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The map label for this gene is lipB [H]

Identifier: 227081118

GI number: 227081118

Start: 967196

End: 967855

Strand: Reverse

Name: lipB [H]

Synonym: VCM66_0900

Alternate gene names: 227081118

Gene position: 967855-967196 (Counterclockwise)

Preceding gene: 227081119

Following gene: 227081117

Centisome position: 33.46

GC content: 46.06

Gene sequence:

>660_bases
ATGACAATAATGGAAAACCAGCTGTTGGTGAGACGCCTTGGCAGACAGGACTACACTCCCGTTTGGCAGGCAATGCATCA
ATTCACTGATCAACGTGACTCCACAACGCGTGATGAAGTGTGGCTTGTCGAACACAACCCCGTGTTTACTCAAGGACAAG
CCGGCAAAGCTGAGCACCTCCTCAATACTGGAGATATTCCTGTTGTACAAAGTGATCGGGGTGGGCAAGTCACCTACCAC
GGACCGGGGCAATTAGTCGCCTATTTTCTGATTGACCTACGCCGTAAAAAGCTCGGCGTCCGTGAATTAGTGACACACAT
TGAAAATTTGGTTATTCACACCTTAAAACATTATCAAATTGAGTCAGCCGCACGACCCGATGCCCCCGGAGTTTACGTAC
AAAATAGAAAAATCTGCTCTTTAGGTTTGCGTATCCGTAAGGGATGCTCTTTCCACGGACTCGCACTCAATATTCAGATG
GATCTTGCTCCATTTTTACGTATTAACCCTTGTGGGTATGCTGGAATGGAGATGATTCAATTGAGTGACCTGCATCCGGT
CAGCATGGAACAGGTTGAAAAAGTATTAATCCAAGAACTCGTTACTCTACTGGACTACGAGCAAGTCGAATTCAGCACAG
AAGCATACAATCATGAGTAA

Upstream 100 bases:

>100_bases
ACAAAACCATCATTTTATCAGGGTCAAAAAGTTCCCCCTGATGTGTAGTTCATTACAGGTAAGCAGTTTATAATCGAAAA
ACTTTATATATCTCTGGCGG

Downstream 100 bases:

>100_bases
ACCAATTCAGATGGAACGCGGCGTTAAATATCGTGACGCCGACAAAATGGCTTTGATTCCCATCAAGAACATGCCGACAG
AGCAGAAAGAAGTTCTGCGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTIMENQLLVRRLGRQDYTPVWQAMHQFTDQRDSTTRDEVWLVEHNPVFTQGQAGKAEHLLNTGDIPVVQSDRGGQVTYH
GPGQLVAYFLIDLRRKKLGVRELVTHIENLVIHTLKHYQIESAARPDAPGVYVQNRKICSLGLRIRKGCSFHGLALNIQM
DLAPFLRINPCGYAGMEMIQLSDLHPVSMEQVEKVLIQELVTLLDYEQVEFSTEAYNHE

Sequences:

>Translated_219_residues
MTIMENQLLVRRLGRQDYTPVWQAMHQFTDQRDSTTRDEVWLVEHNPVFTQGQAGKAEHLLNTGDIPVVQSDRGGQVTYH
GPGQLVAYFLIDLRRKKLGVRELVTHIENLVIHTLKHYQIESAARPDAPGVYVQNRKICSLGLRIRKGCSFHGLALNIQM
DLAPFLRINPCGYAGMEMIQLSDLHPVSMEQVEKVLIQELVTLLDYEQVEFSTEAYNHE
>Mature_218_residues
TIMENQLLVRRLGRQDYTPVWQAMHQFTDQRDSTTRDEVWLVEHNPVFTQGQAGKAEHLLNTGDIPVVQSDRGGQVTYHG
PGQLVAYFLIDLRRKKLGVRELVTHIENLVIHTLKHYQIESAARPDAPGVYVQNRKICSLGLRIRKGCSFHGLALNIQMD
LAPFLRINPCGYAGMEMIQLSDLHPVSMEQVEKVLIQELVTLLDYEQVEFSTEAYNHE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=144, Percent_Identity=36.8055555555556, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87081767, Length=210, Percent_Identity=62.8571428571429, Blast_Score=282, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6323268, Length=157, Percent_Identity=32.484076433121, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355483, Length=218, Percent_Identity=28.8990825688073, Blast_Score=90, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25048; Mature: 24917

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIMENQLLVRRLGRQDYTPVWQAMHQFTDQRDSTTRDEVWLVEHNPVFTQGQAGKAEHL
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCHHHH
LNTGDIPVVQSDRGGQVTYHGPGQLVAYFLIDLRRKKLGVRELVTHIENLVIHTLKHYQI
CCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
ESAARPDAPGVYVQNRKICSLGLRIRKGCSFHGLALNIQMDLAPFLRINPCGYAGMEMIQ
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCHHEEE
LSDLHPVSMEQVEKVLIQELVTLLDYEQVEFSTEAYNHE
ECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIMENQLLVRRLGRQDYTPVWQAMHQFTDQRDSTTRDEVWLVEHNPVFTQGQAGKAEHL
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCHHHH
LNTGDIPVVQSDRGGQVTYHGPGQLVAYFLIDLRRKKLGVRELVTHIENLVIHTLKHYQI
CCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
ESAARPDAPGVYVQNRKICSLGLRIRKGCSFHGLALNIQMDLAPFLRINPCGYAGMEMIQ
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCCCCCHHEEE
LSDLHPVSMEQVEKVLIQELVTLLDYEQVEFSTEAYNHE
ECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA