGHRVVHGGEQFTQSALIDDAVLKGIEDCAALAPLHNPAHIIGIKAAQKAFPELKNVAVFDTAFHQTMPEEAYLYA
LPYNLYKEHGIRRYGMHGTSHLFIAREAAERLGKPANELNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRCG
DIDPAIIFHLHDTLGYSVEKINTMLTKESGLLGLTEVTSDCRFVEDNYGQKEEATRAMDVFCHRLAKYVAGYTASLDGRL
DAITFTGGIGENSAPIREMVLNRLAIFGITVDSAANLKARFGGEGVITTADSRIPAMVIATNEELVIAEDTARLTNI

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=73.1155778894472, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI145693184, Length=400, Percent_Identity=45, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA1_VIBCH (Q9KT07)

Other databases:

- EMBL:   AE003852
- PIR:   A82243
- RefSeq:   NP_230743.1
- ProteinModelPortal:   Q9KT07
- SMR:   Q9KT07
- GeneID:   2614368
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1098
- TIGR:   VC_1098
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- ProtClustDB:   PRK00180
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42843; Mature: 42712

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVLVLNCGSSSLKFAVVDAQSGEQHLSGLAECLHLPEARIKWKLDGKHEAQLGEGAA
CCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
HDEALSFIVETILASKPELAAQLKAIGHRVVHGGEQFTQSALIDDAVLKGIEDCAALAPL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHIIGIKAAQKAFPELKNVAVFDTAFHQTMPEEAYLYALPYNLYKEHGIRRYGMHGT
CCCCEEEEEHHHHHHHHHHHCEEEEEHHHHHCCCCCEEEEEECHHHHHHCCCEEECCCCC
SHLFIAREAAERLGKPANELNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
CEEEEEHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDIDPAIIFHLHDTLGYSVEKINTMLTKESGLLGLTEVTSDCRFVEDNYGQKEEATRAMD
CCCCCEEEEEEHHHHCCHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTASLDGRLDAITFTGGIGENSAPIREMVLNRLAIFGITVDSAANLKA
HHHHHHHHHHCCCCEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECCCCCCEE
RFGGEGVITTADSRIPAMVIATNEELVIAEDTARLTNI
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure 
SKLVLVLNCGSSSLKFAVVDAQSGEQHLSGLAECLHLPEARIKWKLDGKHEAQLGEGAA
CCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
HDEALSFIVETILASKPELAAQLKAIGHRVVHGGEQFTQSALIDDAVLKGIEDCAALAPL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HNPAHIIGIKAAQKAFPELKNVAVFDTAFHQTMPEEAYLYALPYNLYKEHGIRRYGMHGT
CCCCEEEEEHHHHHHHHHHHCEEEEEHHHHHCCCCCEEEEEECHHHHHHCCCEEECCCCC
SHLFIAREAAERLGKPANELNIINCHLGNGASVCAIKNGQSVDTSMGLTPLEGLVMGTRC
CEEEEEHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEECCC
GDIDPAIIFHLHDTLGYSVEKINTMLTKESGLLGLTEVTSDCRFVEDNYGQKEEATRAMD
CCCCCEEEEEEHHHHCCHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHH
VFCHRLAKYVAGYTASLDGRLDAITFTGGIGENSAPIREMVLNRLAIFGITVDSAANLKA
HHHHHHHHHHCCCCEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECCCCCCEE
RFGGEGVITTADSRIPAMVIATNEELVIAEDTARLTNI
EECCCEEEEECCCCCCEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301