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The map label for this gene is mbaA

Identifier: 227080883

GI number: 227080883

Start: 708696

End: 711071

Strand: Reverse

Name: mbaA

Synonym: VCM66_0661

Alternate gene names: 227080883

Gene position: 711071-708696 (Counterclockwise)

Preceding gene: 227080884

Following gene: 227080882

Centisome position: 24.58

GC content: 45.24

Gene sequence:

>2376_bases
ATGAAGCTAAACCATAGAATTCTGTTACTGATTGCACCGGTGATCCTGCTCAGTGCAGCCGCATCAAGCTACATCATTTA
TACCAGTCAGAAAAATGCGCTGCTAAAAAGAACCGACAGTTACCTGCAGTTGAATATTGAAAAGTTAGCCAGCCACTATC
GACAAGCTCAGTCGCTCGTCAGCAGTTATGCCTTCACGCTGGCCAAAAGTGACATCATCCGCCACTACTTCTCTTTAGAA
AAAAATCCTTACCGCGAACTAGAACTGGTGGATAACTTACGTGAAACCCTGCAAATTTTGCAGCCGAATGAAAAACAGCT
CGTATCGTTATCGATCCTCAATGGCCATGAAGAGCTGCTTTATTATGCGGAAAATAGCTCCGATCCATTTGCCGAACTTG
ACCCTAAGGTGATGGCGTACATCAAACAGCGTTTTGCATCCACACAAAAAAACTCTGATATCAGCTACACCGTCAACTCA
GCGGGTGAAGATATTTTGGTTCGCTACGATATGCTGGATACTCAGACACTCTCAACGCCGCTCAGTTATAACCGCCAAGA
CGTGTTTTTTGTGGTGGTGTATGTGGTGCTTGAGCAGTTCAGCCAACTGCGCAAAAAAATTGAATTTGATAACCAGAGCC
CGATATTTTTTACGCACTCACCTCCGAGTTACCGCACGGGCTTGTTGCAATCGGTTGAATTACAACCGGGCTTTTACGCC
ATACTCGACCCTGCACCGAAACTGATTAACGCCCAGCTTCACTCCATTCAGCGCGAACTTTTACTCTCTTTTGGCGTTTC
GGCACTGGTGACGGTACTCATGCTACTGCTCCTTCTCTATCGCCATGTGATCAATCCGATCCTGCATTTGGATAAACAGC
TTGAAGAGGTGGAAAACAACCAACGTAAGAACATTGAAAAACTCAATACTGACGATGAAATTGGGCGGCTTTCCTCGCGT
TTTTACGCCATGTACAGCGAACTTCATTCAACCTATCAACGCACGAAAGCACTTGCGGAAAACGATCACCTCACCAAGCT
GGCTAACCGCTATCAATTTCAAGTTCAAGCCGATCTGCTGCTATCACGTTGTTACGATACTCAGCATATCTGGGTCATGT
ATATCGACTTAGATAACTTCAAGTACGTTAATGATAAGTATGGTCACCAAATCGGTGATTCCCTACTGGTCAGCTTCGCG
ACCCACGTTCGCCAGTTATGTAAAAATTTTGAGGCCAGCCATAACACCTACAGTATTGCGGCCCGTTTATCTGGTGATGA
GTTTGCTATTTTGCTCGTCTCCCCCAAACGGTTTAATGATTGTGCGAAGATCTTTGCTCAACGCTTGTTGGCACCGATCC
AAAATAAAGACAACTCACCACTTAGCCATTTTCCCATTACTGCCAGCATTGGTATTGCGACCTTCCCTAAAGATGGCGAA
CACATTGAGAAATTGCTGCTCAATGCCGATACCGCCATGTATCAAGCGAAAAATGCGGGCAAAAACCAAGTCGCTTACTA
TTCGCAGGCGTTAGATCAAATAGTACAGCGGCGCAATAATATTGAACGAGCACTGCGCTTAGGGTTATTTGATCAAGAGT
TCAATTTGGCTTATCAACCTTACTTTACTTGCTCAGGGAAACGCTTAGTGGGCTTCGAAGTATTGCTGCGCTGGCAATCT
GAATTGCTCGGTGAAGTGTCGCCGGAAGAGTTCATTCCAATTGCAGAGCAAACTGGATTGTTTGGCACGATCGACCGCTG
GGTCATTTCAAAAGCTTTTCAAGAGATATCAACTCTACAAGCCATAGTGAAAGAGCCGATCCAAGTGTCGATCAACCTCT
CTTCGGCAGAACTCAATTCGCTGAAATTGGCTCAATTTATCCATCGACAGGCTGAGCAGTTTGGCGTCTCCCCTGCATGG
ATCGATTTTGAAATCACCGAAACGTTTGCCGCAGATTCGCAAAGCTTCCCATTGCTGCACGAACTCTCGCGTCTTGGCTA
TGGGTTAACGATTGATGATTTTGGCTCAGGCTATACCTCTATTACTCAGTTGGTGCAGTATCCAGTACAAAAGATTAAGT
TTGATCGCCATTTTTTGGATACACTCATCGCCACCAACAAACAAAATGTTATTCGTCCACTGATTGATCTCTGCCACTCT
CAATCCATGAAAGTGACCGCTGAAGGCATTGAGAGTGAAACTATGCATCAATGGTTGGCCGATTATGAGTGCGATTACAT
GCAAGGCTTCTACTTTGGTTATCCAATGAGCCTAAGCGAGATCTCCCCATGGCTTCATGCCTCCAATCATAAAAAGAAGA
GTTATGCGCAAGATCATTATTGCTTCACAGAACCCAGCCAAAGTGAATGCCGTTAG

