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The map label for this gene is lipA [H]

Identifier: 227081117

GI number: 227081117

Start: 966238

End: 967203

Strand: Reverse

Name: lipA [H]

Synonym: VCM66_0899

Alternate gene names: 227081117

Gene position: 967203-966238 (Counterclockwise)

Preceding gene: 227081118

Following gene: 227081116

Centisome position: 33.44

GC content: 48.14

Gene sequence:

>966_bases
ATGAGTAAACCAATTCAGATGGAACGCGGCGTTAAATATCGTGACGCCGACAAAATGGCTTTGATTCCCATCAAGAACAT
GCCGACAGAGCAGAAAGAAGTTCTGCGCAAACCAGAATGGATGAAGATAAAGCTACCTGCGGATAGCCAGCGCATTCAAG
ACATCAAGGCCGCCATGCGTAAAAATAATCTTCACTCAGTCTGTGAAGAAGCCTCCTGCCCTAACTTGGCTGAGTGTTTT
AACCATGGTACGGCGACCTTTATGATTTTAGGTGCTATCTGTACCCGTCGCTGCCCATTCTGTGACGTTGCCCATGGCCG
CCCCAATGCACCAGAAGCGGAAGAGCCGAAAAAGCTTGCGCAAACCATTCACGATATGAAGCTCAAGTACGTGGTAATTA
CTTCGGTGGACCGCGATGACTTGCGTGATGGTGGTGCACAACATTTCGCCGATTGTAACCGTGAAATTCGCGCTTTGAAT
CCGCACATCAAGATTGAAACCTTAGTGCCTGATTTCCGTGGTCGTATGGAAGTGGCTCTTGAGGCCTTAAAAGACAATCC
ACCCGATGTGTTCAACCACAATTTGGAAACCGCACCACGCTTGTATCGTAAAGTGCGCCCAGGCGCTAACTATAAGTGGT
CGTTAGAACTACTGCGTCAATTTAAAGAGCAACATCCTCATGTACCGACTAAATCCGGTTTGATGATGGGTTTGGGTGAA
ACTAAAGAAGAGATCGTCGAAGTATTGAAAGATCTCCGTGCTCATGGCGTCACCATGCTGACTCTTGGCCAATATCTCGC
ACCAAGTCGCCACCATCTGCCGGTTGAACGCTACGTGCCACCCGCAGAGTTTGATGAGCTGAAAGAGATTGCTCTAGAAC
TCGGCTTTACTCATGCAGCCTGTGGACCCTTTGTACGTTCCTCTTACCATGCTGACTTACAAGCCAAGGGTTTAGAAGTC
AAATAA

Upstream 100 bases:

>100_bases
CATCCGGTCAGCATGGAACAGGTTGAAAAAGTATTAATCCAAGAACTCGTTACTCTACTGGACTACGAGCAAGTCGAATT
CAGCACAGAAGCATACAATC

Downstream 100 bases:

>100_bases
GCCTCCTCATCAAGCCTGCACCTCGCAGGCTTGATGCTTTTCACGGGGATGAGATCCTTCACCATAATCTCAACCAATTC
CCTGATTTAACCTTGAATCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIQMERGVKYRDADKMALIPIKNMPTEQKEVLRKPEWMKIKLPADSQRIQDIKAAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLAQTIHDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALN
PHIKIETLVPDFRGRMEVALEALKDNPPDVFNHNLETAPRLYRKVRPGANYKWSLELLRQFKEQHPHVPTKSGLMMGLGE
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAACGPFVRSSYHADLQAKGLEV
K

Sequences:

>Translated_321_residues
MSKPIQMERGVKYRDADKMALIPIKNMPTEQKEVLRKPEWMKIKLPADSQRIQDIKAAMRKNNLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLAQTIHDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALN
PHIKIETLVPDFRGRMEVALEALKDNPPDVFNHNLETAPRLYRKVRPGANYKWSLELLRQFKEQHPHVPTKSGLMMGLGE
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAACGPFVRSSYHADLQAKGLEV
K
>Mature_320_residues
SKPIQMERGVKYRDADKMALIPIKNMPTEQKEVLRKPEWMKIKLPADSQRIQDIKAAMRKNNLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLAQTIHDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALNP
HIKIETLVPDFRGRMEVALEALKDNPPDVFNHNLETAPRLYRKVRPGANYKWSLELLRQFKEQHPHVPTKSGLMMGLGET
KEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAACGPFVRSSYHADLQAKGLEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=44.7098976109215, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI37577164, Length=257, Percent_Identity=44.3579766536965, Blast_Score=214, Evalue=8e-56,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=81.3084112149533, Blast_Score=555, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=41.6083916083916, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=42.5742574257426, Blast_Score=241, Evalue=9e-65,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=46.1805555555556, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36469; Mature: 36337

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIQMERGVKYRDADKMALIPIKNMPTEQKEVLRKPEWMKIKLPADSQRIQDIKAAMR
CCCCCCHHCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIHDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALNPHIKIETLVPDFRGRMEVAL
HHHHHCEEEEEEEECCCHHHHHCCCHHHHHHHCHHHHHCCCCEEEEEECCCHHHHHHHHH
EALKDNPPDVFNHNLETAPRLYRKVRPGANYKWSLELLRQFKEQHPHVPTKSGLMMGLGE
HHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAA
CHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGLEVK
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIQMERGVKYRDADKMALIPIKNMPTEQKEVLRKPEWMKIKLPADSQRIQDIKAAMR
CCCCCHHCCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
KNNLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPNAPEAEEPKKLA
HCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIHDMKLKYVVITSVDRDDLRDGGAQHFADCNREIRALNPHIKIETLVPDFRGRMEVAL
HHHHHCEEEEEEEECCCHHHHHCCCHHHHHHHCHHHHHCCCCEEEEEECCCHHHHHHHHH
EALKDNPPDVFNHNLETAPRLYRKVRPGANYKWSLELLRQFKEQHPHVPTKSGLMMGLGE
HHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEECCCC
TKEEIVEVLKDLRAHGVTMLTLGQYLAPSRHHLPVERYVPPAEFDELKEIALELGFTHAA
CHHHHHHHHHHHHHCCEEHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCHHHH
CGPFVRSSYHADLQAKGLEVK
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]