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The map label for this gene is citS [H]

Identifier: 227080974

GI number: 227080974

Start: 809009

End: 810355

Strand: Reverse

Name: citS [H]

Synonym: VCM66_0753

Alternate gene names: 227080974

Gene position: 810355-809009 (Counterclockwise)

Preceding gene: 227080983

Following gene: 227080973

Centisome position: 28.02

GC content: 49.29

Gene sequence:

>1347_bases
ATGAACGAGAAAACCCTACATTTAGATAAAAAGTCCGCAGTCGTAGGTGGATTGAATCTAAGCCAAACGAAAATATTTGG
CACTCCACTCCCTTTATTTTTATTTTTACTGCTCACCGTATTAGCCGCTCATTTTACTGACACTTTACCTAGCGGTCTGG
TGGGTGGTTTTGCGTTTATGTTTGTCATCGGCGCAATATTTGGCGAAGTTGGCAAACGTCTACCGATTTTTAATAAATAC
ATCGGCGGTGCGCCGGTAATGATTTTCTTGGTGGCCGCTTGGTTCGTGCACATGGGCTGGCTCAGTGATCGCGAAATCAA
AACCGTGACCGAAGTGATGGATAAGAGCAAATTCTTGGATCTGTTCATCGCAGTGCTGATCACTGGCTCAATTCTGGCGG
TCAACCGTAAACTGCTGATCCGCTCTCTGGCTGGCTACATTCCAACTATTCTCGCTGCGGTAGCGGGTGCTGCGGTGTTC
GGTATCGCTATCGGCCTCGTATTTGGTATCACGCCTGATCGCATCATGATGCTGTACGTGCTACCGATTATGGGCGGTGG
TAATGGCGCAGGCGCTATCCCGCTTTCCGAAATTTATGAATCGGTCACCGGTGGCTCGAAAGAGCAATACTACTCGGTTG
CTATCGCGATCCTGACTATCGCCAACATTGTGGCCATCGTCGCCGCGGCAGTCATCAACATCATTGGTGAAAAGATCCCA
TCCTTAACCGGCAATGGCGAACTGGTACGCAGCTCGAAACTGGATGTGAGCGAAGAAGCGAAAAACCTGACAGTGACTCA
CCGTGAAATTGCCATCGGTTTAATGCTGGCCGCTTGTGTGTACACCTTCTCTTATGCGCTATCGAAAGAGATTTTGCCCG
GTTTTGGTTCGATTCAAATCCACACCTTTGCTTACATGGTGGTGATCATCGCCATCCTTAACGCGGCGGGTTTGTGCAGT
GATGAAATCAAAGAAGGCGCGAAACGTCTATCGAATTTCTTCTCAAAACAGATGCTGTGGTTACTGATGGTCGGCGTGGG
TATTGCTTACACCGATCTGGCTGAAATCATCAACGCGCTAACTTTTGCCAATGTAATCATTGCTACGGTGATTGTGCTTG
GTGCGATTATCGGTGCTGCTTTAGGTGGCTGGGCGATGGGCTTCTACCCTGTTGAAGCTTCGATTACAGCAGGGCTATGT
ATGGCTAACCGCGGCGGCTCGGGCGACCTTGAAGTGCTTGCCGCCTCTAACCGTATGAGCTTGCTCTCTTACGCACAAAT
ATCTTCACGTCTGGGTGGCGGCATTGTGCTGGTCATCGCCAGCGTCGTATTCGGAATGTTTGTTTAA

Upstream 100 bases:

>100_bases
ATTTTTAAAGCCACTGGTTTGGATGACTATTTTCACAATTATCACTGACTATTTTATTTAATGTAGTAAAGGTCTTAATA
ACCTCATTGGACATGGAATT

Downstream 100 bases:

