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The map label for this gene is rlmN [H]

Identifier: 227080936

GI number: 227080936

Start: 766017

End: 767138

Strand: Direct

Name: rlmN [H]

Synonym: VCM66_0715

Alternate gene names: 227080936

Gene position: 766017-767138 (Clockwise)

Preceding gene: 227080935

Following gene: 227080937

Centisome position: 26.48

GC content: 47.95

Gene sequence:

>1122_bases
ATGACCACAGAAAAAGTCAATCTACTCGATTTTGACCGCAAGGGTTTGCGCACGTTTTTTGCCGAAGAACTGGGTGAGAA
AGCGTTTCGTGCCGAGCAGGTCATGAAGTGGATTTATCACTTTGGTTGTGATGACTTCGATCAAATGAATAACATCAACA
AGCAGCTGCGTGAAAAGCTCAAAGCCAAGTGTGAGATCCGTGCACCTTATGTCTCGGAAGCGCAACACTCGGCGGATGGC
ACCATCAAGTGGGCAATGCGTGTGGGTGATCAGGATGTAGAAACGGTTTACATTCCCGAAGATGATCGCGCGACCTTATG
TGTATCTTCCCAAGTAGGGTGTGCGTTGGAATGCAAATTCTGCTCAACGGCGCAGCAAGGTTTTAACCGTAACCTACGCG
TTTCAGAAATCATTGGTCAGGTTTGGCGTGCGGCACGTGAAATCGGTCTAGAGAAAGAAACTGGGCGTCGTCCTATCACT
AACGTGGTGATGATGGGCATGGGTGAGCCTTTGCTCAACATGAAAAACCTCATCCCAGCGTTAGAGATCATGCTCGATGA
TCTCGGTTTTGGTCTTTCTAAGCGCCGTGTGACCGTTTCTACCTCTGGTGTGGTTTCTGGTTTAGAGCAGATGATTGGCC
AAATCGATGTGGCTTTGGCTATCTCATTGCATGCGCCGAATGACAAGCTGCGTAGCGAAATCATGCCGATCAACGATCGC
TGGAATATCGAAGCGTTTTTAGAGGTGGTGCGTCGCTATATTGCATCCTCAAATGCTAACCGCGGTAAGGTGACCGTTGA
GTATGTGCTGCTGGATCATGTGAACGATGGTACAGAGCATGCTCACGAGTTGGCGGAATTACTGAAAGGGACGCCTTGTA
AAATTAACCTGATCCCGTTTAACCCTTATCCGGGCTCACCCTACAAGAAGCCAAGTAATTCACGCATCGACCGTTTCCAG
AAAACCTTGATGCAATATGAGCATACCGTCACCATTCGTAAAACTCGTGGTGATGACATTGATGCGGCATGTGGGCAGTT
GGTGGGAGATGTCATTGATCGTACTAAACGAACCAAAGCCAAACAATTTGATGGACAAGCGATTCCAGTGAAAACACTGT
AA

Upstream 100 bases:

>100_bases
ACACCTTAGAAAAGACGCGGTTTTGCAGCCCTTGTTGTTGCTGAATAAAGGCTGTACAATTCGCGCCCTTAATTACTGTT
CCGTCTTCCTGAGAGGCCCC

Downstream 100 bases:

>100_bases
TTGATGTTATAAGATAAGCCAGAGATGTTTCTCTGGCTTTATTTTTGCCTAATATGGCAACAAATCACTGCGTTTAAATG
TCAAATCAAAGAAAAACCTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKLKAKCEIRAPYVSEAQHSADG
TIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKGTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKAKQFDGQAIPVKTL

Sequences:

>Translated_373_residues
MTTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKLKAKCEIRAPYVSEAQHSADG
TIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKGTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKAKQFDGQAIPVKTL
>Mature_372_residues
TTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKLKAKCEIRAPYVSEAQHSADGT
IKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITN
VVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDRW
NIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKGTPCKINLIPFNPYPGSPYKKPSNSRIDRFQK
TLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKAKQFDGQAIPVKTL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=369, Percent_Identity=73.4417344173442, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42081; Mature: 41950

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKL
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KAKCEIRAPYVSEAQHSADGTIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKF
HHCCEECCCCHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCC
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKGTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQFDGQAIPVKTL
HHCCCCCCCCCCC
>Mature Secondary Structure 
TTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKL
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KAKCEIRAPYVSEAQHSADGTIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKF
HHCCEECCCCHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCC
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKGTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQFDGQAIPVKTL
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA