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The map label for this gene is rnt [H]

Identifier: 227081180

GI number: 227081180

Start: 1034856

End: 1035587

Strand: Reverse

Name: rnt [H]

Synonym: VCM66_0962

Alternate gene names: 227081180

Gene position: 1035587-1034856 (Counterclockwise)

Preceding gene: 227081181

Following gene: 227081179

Centisome position: 35.8

GC content: 46.58

Gene sequence:

>732_bases
TTGCTCTACCGCAGAATCTGCAATAATGCCAGCCTTATCACACAATTTAGAACCCGCATGACTGATAAAAATGACGCGCT
AACCTTGAAAAAACGCTTTCGTGGCTATTTTCCTGTGGTTATCGATGTAGAGACCGCAGGATTCAATGCGCAAACCGATG
CGCTTTTAGAAATTTGTGCTGTCACCTTGAGTATGGATGAAAACGGCGATTTACACCCAGCAAGTACTATCCATTTTCAT
GTCGAACCCTTTGAAGGCGCGAATCTCGAAAAAGCCGCACTAGAATTTACTGGCATTTACGACCCATTTAGCCCACTACG
GGGGGCGGTGAGTGAAGATCATGCATTAAAAGAGATTTACAAACTGGTTCGCAAAGAGCAAAAAGCCGCAGATTGCAGCC
GCGCCATTATTGTGGCGCATAACGCGCATTTCGATCACAGTTTCGTGATGGCAGCGAGCGAACGTTGTAAATTAAAACGT
GTTCCTTTCCATCCCTTTGCCACGTTTGACACGGCAACGCTAAGTGGTCTTGCTTTCGGGCAGACCGTGCTGGCTAAAGC
CTGTAAAACTGCTGGGATGGAATTTGACAATCGCGAGGCACATTCCGCCTTGTACGATACCCAAAAAACTGCAGAGCTGT
TCTGCACCATTGTCAACCAGTGGAAAGCTTTAGGTGGCTGGCCATTGGTCAATGATGATGAGGACAATAACAACGATGCC
GATTTAGATTGA

Upstream 100 bases:

>100_bases
ACAACTTGCTTGCCGCAAAACAGCCTTGAATGAATCCCTTTCAACCTTATCGCTTCTCTTTATTGAACAATTAATTGCCG
CGATTTAGATAAGCTAGGTT

Downstream 100 bases:

>100_bases
AGCTCGTTCTACCCATGGACCGCTTTAACCAATCAGGCCCATCCCTACCCTTCTTGCTTCTTCAAGTGGGCTGGGGATAC
ACAACATCCCGAGAAAAAAT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLYRRICNNASLITQFRTRMTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICAVTLSMDENGDLHPASTIHFH
VEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIYKLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKR
VPFHPFATFDTATLSGLAFGQTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDA
DLD

Sequences:

>Translated_243_residues
MLYRRICNNASLITQFRTRMTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICAVTLSMDENGDLHPASTIHFH
VEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIYKLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKR
VPFHPFATFDTATLSGLAFGQTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDA
DLD
>Mature_243_residues
MLYRRICNNASLITQFRTRMTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICAVTLSMDENGDLHPASTIHFH
VEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIYKLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKR
VPFHPFATFDTATLSGLAFGQTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDA
DLD

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=215, Percent_Identity=68.8372093023256, Blast_Score=303, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 27151; Mature: 27151

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRRICNNASLITQFRTRMTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICA
CCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
VTLSMDENGDLHPASTIHFHVEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIY
HHEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHEECCCCCCHHHHHCCCCCHHHHHHHH
KLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKRVPFHPFATFDTATLSGLAFG
HHHHHHHHHHCCCCEEEEEECCCCCCHHEEECCCCCCEECCCCCCCCCCCHHHHHHHHHH
QTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DLD
CCC
>Mature Secondary Structure
MLYRRICNNASLITQFRTRMTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICA
CCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
VTLSMDENGDLHPASTIHFHVEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIY
HHEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHEECCCCCCHHHHHCCCCCHHHHHHHH
KLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKRVPFHPFATFDTATLSGLAFG
HHHHHHHHHHCCCCEEEEEECCCCCCHHEEECCCCCCEECCCCCCCCCCCHHHHHHHHHH
QTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
DLD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]