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The map label for this gene is tcpI [H]

Identifier: 227081002

GI number: 227081002

Start: 843996

End: 845858

Strand: Reverse

Name: tcpI [H]

Synonym: VCM66_0782

Alternate gene names: 227081002

Gene position: 845858-843996 (Counterclockwise)

Preceding gene: 227081021

Following gene: 227080997

Centisome position: 29.24

GC content: 38.11

Gene sequence:

>1863_bases
ATGATAAAAAAAATAATTTCGGTTTTTTTATTACTTGCGTGCATCATTACGTTGGCTTTTACTGCCTTTTTCTATCACTC
GAAACTATCAGATCAAACAAAATCTATATCTAGCCTTAGTTCTCAGCAGGCACAAGAACGGTTGCAAAGTTATCAGGATT
CTTTAGATTTTTATAAAAAACTTAATACCTCTCTCTCAGTTGCAATAGCCAATTCACTTCGCGATAAAGCAGTCGAAGAA
CTCAACGCTATTGCTTTGCGTATACAGGAAAATCATGGGTTTATTGGTGTTACCTTCGCTAGTTTAGATGGCACCATGTT
TACAGATATTGGTACTCTAGATTGGAATGCAAAGACATTGAGAAGAGATTGGTTTGTTAAAACGGTCGAACTCGGTACTA
AACATTACACAGCTTTTGATATCGATAAAACAACTGGGCAACACGTTCTCACTATTGCCACCCCTGTTTATGTAGGCAAC
GACATTGTCGGCAGTGTCGCTTTAGATATTGCAGGTGATCAGATTGCCTCACCTAACGGCAGTGGGATGTTTATGATGAC
GGATAGGAATTTTAATGTATTTGCATCTGATCTCACTCACAGTACCTTAATTGGAAAAGATTTAACGAAAGAGAAGCCTC
TGTTCAAAAATCTGGTCTCAGGCCAGTATGTCACCTTCAGTGATGCTGATAGTCATTGGTTTGCAGTGTCACAAACAGAA
ATAGATGGTGAAAATAAACTGTTCACTATTATTGATATTCAACAAATCGTCCAAACATACAAGCGAGATATCCAGTTGAT
TATTGCTGGATTTAGTGGTTTTTCATGCGTGATGCTAATTGGACTATATTGGGTTCTAAGCAAAGAACTCTCAGGAGTTA
GACAGATCAGAGAATGGATTCTTGCCCTTTCTGATGGTCAGATAAAAGAACGCAGGCCTATAAAATTCCATAACGAGCTC
GACACTATTGCCCAAAGTCTTGAAAATTTACAATTTAGGCTTCTTGATGTGGTGAGAAATTCTCATCGGACTATGAATGA
TTTAAGTATCAAACAGACTGACATTACGTATTCAATTGAAGGGAATACGAATAATTCACAGCAAGAACTTGGGCTCATTG
AACAAGTGGCCACAGCAACAACGCAACTATCATGTACTTCTTTTGATGTAATGCAACAAGCTCAAAGTGCAGAGCTTAAT
GCGGAGACGGCCCAAAAATTGATTGCTGAAAGTCATGATATTATCGATTCATCAAGCAAACAAACCGAAATGGTAACCCT
ATCGATCCATGAATCCCAACAAATAATCAATCAGTTACGCGAGTTTTCCGATAATATTAGTTCGGTTACGGATGTCATTA
ATAATATATCAGATCAAACTAACTTACTTGCCCTAAATGCAGCTATCGAGGCTGCTCGTGCAGGTGAACAGGGGCGCGGA
TTTGCTGTAGTAGCGGATGAGGTTCGTTCTCTTGCAGTGAAAACTCAGCAATCGACTATAGATATTCAAGGTATTATTTT
GAAACTGCAAGAGCAATCGCAGTTGGCAGATCAAGTGATGACACGTAATGTTTCACTTATTCATGAAACACAAGTTGCCA
ATCGGGCACTTATCGCATCCTTTAACCTGATTTCTGATAAGGTTCTTGAAATATCAAACATAAACTCCATCGTTTCGACT
GCCGCAAATGAACAAAAAATAGTAACTGAAGATGTAGCAAAACAGATGGAAGATATTCGATATCTAGTTCAAGAGAATTT
ATCCGCGATGGAACGAACTAAACAGGCAAATCAGAATATCTCTGATTTGACCACCAATCTAAATGATGCTCTTAGCTTTT
TTAAAATTGAGCTTACTTCATAA

Upstream 100 bases:

>100_bases
CGATAAAAGCTGATTTTTCACATCATTATTTTATAATTTCATTTTTAACTAATTATCACGAAGTACTCCGTGATAACCAT
TTAAACTTAGAGTTAAACTA

Downstream 100 bases:

