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The map label for this gene is tcpA

Identifier: 227081005

GI number: 227081005

Start: 847901

End: 848575

Strand: Direct

Name: tcpA

Synonym: VCM66_0785

Alternate gene names: 227081005

Gene position: 847901-848575 (Clockwise)

Preceding gene: 227081004

Following gene: 227081006

Centisome position: 29.31

GC content: 43.26

Gene sequence:

>675_bases
ATGCAATTATTAAAACAGCTTTTTAAGAAGAAGTTTGTAAAAGAAGAACACGATAAGAAAACCGGTCAAGAGGGTATGAC
ATTACTCGAAGTAATCATTGTTCTGGGTATTATGGGTGTGGTCTCAGCGGGTGTTGTTACGCTGGCTCAGCGTGCGATTG
ATTCGCAGAATATGACTAAGGCTGCGCAAAATCTAAACAGCGTGCAAATTGCAATGACACAAACTTATCGTAGTCTTGGT
AATTATCCAGCTACCGCAAACGCAAATGCTGCTACACAGCTAGCTAATGGTTTGGTCAGCCTTGGTAAGGTTTCAGCTGA
TGAGGCAAAGAATCCTTTCACTGGTACAGCTATGGGGATTTTCTCATTTCCACGAAACTCTGCAGCGAATAAAGCATTCG
CAATTACAGTCGGTGGCTTGACCCAAGCACAATGTAAGACTTTGGTTACAAGCGTAGGGGATATGTTTCCATTTATCAAC
GTGAAAGAAGGTGCTTTCGCTGCTGTCGCTGATCTTGGTGATTTCGAAACGAGTGTCGCAGATGCTGCTACTGGCGCTGG
CGTAATTAAGTCCATTGCACCAGGAAGTGCCAACTTAAACCTAACTAATATCACGCATGTTGAGAAGCTTTGTACAGGAA
CTGCTCCATTCACAGTAGCTTTTGGTAACAGTTAA

Upstream 100 bases:

>100_bases
GTGACTTTGTGTGGTTAAATGTGCGTATTGCTTTCTTTTTCTTTAAAAAAGGACCAAGCAATGCATTTCCTTAAAACACA
AATGGTGGAGTTATATAAAT

Downstream 100 bases:

>100_bases
TCTACACCATTATCTTGATAATTTAATAATAATTTGCCCATGGAAATGGGCAAATTATTAAGCTAAAAGTGATATCAATA
TGAGAAAATACCAACAAGGT

Product: toxin co-regulated pilin

Products: NA

Alternate protein names: Pilus colonization factor

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQLLKQLFKKKFVKEEHDKKTGQEGMTLLEVIIVLGIMGVVSAGVVTLAQRAIDSQNMTKAAQNLNSVQIAMTQTYRSLG
NYPATANANAATQLANGLVSLGKVSADEAKNPFTGTAMGIFSFPRNSAANKAFAITVGGLTQAQCKTLVTSVGDMFPFIN
VKEGAFAAVADLGDFETSVADAATGAGVIKSIAPGSANLNLTNITHVEKLCTGTAPFTVAFGNS

Sequences:

>Translated_224_residues
MQLLKQLFKKKFVKEEHDKKTGQEGMTLLEVIIVLGIMGVVSAGVVTLAQRAIDSQNMTKAAQNLNSVQIAMTQTYRSLG
NYPATANANAATQLANGLVSLGKVSADEAKNPFTGTAMGIFSFPRNSAANKAFAITVGGLTQAQCKTLVTSVGDMFPFIN
VKEGAFAAVADLGDFETSVADAATGAGVIKSIAPGSANLNLTNITHVEKLCTGTAPFTVAFGNS
>Mature_224_residues
MQLLKQLFKKKFVKEEHDKKTGQEGMTLLEVIIVLGIMGVVSAGVVTLAQRAIDSQNMTKAAQNLNSVQIAMTQTYRSLG
NYPATANANAATQLANGLVSLGKVSADEAKNPFTGTAMGIFSFPRNSAANKAFAITVGGLTQAQCKTLVTSVGDMFPFIN
VKEGAFAAVADLGDFETSVADAATGAGVIKSIAPGSANLNLTNITHVEKLCTGTAPFTVAFGNS

Specific function: Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Fimbrium

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCPA1_VIBCH (Q60153)

Other databases:

- EMBL:   X74730
- EMBL:   U09807
- EMBL:   AE003852
- PIR:   JC4719
- RefSeq:   NP_230476.1
- PDB:   3HRV
- PDBsum:   3HRV
- ProteinModelPortal:   Q60153
- SMR:   Q60153
- GeneID:   2614495
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0828
- TIGR:   VC_0828
- HOGENOM:   HBG739727
- OMA:   HISALCG
- ProtClustDB:   CLSK793805
- PMAP-CutDB:   Q60153
- GO:   GO:0009289
- GO:   GO:0009405
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR010271
- TIGRFAMs:   TIGR02532

Pfam domain/function: PF05946 TcpA

EC number: NA

Molecular weight: Translated: 23279; Mature: 23279

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLKQLFKKKFVKEEHDKKTGQEGMTLLEVIIVLGIMGVVSAGVVTLAQRAIDSQNMTK
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
AAQNLNSVQIAMTQTYRSLGNYPATANANAATQLANGLVSLGKVSADEAKNPFTGTAMGI
HHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHH
FSFPRNSAANKAFAITVGGLTQAQCKTLVTSVGDMFPFINVKEGAFAAVADLGDFETSVA
HCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHCHHHHHHH
DAATGAGVIKSIAPGSANLNLTNITHVEKLCTGTAPFTVAFGNS
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MQLLKQLFKKKFVKEEHDKKTGQEGMTLLEVIIVLGIMGVVSAGVVTLAQRAIDSQNMTK
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
AAQNLNSVQIAMTQTYRSLGNYPATANANAATQLANGLVSLGKVSADEAKNPFTGTAMGI
HHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHH
FSFPRNSAANKAFAITVGGLTQAQCKTLVTSVGDMFPFINVKEGAFAAVADLGDFETSVA
HCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHCHHHHHHH
DAATGAGVIKSIAPGSANLNLTNITHVEKLCTGTAPFTVAFGNS
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8621096; 7854116; 10952301