>100_bases
CCCTTTAACCCTTGCTCCTTGGGATTATTTGGCGCGCTTAGCGCGCCTTTTTTTCTCTCCGTTTTCAACCCTGTTTTTAC
CACTGGAAGTGACCATGCAA

Product: citrate/sodium symporter

Products: NA

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNEKTLHLDKKSAVVGGLNLSQTKIFGTPLPLFLFLLLTVLAAHFTDTLPSGLVGGFAFMFVIGAIFGEVGKRLPIFNKY
IGGAPVMIFLVAAWFVHMGWLSDREIKTVTEVMDKSKFLDLFIAVLITGSILAVNRKLLIRSLAGYIPTILAAVAGAAVF
GIAIGLVFGITPDRIMMLYVLPIMGGGNGAGAIPLSEIYESVTGGSKEQYYSVAIAILTIANIVAIVAAAVINIIGEKIP
SLTGNGELVRSSKLDVSEEAKNLTVTHREIAIGLMLAACVYTFSYALSKEILPGFGSIQIHTFAYMVVIIAILNAAGLCS
DEIKEGAKRLSNFFSKQMLWLLMVGVGIAYTDLAEIINALTFANVIIATVIVLGAIIGAALGGWAMGFYPVEASITAGLC
MANRGGSGDLEVLAASNRMSLLSYAQISSRLGGGIVLVIASVVFGMFV

Sequences:

>Translated_448_residues
MNEKTLHLDKKSAVVGGLNLSQTKIFGTPLPLFLFLLLTVLAAHFTDTLPSGLVGGFAFMFVIGAIFGEVGKRLPIFNKY
IGGAPVMIFLVAAWFVHMGWLSDREIKTVTEVMDKSKFLDLFIAVLITGSILAVNRKLLIRSLAGYIPTILAAVAGAAVF
GIAIGLVFGITPDRIMMLYVLPIMGGGNGAGAIPLSEIYESVTGGSKEQYYSVAIAILTIANIVAIVAAAVINIIGEKIP
SLTGNGELVRSSKLDVSEEAKNLTVTHREIAIGLMLAACVYTFSYALSKEILPGFGSIQIHTFAYMVVIIAILNAAGLCS
DEIKEGAKRLSNFFSKQMLWLLMVGVGIAYTDLAEIINALTFANVIIATVIVLGAIIGAALGGWAMGFYPVEASITAGLC
MANRGGSGDLEVLAASNRMSLLSYAQISSRLGGGIVLVIASVVFGMFV
>Mature_448_residues
MNEKTLHLDKKSAVVGGLNLSQTKIFGTPLPLFLFLLLTVLAAHFTDTLPSGLVGGFAFMFVIGAIFGEVGKRLPIFNKY
IGGAPVMIFLVAAWFVHMGWLSDREIKTVTEVMDKSKFLDLFIAVLITGSILAVNRKLLIRSLAGYIPTILAAVAGAAVF
GIAIGLVFGITPDRIMMLYVLPIMGGGNGAGAIPLSEIYESVTGGSKEQYYSVAIAILTIANIVAIVAAAVINIIGEKIP
SLTGNGELVRSSKLDVSEEAKNLTVTHREIAIGLMLAACVYTFSYALSKEILPGFGSIQIHTFAYMVVIIAILNAAGLCS
DEIKEGAKRLSNFFSKQMLWLLMVGVGIAYTDLAEIINALTFANVIIATVIVLGAIIGAALGGWAMGFYPVEASITAGLC
MANRGGSGDLEVLAASNRMSLLSYAQISSRLGGGIVLVIASVVFGMFV

Specific function: Uptake of citrate across the boundary membrane with the concomitant uptake of a sodium ion (symport system) [H]

COG id: COG3493

COG function: function code C; Na+/citrate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:citrate (SCF) symporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004679
- InterPro:   IPR018025 [H]

Pfam domain/function: PF03390 2HCT [H]

EC number: NA

Molecular weight: Translated: 47284; Mature: 47284

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1577734; 8810332 [H]