>100_bases
ATTAGGCTAGTGCCAGTCAGTTGAGCTGACTGGCTTTTTTTCTCGCACAATCGTGTAGCCATGCTACAACAACCTTTACA
CATGTTAGCTATACCAAAGT

Product: toxin co-regulated pilus biosynthesis protein I

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpI [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MIKKIISVFLLLACIITLAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKKLNTSLSVAIANSLRDKAVEE
LNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWILALSDGQIKERRPIKFHNEL
DTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELN
AETAQKLIAESHDIIDSSSKQTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIASFNLISDKVLEISNINSIVST
AANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNISDLTTNLNDALSFFKIELTS

Sequences:

>Translated_620_residues
MIKKIISVFLLLACIITLAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKKLNTSLSVAIANSLRDKAVEE
LNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWILALSDGQIKERRPIKFHNEL
DTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELN
AETAQKLIAESHDIIDSSSKQTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIASFNLISDKVLEISNINSIVST
AANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNISDLTTNLNDALSFFKIELTS
>Mature_620_residues
MIKKIISVFLLLACIITLAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKKLNTSLSVAIANSLRDKAVEE
LNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTLRRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGN
DIVGSVALDIAGDQIASPNGSGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWILALSDGQIKERRPIKFHNEL
DTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIEGNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELN
AETAQKLIAESHDIIDSSSKQTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIASFNLISDKVLEISNINSIVST
AANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNISDLTTNLNDALSFFKIELTS

Specific function: May function as an environmental regulator of TCP biogenesis. Negatively regulates the synthesis of the major pilin subunit of TCP (tcpA) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=312, Percent_Identity=30.4487179487179, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787690, Length=310, Percent_Identity=30, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788195, Length=337, Percent_Identity=28.486646884273, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI2367378, Length=309, Percent_Identity=28.4789644012945, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789453, Length=282, Percent_Identity=26.9503546099291, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004089 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 69029; Mature: 69029

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIISVFLLLACIITLAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNTSLSVAIANSLRDKAVEELNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTL
HCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCEEEEECCCCCCCHHHH
RRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGNDIVGSVALDIAGDQIASPNG
HHHHHHEEEECCCCEEEEEECCCCCCCEEEEEECCEEECCCHHHHHEEEECCCCCCCCCC
SGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
CEEEEEECCCCEEEEHHCHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCCCCEEEEEEEE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWI
ECCCCCEEEEEEHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSDGQIKERRPIKFHNELDTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIE
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEEEEE
GNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELNAETAQKLIAESHDIIDSSSK
CCCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
QTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCC
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIAS
EEEEHHHHHHHHHHHCCCEEEEEHEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FNLISDKVLEISNINSIVSTAANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SDLTTNLNDALSFFKIELTS
HHHHCCHHHHHEEEEEEECC
>Mature Secondary Structure
MIKKIISVFLLLACIITLAFTAFFYHSKLSDQTKSISSLSSQQAQERLQSYQDSLDFYKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNTSLSVAIANSLRDKAVEELNAIALRIQENHGFIGVTFASLDGTMFTDIGTLDWNAKTL
HCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEEECCCEEEEECCCCCCCHHHH
RRDWFVKTVELGTKHYTAFDIDKTTGQHVLTIATPVYVGNDIVGSVALDIAGDQIASPNG
HHHHHHEEEECCCCEEEEEECCCCCCCEEEEEECCEEECCCHHHHHEEEECCCCCCCCCC
SGMFMMTDRNFNVFASDLTHSTLIGKDLTKEKPLFKNLVSGQYVTFSDADSHWFAVSQTE
CEEEEEECCCCEEEEHHCHHHHHHCCCCCCCCCHHHHHHCCCEEEEECCCCCEEEEEEEE
IDGENKLFTIIDIQQIVQTYKRDIQLIIAGFSGFSCVMLIGLYWVLSKELSGVRQIREWI
ECCCCCEEEEEEHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSDGQIKERRPIKFHNELDTIAQSLENLQFRLLDVVRNSHRTMNDLSIKQTDITYSIE
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEEEEE
GNTNNSQQELGLIEQVATATTQLSCTSFDVMQQAQSAELNAETAQKLIAESHDIIDSSSK
CCCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
QTEMVTLSIHESQQIINQLREFSDNISSVTDVINNISDQTNLLALNAAIEAARAGEQGRG
CEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCC
FAVVADEVRSLAVKTQQSTIDIQGIILKLQEQSQLADQVMTRNVSLIHETQVANRALIAS
EEEEHHHHHHHHHHHCCCEEEEEHEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FNLISDKVLEISNINSIVSTAANEQKIVTEDVAKQMEDIRYLVQENLSAMERTKQANQNI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SDLTTNLNDALSFFKIELTS
HHHHCCHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8005659 [H]