Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 227081136

GI number: 227081136

Start: 983610

End: 985034

Strand: Reverse

Name: miaB

Synonym: VCM66_0918

Alternate gene names: 227081136

Gene position: 985034-983610 (Counterclockwise)

Preceding gene: 227081138

Following gene: 227081135

Centisome position: 34.05

GC content: 50.04

Gene sequence:

>1425_bases
ATGAGTAAGAAACTGCTAATTAAAACTTGGGGCTGCCAGATGAACGAGTACGATTCATCGAAAATGGCTGACCTGCTTAA
TGCTGCAAACGGCTATGAGCTGACAGAAATACCCGAGGAAGCTGACGTTTTACTCCTAAACACCTGTTCGATTCGTGAAA
AGGCGCAGGAAAAAGTCTTCCACCAACTCGGTCGTTGGAAAACCCTGAAAGACAAGAAGCCAGGTGTCGTGATCGGTGTC
GGTGGCTGTGTTGCTACTCAAGAAGGTGACTCGATTCGCGATCGTGCGCCTTATGTGGATGTCATCTTCGGCCCACAGAC
GCTGCATCGTCTGCCAGAAATGATTAAACAATCGCAAACCAGCGACGCGCCCGTGATGGATATCTCTTTCCCTGAGATTG
AAAAATTCGACCGCCTGCCTGAGCCACGTGCGGAAGGCCCAACGGCGTTCGTTTCTATCATGGAAGGCTGCTCAAAATAC
TGTACTTACTGCGTAGTGCCTTACACACGCGGTGAAGAAGTCAGCCGTCCTATGGACGATGTCCTGTTCGAGATCGCTCA
GCTTGCCGAGCAAGGGGTGCGTGAAGTTAACCTGCTGGGTCAAAACGTCAACGCTTACCGTGGTGCCACTCACGATGGCG
GCATTTGCTCTTTCGCTGAATTACTGCGTTTGGTCGCCACCATTGACGGCATCGATCGTATCCGCTTTACCACTAGCCAC
CCACTCGAATTTACCGATGACATTATTGCAGTGTACGAAGACACGCCTGAGCTGGTGAGCTTCCTGCACTTGCCAGTACA
AAGTGGGAGCGATCGCATTCTGACTATGATGAAGCGTCCACATACGGCGATTGAGTACAAATCCATCATCCGTAAGCTAC
GTAAAGCACGCCCAGATATTCAAATCAGCTCTGATTTTATCGTTGGCTTCCCAGGGGAAACCGACAAAGATTTCCAAGAC
ACCATGAAGCTGATCCGCGATGTCGATTTCGACATGAGCTTTAGCTTTATTTTCTCACCTCGTCCAGGCACACCCGCCGC
TGACTATCCTTGCGATCTGTCGGAAGAAGTGAAGAAAGAACGTCTGTATGAACTGCAACAGCAGATCAACAGCCAAGCTA
TGCGTTATTCACGCCTGATGCTGGGTACTGAACAGCGTATTTTGGTTGAAGGCCCCTCGAAAAAAGATCTGATGGAGCTG
CGTGGCCGTACTGAAAATAACCGTGTAGTGAACTTCGAAGGCTCACCGGATCTGATCGGTCAATTTGTTGATGTGAAGAT
CGTTGACGTTTTCCCGAACTCACTGCGCGGTGAACTGCTGCGTACCGAACAAGAGATGAATCTGCGTATCGCGACCTCTC
CCTCTGAAATGAAAGCGAAGACTCGCCGCGAGGATGAGCTTGGGGTTGCTACATTTACTCCTTAA

Upstream 100 bases:

>100_bases
TTTTTAAAGCAGTACAATACGCCGCTTACCGCCGAGAGGGCGGCTAGAATGTGAGCTACAGCTCAAATGATTGAAGAACT
ATTGAACGAGCGAAAGTGAG

Downstream 100 bases:

>100_bases
TCTCGTATACACTGTCAATAAGCGTCCGGTCACAATCGGACGTTTATTGACATACTGAGAGGCAAATTTGAGCAATAAAA
TCATAACCTTAGAAATCAAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKPGVVIGV
GGCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQTSDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPMDDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSH
PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGETDKDFQD
TMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKERLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMEL
RGRTENNRVVNFEGSPDLIGQFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP

Sequences:

>Translated_474_residues
MSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKPGVVIGV
GGCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQTSDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPMDDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSH
PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGETDKDFQD
TMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKERLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMEL
RGRTENNRVVNFEGSPDLIGQFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP
>Mature_473_residues
SKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKPGVVIGVG
GCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQTSDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYC
TYCVVPYTRGEEVSRPMDDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSHP
LEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQISSDFIVGFPGETDKDFQDT
MKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKERLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELR
GRTENNRVVNFEGSPDLIGQFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=36.5344467640919, Blast_Score=289, Evalue=5e-78,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=36.5344467640919, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI93277076, Length=448, Percent_Identity=29.9107142857143, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=77.4261603375527, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=385, Percent_Identity=30.3896103896104, Blast_Score=169, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=33.4056399132321, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=255, Percent_Identity=32.9411764705882, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=30.8533916849015, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_VIBC3 (A5F2X6)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2X6
- SMR:   A5F2X6
- STRING:   A5F2X6
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0484
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- BioCyc:   VCHO345073:VC0395_A0484-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53614; Mature: 53483

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVF
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKKPGVVIGVGGCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQT
HHHHCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCC
SDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCC
PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETDKDFQDTMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKE
EECCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
RLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELRGRTENNRVVNFEGSPDLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHH
QFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP
HHHCEEEEEECCCHHHHHHHHCHHHCCEEEECCCHHHHHHHCCCCCCCCEECCC
>Mature Secondary Structure 
SKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVF
CCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKKPGVVIGVGGCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQT
HHHHCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCC
SDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCC
PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETDKDFQDTMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKE
EECCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHH
RLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELRGRTENNRVVNFEGSPDLIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHH
QFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP
HHHCEEEEEECCCHHHHHHHHCHHHCCEEEECCCHHHHHHHCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA