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The map label for this gene is tgt [H]

Identifier: 227080920

GI number: 227080920

Start: 751691

End: 752830

Strand: Direct

Name: tgt [H]

Synonym: VCM66_0699

Alternate gene names: 227080920

Gene position: 751691-752830 (Clockwise)

Preceding gene: 227080918

Following gene: 227080921

Centisome position: 25.99

GC content: 47.54

Gene sequence:

>1140_bases
GTGAAATTAAAATTTGAACTGAAAAAGAAAAACGGTAATGCCCGTCGTGGTCAATTGATTTTTGAACGCGGTACAGTGCA
AACCCCTGCGTTTATGCCCGTGGGTACTTACGGCACGGTAAAAGGGATGACCCCTGAAGAAGTGAAAGAGACCGGCGCAC
AAATTCTGCTCGGTAACACTTTCCACCTCTGGCTGCGCCCTGGCCAAGAAGTGATGAAAATGCACGGTGACTTGCACGAT
TTCATGAACTGGCAAGGTCCTATCCTGACTGACTCTGGTGGTTTTCAGGTATTCAGCTTAGGCGATATCCGTAAGATCAC
CGAAGAAGGCGTGCATTTCCGCAACCCAGTAAACGGCGACAAAATTTTTATGGACGCCGAGAAATCGATGGAAATCCAAA
AAGATTTGGGTTCAGACATCGTGATGATTTTTGACGAGTGTACGCCATACCCAGCGACCCACGATGAAGCGAAAAAATCG
ATGGAAATGTCTCTGCGTTGGGCAAAACGTTCGCGTGACCACTTCGATAAGCTCGAAAACCCGAACAACTTGTTTGGTAT
CGTGCAAGGTGGTGTGTATGAAGATTTGCGTGATGTATCGGTAAAAGGCCTGACGGAAATCGGTTTTGATGGTTATGCTG
TGGGTGGTCTTGCGGTGGGTGAGCCAAAAGAAGACATGCATCGTGTACTGGAACATACCTGTCCACAACTACCTGAAGAC
AAGCCACGTTATCTGATGGGCGTCGGTAAACCGGAAGACTTGGTGGAAGGTGTGCGTCGTGGTATCGACATGTTTGACTG
TGTGATGCCAACGCGTAACGCACGTAACGGTCACCTATTTGTGACGGGTGGTGTGATCAAGATCCGTAATGCAGCACATA
AAACCGATACAACACCACTGGATCCTCATTGCGACTGTTACACTTGCAAAAATTATTCGAAGTCATACCTGCATCATCTG
GATCGCTGTAACGAAATCCTCGGTGCACGCTTGAATACTATCCATAACCTGCGTTACTACCAACGCTTGATGGAAAGCAT
TCGTAAAGCGATTGATGAAGACCGTTTTGACCAATTTGTAGCCGAGTTCTACGCGCGTCGTAACCGCGAAGTGCCACCAC
TACAAAAAGACAAAGCCTGA

Upstream 100 bases:

>100_bases
GATGGCAATAAGGCTTGAATTATCGACCTGCTGAGAGCGTAAGTTTGCGCGATTAGGTGGGTAAAAATGGTCAGACTGTT
TCTCTGACATTGGAGGCTTC

Downstream 100 bases:

>100_bases
TTTCGTGCACTGGGTTGGATTTGCGTGGTAAGCCGCTTGAATTCACCCCTGTGCATCCCAATAGAATCAACATTAAACAA
CAATAACTTAGAGGACGTTT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEVMKMHGDLHD
FMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKS
MEMSLRWAKRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPLDPHCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFVAEFYARRNREVPPLQKDKA

Sequences:

>Translated_379_residues
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEVMKMHGDLHD
FMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKS
MEMSLRWAKRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPLDPHCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFVAEFYARRNREVPPLQKDKA
>Mature_379_residues
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEVMKMHGDLHD
FMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKS
MEMSLRWAKRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPLDPHCDCYTCKNYSKSYLHHL
DRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFVAEFYARRNREVPPLQKDKA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=43.2506887052342, Blast_Score=311, Evalue=5e-85,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=81.0666666666667, Blast_Score=660, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.4364640883978, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=163, Percent_Identity=33.7423312883436, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=46.2603878116344, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=28.515625, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43254; Mature: 43254

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNT
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCE
FHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGD
EEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCC
KIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKSMEMSLRWAKRSRDHFDKLEN
EEEEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
CCCEEEEECCCHHHHHHHHHHCCHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCC
DPHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFV
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEFYARRNREVPPLQKDKA
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLKFELKKKNGNARRGQLIFERGTVQTPAFMPVGTYGTVKGMTPEEVKETGAQILLGNT
CCEEEEEECCCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCE
FHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGD
EEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCC
KIFMDAEKSMEIQKDLGSDIVMIFDECTPYPATHDEAKKSMEMSLRWAKRSRDHFDKLEN
EEEEECHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PNNLFGIVQGGVYEDLRDVSVKGLTEIGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPED
CCCEEEEECCCHHHHHHHHHHCCHHHCCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCC
KPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVIKIRNAAHKTDTTPL
CCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCC
DPHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFDQFV
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AEFYARRNREVPPLQKDKA
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA