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The map label for this gene is ispA [H]

Identifier: 227081066

GI number: 227081066

Start: 906875

End: 907759

Strand: Reverse

Name: ispA [H]

Synonym: VCM66_0847

Alternate gene names: 227081066

Gene position: 907759-906875 (Counterclockwise)

Preceding gene: 227081067

Following gene: 227081065

Centisome position: 31.38

GC content: 51.3

Gene sequence:

>885_bases
ATGATTGAGGCGTTATCCTCATACCAACAGCGAAATAACCAGCAACTGGATCAGTGGCTGAACCGTATTCCCTTTCAAAC
CTTGCCTTTAATCGAGGCGATGCGTTACGGGCTACTGCTCGGCGGCAAACGGGCACGGCCATATTTGGTGTACATCACGG
GGCAAATGCTCGGCTGTGAGCTTAGCGACTTAGACACTCCTGCCTCAGCCGTGGAGTGTATTCACGCCTATTCGCTGATC
CATGATGATTTGCCCGCCATGGATGATGATGAGCTGCGCCGCGGCAAGCCCACTTGCCATATCCAGTTTGATGAGGCCAC
CGCCATTTTGACCGGCGACGCCCTGCAAACCTTGGCGTTTACTATTTTGGCGGAAGGCGATCTTTCTGCTGCAGGCGAAA
CACAGCGCGTCGCCATGATCCAAGCTCTTGCCGAAGCTTCTGGCGCACAAGGCATGTGCCTTGGTCAAGCGTTAGATCTG
GCGGCGGAAAATCGCTTAATTTCGCTAGAGGAATTGGAAACCATTCATCGCAATAAAACCGGCGCTTTAATGCGCTGCGC
GATCCGTTTAGGTGCTTTAGCCGCAGGTGAAAAAGGCCGCGCCATGCTACCGCATCTTGATCGCTATGCAGAAGCGGTTG
GCCTCGCGTTTCAGGTTCAAGATGACATTCTCGATATCATTAGCGATACCGAAACCCTTGGTAAACCACAGGGTTCTGAC
CAAGAATTAAATAAAAGTACCTACCCTGCTCTTTTAGGCTTAGAAGGAGCACAACAAAAAGCACACACTCTGTTACAAGA
AGCGCTTCTGGCTTTAGAAGCAATTCCCTACAATACGGAGCATCTGGAAGAGTTCGCCCGATATGTCGTCGAGCGCAAGA
ACTAA

Upstream 100 bases:

>100_bases
TTTTGCTCAGTAACAGTGATGATGCCCCGCTCAGCGATTTTAGCGATGTAGCAGAGTAACTCGCTCCACAGCCAGAACAA
CCGACAAAGATGAGTGTCCT

Downstream 100 bases:

>100_bases
ACAATAAGCGCGCATACTTATGACTCTTGATATCTCAAAATACCCAACACTGGCTCTGGCGAATACGCCAGACGAACTGC
GCAGCCTTCCAAAAGAAGTA

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIEALSSYQQRNNQQLDQWLNRIPFQTLPLIEAMRYGLLLGGKRARPYLVYITGQMLGCELSDLDTPASAVECIHAYSLI
HDDLPAMDDDELRRGKPTCHIQFDEATAILTGDALQTLAFTILAEGDLSAAGETQRVAMIQALAEASGAQGMCLGQALDL
AAENRLISLEELETIHRNKTGALMRCAIRLGALAAGEKGRAMLPHLDRYAEAVGLAFQVQDDILDIISDTETLGKPQGSD
QELNKSTYPALLGLEGAQQKAHTLLQEALLALEAIPYNTEHLEEFARYVVERKN

Sequences:

>Translated_294_residues
MIEALSSYQQRNNQQLDQWLNRIPFQTLPLIEAMRYGLLLGGKRARPYLVYITGQMLGCELSDLDTPASAVECIHAYSLI
HDDLPAMDDDELRRGKPTCHIQFDEATAILTGDALQTLAFTILAEGDLSAAGETQRVAMIQALAEASGAQGMCLGQALDL
AAENRLISLEELETIHRNKTGALMRCAIRLGALAAGEKGRAMLPHLDRYAEAVGLAFQVQDDILDIISDTETLGKPQGSD
QELNKSTYPALLGLEGAQQKAHTLLQEALLALEAIPYNTEHLEEFARYVVERKN
>Mature_294_residues
MIEALSSYQQRNNQQLDQWLNRIPFQTLPLIEAMRYGLLLGGKRARPYLVYITGQMLGCELSDLDTPASAVECIHAYSLI
HDDLPAMDDDELRRGKPTCHIQFDEATAILTGDALQTLAFTILAEGDLSAAGETQRVAMIQALAEASGAQGMCLGQALDL
AAENRLISLEELETIHRNKTGALMRCAIRLGALAAGEKGRAMLPHLDRYAEAVGLAFQVQDDILDIISDTETLGKPQGSD
QELNKSTYPALLGLEGAQQKAHTLLQEALLALEAIPYNTEHLEEFARYVVERKN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=288, Percent_Identity=59.7222222222222, Blast_Score=332, Evalue=1e-92,
Organism=Escherichia coli, GI1789578, Length=218, Percent_Identity=32.5688073394495, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=191, Percent_Identity=26.7015706806283, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24651612, Length=185, Percent_Identity=29.7297297297297, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32260; Mature: 32260

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEALSSYQQRNNQQLDQWLNRIPFQTLPLIEAMRYGLLLGGKRARPYLVYITGQMLGCE
CCCHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
LSDLDTPASAVECIHAYSLIHDDLPAMDDDELRRGKPTCHIQFDEATAILTGDALQTLAF
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCEEEEEHHHHHHHHH
TILAEGDLSAAGETQRVAMIQALAEASGAQGMCLGQALDLAAENRLISLEELETIHRNKT
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCEECHHHHHHHHCCCC
GALMRCAIRLGALAAGEKGRAMLPHLDRYAEAVGLAFQVQDDILDIISDTETLGKPQGSD
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCC
QELNKSTYPALLGLEGAQQKAHTLLQEALLALEAIPYNTEHLEEFARYVVERKN
HHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEALSSYQQRNNQQLDQWLNRIPFQTLPLIEAMRYGLLLGGKRARPYLVYITGQMLGCE
CCCHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
LSDLDTPASAVECIHAYSLIHDDLPAMDDDELRRGKPTCHIQFDEATAILTGDALQTLAF
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCEEEEEHHHHHHHHH
TILAEGDLSAAGETQRVAMIQALAEASGAQGMCLGQALDLAAENRLISLEELETIHRNKT
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCEECHHHHHHHHCCCC
GALMRCAIRLGALAAGEKGRAMLPHLDRYAEAVGLAFQVQDDILDIISDTETLGKPQGSD
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCC
QELNKSTYPALLGLEGAQQKAHTLLQEALLALEAIPYNTEHLEEFARYVVERKN
HHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2089044; 9278503 [H]