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The map label for this gene is queA [H]

Identifier: 227080918

GI number: 227080918

Start: 750404

End: 751462

Strand: Direct

Name: queA [H]

Synonym: VCM66_0697

Alternate gene names: 227080918

Gene position: 750404-751462 (Clockwise)

Preceding gene: 227080917

Following gene: 227080920

Centisome position: 25.94

GC content: 50.05

Gene sequence:

>1059_bases
ATGCAAGTTTCAGATTTTCATTTTGAACTCCCAGACGAGCTGATTGCTCGCTACCCAAAAGCAGAACGTACCGCGAGCCG
CTTGCTGCAGCTTGACGGCAACAGTGGTCAACTGGTGGATGGCACCTTTAAAGATGTCCTCGAACTGGTTGAACCGGGAG
ATCTGCTGGTGTTTAACAACACGCGAGTGATCCCAGCACGCATGTTTGGGCGCAAAGCCAGCGGTGGCAAATTAGAAGTG
TTGGTAGAGCGTATGCTCGATGAGCACACGATTTTAGCGCACGTTCGTTCATCAAAACCGCCCAAACCGGGTACTGAACT
TTATTTAGGCGAGAACGATGAGTTTCATGCCGTGATGCAGGCGCGTCACGATGCATTGTTTGAAATCCGTTTTACTGCGG
AAACCGTGGTATTGGATATTCTCAATCAAATTGGTCACATGCCGTTGCCACCTTACATCGACCGTCCTGATGAAGAAGCG
GATAAAGAGCGTTATCAAACGGTGTACAACCAAAAGCCGGGTGCGGTAGCTGCCCCAACGGCGGGTTTGCATTTTGACCA
AGCGCTGCTTGAGCAGATCCAAGCCAAAGGCGTTGAGTTGGCGTATGTAACACTGCATGTTGGCGCGGGGACCTTCCAGC
CTGTGCGGGTGGAAAATATTCACGATCACCATATGCATGCGGAATACGTTGAAGTGCCACAAGAAGTGGTGGATGCGATT
ACGGCGACCAAAGCACGTGGTGGTCGAGTGGTTGCTGTGGGTACCACCTCAGTACGTTCACTCGAAAGTGCGGCGCAAGA
TGCGCTGCAAAAAGGCACCGAGTTAAAGCCATTTTTTGGTGATACCGAAATCTTCATCTTCCCGGGGTATCAATACCAGT
TGGTGGATTGCTTGATCACCAATTTCCACTTGCCTGAATCGACCTTGATCATGCTGGTGAGTGCGTTTGCAGGTTATGAG
CACACCATGGCCGCTTATGAACATGCGGTCAAAGAGCAATATCGCTTCTTTAGTTACGGCGATGCGATGTTTATTCGTAA
GCAGATGACTAAAGCGTAA

Upstream 100 bases:

>100_bases
TATCACCACGCAGCATTCCTGTATCTTGAGCGCACTTAGGTATATACTGGCGCGCCGCGAAATCGGGCGACGATACTGAT
CACTTAATCAAGAAAGAGCC

Downstream 100 bases:

>100_bases
TCATCCCTAATCACTTGGAGTGGTTGCTTCAGGTGGCAAGGGATAACGAAAACGAGTGCTGGCAGTAGCCAGAAAAGGTG
CAGACCTTTTTCGCAGGGAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNNTRVIPARMFGRKASGGKLEV
LVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEEA
DKERYQTVYNQKPGAVAAPTAGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
TATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYE
HTMAAYEHAVKEQYRFFSYGDAMFIRKQMTKA

Sequences:

>Translated_352_residues
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNNTRVIPARMFGRKASGGKLEV
LVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEEA
DKERYQTVYNQKPGAVAAPTAGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
TATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYE
HTMAAYEHAVKEQYRFFSYGDAMFIRKQMTKA
>Mature_352_residues
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNNTRVIPARMFGRKASGGKLEV
LVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEEA
DKERYQTVYNQKPGAVAAPTAGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
TATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYE
HTMAAYEHAVKEQYRFFSYGDAMFIRKQMTKA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=72.463768115942, Blast_Score=527, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39458; Mature: 39458

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHHEEECCCCCCEECCHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKLEVLVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQ
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHH
ARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEEADKERYQTVYNQKPGAVAAPT
HHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
CCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
TATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLIT
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYEHTMAAYEHAVKEQYRFFSYGDAMFIRKQMTKA
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHHEEECCCCCCEECCHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKLEVLVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQ
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHH
ARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEEADKERYQTVYNQKPGAVAAPT
HHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
CCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
TATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLIT
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYEHTMAAYEHAVKEQYRFFSYGDAMFIRKQMTKA
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA