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The map label for this gene is rnfA [H]

Identifier: 227081191

GI number: 227081191

Start: 1043762

End: 1044403

Strand: Reverse

Name: rnfA [H]

Synonym: VCM66_0973

Alternate gene names: 227081191

Gene position: 1044403-1043762 (Counterclockwise)

Preceding gene: 227081193

Following gene: 227081190

Centisome position: 36.11

GC content: 47.35

Gene sequence:

>642_bases
TTGTTATTGCTTTGGCAAAGCAGGATAATGCCGGGATCGGAAGCAAACATTTATATAACTATGACCGAATATCTTTTGTT
GTTAATCGGCACCGTGCTGGTCAATAACTTTGTACTGGTGAAGTTTTTGGGCTTATGTCCTTTTATGGGCGTATCAAAAA
AACTAGAGACCGCCATTGGCATGGGGTTGGCGACGACATTCGTCCTCACCTTAGCTTCGGTGTGCGCTTATCTGGTGGAA
AGTTACGTGTTACGTCCGCTCGGCATTGAGTATCTGCGCACCATGAGCTTTATTTTGGTGATCGCTGTCGTAGTACAGTT
CACCGAAATGGTGGTGCACAAAACCAGTCCGACACTCTATCGCCTGCTGGGCATTTTCCTGCCACTCATCACCACCAACT
GTGCGGTATTAGGGGTTGCGCTGCTCAACATCAACGAAAATCACAACTTTATTCAATCGATCATTTATGGTTTTGGCGCT
GCTGTTGGCTTCTCGCTGGTGCTCATCTTGTTCGCTTCAATGCGTGAGCGAATCCATGTAGCCGATGTCCCCGCTCCCTT
TAAGGGCGCATCCATTGCGATGATCACCGCAGGTTTAATGTCTTTGGCCTTTATGGGCTTTACCGGATTGGTGAAACTGT
AA

Upstream 100 bases:

>100_bases
TCCCGTCAAGATCTCTTATCAATCCCTGCCATTCGCATCCCATTTGCCACTTATTGCGTATAATTTATCTCCATCTACCT
AAAACCTTATCTTTCCTTGG

Downstream 100 bases:

>100_bases
TGAGTACCATAGTCATCGCTGTTATTGCGCTGGCGGCATTGGCCGCCGTGTTTGGAGCCATACTCGGCTTTGCTTCGATT
CGCTTTAAGGTAGAAGCCGA

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLLLWQSRIMPGSEANIYITMTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVCAYLVE
SYVLRPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGA
AVGFSLVLILFASMRERIHVADVPAPFKGASIAMITAGLMSLAFMGFTGLVKL

Sequences:

>Translated_213_residues
MLLLWQSRIMPGSEANIYITMTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVCAYLVE
SYVLRPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGA
AVGFSLVLILFASMRERIHVADVPAPFKGASIAMITAGLMSLAFMGFTGLVKL
>Mature_213_residues
MLLLWQSRIMPGSEANIYITMTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVCAYLVE
SYVLRPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGA
AVGFSLVLILFASMRERIHVADVPAPFKGASIAMITAGLMSLAFMGFTGLVKL

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=80.8290155440415, Blast_Score=320, Evalue=5e-89,
Organism=Escherichia coli, GI1787919, Length=158, Percent_Identity=34.1772151898734, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 23264; Mature: 23264

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLWQSRIMPGSEANIYITMTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLETAIG
CEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MGLATTFVLTLASVCAYLVESYVLRPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGAAVGFSLVLILFASMRERIHV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
ADVPAPFKGASIAMITAGLMSLAFMGFTGLVKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLLWQSRIMPGSEANIYITMTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLETAIG
CEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MGLATTFVLTLASVCAYLVESYVLRPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGAAVGFSLVLILFASMRERIHV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
ADVPAPFKGASIAMITAGLMSLAFMGFTGLVKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA