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The map label for this gene is tpx [H]

Identifier: 227081001

GI number: 227081001

Start: 843226

End: 843720

Strand: Direct

Name: tpx [H]

Synonym: VCM66_0781

Alternate gene names: 227081001

Gene position: 843226-843720 (Clockwise)

Preceding gene: 227081000

Following gene: 227081003

Centisome position: 29.15

GC content: 39.19

Gene sequence:

>495_bases
ATGACTGTTACATTTCAAAATAATCCTGTTTCTATTTCTGGCTCTTTTCCTAAAGTAGGTGATCGTTTGCCTAGTTTTAC
TCTTTGTGGTGCTGATTTGAACGATCTTAGCAATGAAGATTTTAAAGGAAAAAAGATTGTAATGAGCATTTTCCCCAGTA
TCGACACTCCGGTTTGTTCTAAAAGTGTGAAAGTTCTTCAAAATGCACTTATGACTCGCAATGACACAGTGCTTCTCTGT
GTTTCAGCAGATCTGCCTTTTGCTATGTCTCGCTTTTGTACAGAGCATGCAGTAGCTAATGTCACGAATGCGTCGTTTTT
TCGTGAGCCTGCTTTTACTGAACGCTTCGGTGTAAATCTAAATGAAGGTGCTTTACGTGGGCTTGCTGCTAGAGCAGTGA
TTGTTGCAGATGAGTTCGGTGTAATTACCCACAGTGAATTAGTCAATGAAATCACCAATGAACCAGATTACGATCGTATT
TTGATGTCACTATAA

Upstream 100 bases:

>100_bases
AAGTTCTAAACAGATTTTTTTCATATAGTTTGGTTTGTTTTTAATAGTTTGTATAATAGCGGTGACACTAAAGTAGTGTT
TGTATTTAAGGAATTAAATT

Downstream 100 bases:

>100_bases
TCAGTAATTAGGCCAATTTTATCTATCAATAGATAAAAAAGCGTTGGATGACTTTATAAAAGGCTCTAAGTTCCCAAGGC
TTAGAGCCTTTTATTTTTCA

Product: thiol peroxidase

Products: oxidized thioredoxin; H2O [C]

Alternate protein names: Protein tagD [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTVTFQNNPVSISGSFPKVGDRLPSFTLCGADLNDLSNEDFKGKKIVMSIFPSIDTPVCSKSVKVLQNALMTRNDTVLLC
VSADLPFAMSRFCTEHAVANVTNASFFREPAFTERFGVNLNEGALRGLAARAVIVADEFGVITHSELVNEITNEPDYDRI
LMSL

Sequences:

>Translated_164_residues
MTVTFQNNPVSISGSFPKVGDRLPSFTLCGADLNDLSNEDFKGKKIVMSIFPSIDTPVCSKSVKVLQNALMTRNDTVLLC
VSADLPFAMSRFCTEHAVANVTNASFFREPAFTERFGVNLNEGALRGLAARAVIVADEFGVITHSELVNEITNEPDYDRI
LMSL
>Mature_163_residues
TVTFQNNPVSISGSFPKVGDRLPSFTLCGADLNDLSNEDFKGKKIVMSIFPSIDTPVCSKSVKVLQNALMTRNDTVLLCV
SADLPFAMSRFCTEHAVANVTNASFFREPAFTERFGVNLNEGALRGLAARAVIVADEFGVITHSELVNEITNEPDYDRIL
MSL

Specific function: Has antioxidant activity. Could remove peroxides or H(2)O(2). Could play a role in fimbria synthesis or assembly [H]

COG id: COG2077

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1787584, Length=164, Percent_Identity=47.5609756097561, Blast_Score=158, Evalue=2e-40,

Paralogues:

None

Copy number: 2480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018219
- InterPro:   IPR002065
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: 1.11.1.- [C]

Molecular weight: Translated: 17925; Mature: 17793

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01265 TPX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTFQNNPVSISGSFPKVGDRLPSFTLCGADLNDLSNEDFKGKKIVMSIFPSIDTPVCS
CEEEECCCCEEEECCCCCHHHCCCCEEEECCCHHHCCCCCCCCCEEEEEECCCCCCCHHH
KSVKVLQNALMTRNDTVLLCVSADLPFAMSRFCTEHAVANVTNASFFREPAFTERFGVNL
HHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHCCCC
NEGALRGLAARAVIVADEFGVITHSELVNEITNEPDYDRILMSL
CHHHHHHHHHEEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure 
TVTFQNNPVSISGSFPKVGDRLPSFTLCGADLNDLSNEDFKGKKIVMSIFPSIDTPVCS
EEEECCCCEEEECCCCCHHHCCCCEEEECCCHHHCCCCCCCCCEEEEEECCCCCCCHHH
KSVKVLQNALMTRNDTVLLCVSADLPFAMSRFCTEHAVANVTNASFFREPAFTERFGVNL
HHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHCCCC
NEGALRGLAARAVIVADEFGVITHSELVNEITNEPDYDRILMSL
CHHHHHHHHHEEEEEECCCCCEEHHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced thioredoxin; H2O2 [C]

Specific reaction: reduced thioredoxin + H2O2 = oxidized thioredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7523254 [H]