Upstream 100 bases:

>100_bases
CTCCCAGAGCAGCGATTGGCGGAGGGGATAATCGACAGCGAACTCTCGGCGAAAAACCTCAGTCTGCGTGCCAAAATCAT
CAGCAGTGTGACTTACCAAT

Downstream 100 bases:

>100_bases
AAGTGCCTTTAGCACCGTATTTCCAGATCAAGAATGGGAATTTATTGGCGTATCAGTACCGAGCGAAGTCGCCGATCAGC
CCATGAGTGACGAGGAAACC

Product: c-di-GMP phosphodiesterase A-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MKLNHRILLLIAPVILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLVSSYAFTLAKSDIIRHYFSLE
KNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELLYYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNS
AGEDILVRYDMLDTQTLSTPLSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENNQRKNIEKLNTDDEIGRLSSR
FYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLLLSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFA
THVRQLCKNFEASHNTYSIAARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQPYFTCSGKRLVGFEVLLRWQS
ELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQAIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAW
IDFEITETFAADSQSFPLLHELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHYCFTEPSQSECR

Sequences:

>Translated_791_residues
MKLNHRILLLIAPVILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLVSSYAFTLAKSDIIRHYFSLE
KNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELLYYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNS
AGEDILVRYDMLDTQTLSTPLSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENNQRKNIEKLNTDDEIGRLSSR
FYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLLLSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFA
THVRQLCKNFEASHNTYSIAARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQPYFTCSGKRLVGFEVLLRWQS
ELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQAIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAW
IDFEITETFAADSQSFPLLHELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHYCFTEPSQSECR
>Mature_791_residues
MKLNHRILLLIAPVILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLVSSYAFTLAKSDIIRHYFSLE
KNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELLYYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNS
AGEDILVRYDMLDTQTLSTPLSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENNQRKNIEKLNTDDEIGRLSSR
FYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLLLSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFA
THVRQLCKNFEASHNTYSIAARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQPYFTCSGKRLVGFEVLLRWQS
ELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQAIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAW
IDFEITETFAADSQSFPLLHELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHYCFTEPSQSECR

Specific function: Plays an essential role in the maintenance and the formation of the three-dimensional structure of the biofilms at the later stages of their development. Absence of mbaA promotes the accumulation of larger amount of biomass on the surfaces at later stage

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Single-pass type I membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain

Homologues:

Organism=Escherichia coli, GI1787541, Length=442, Percent_Identity=29.185520361991, Blast_Score=197, Evalue=3e-51,
Organism=Escherichia coli, GI87081921, Length=462, Percent_Identity=27.0562770562771, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=32.9268292682927, Blast_Score=140, Evalue=5e-34,
Organism=Escherichia coli, GI226510982, Length=653, Percent_Identity=23.1240428790199, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081743, Length=242, Percent_Identity=30.5785123966942, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788502, Length=248, Percent_Identity=25.8064516129032, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI1787055, Length=244, Percent_Identity=29.5081967213115, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI87081980, Length=254, Percent_Identity=28.3464566929134, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788849, Length=299, Percent_Identity=29.7658862876254, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87081845, Length=250, Percent_Identity=25.6, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786507, Length=247, Percent_Identity=23.0769230769231, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788381, Length=314, Percent_Identity=28.9808917197452, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082096, Length=200, Percent_Identity=28.5, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082007, Length=195, Percent_Identity=31.7948717948718, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=32.3353293413174, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1788085, Length=190, Percent_Identity=32.6315789473684, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=140, Percent_Identity=30.7142857142857, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=256, Percent_Identity=27.34375, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI1786584, Length=184, Percent_Identity=26.6304347826087, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=29.2817679558011, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1787816, Length=142, Percent_Identity=28.8732394366197, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=32.2784810126582, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBAA_VIBCH (Q9KU26)

Other databases:

- EMBL:   AE003852
- PIR:   A82291
- RefSeq:   NP_230352.1
- ProteinModelPortal:   Q9KU26
- GeneID:   2615707
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0703
- TIGR:   VC_0703
- HOGENOM:   HBG739657
- OMA:   MDHAVER
- ProtClustDB:   CLSK874076
- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR003660
- SMART:   SM00052
- SMART:   SM00267
- SMART:   SM00304
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00672 HAMP; SSF55073 A/G_cyclase

EC number: NA

Molecular weight: Translated: 90795; Mature: 90795

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50883 EAL; PS50887 GGDEF; PS50885 HAMP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x256ca8a8)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNHRILLLIAPVILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLV
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHEEEHHHHHHHHHHHHHHH
SSYAFTLAKSDIIRHYFSLEKNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCEE
YYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNSAGEDILVRYDMLDTQTLSTP
EEECCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCC
LSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHEECCCCEEE
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENN
EECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKNIEKLNTDDEIGRLSSRFYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHHHH
LSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFATHVRQLCKNFEASHNTYSIA
HHHHCCCCEEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEECCCCHH
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQP
HHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
YFTCSGKRLVGFEVLLRWQSELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQ
EEEECCCEEHHHHHHHHHHHHHHCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAWIDFEITETFAADSQSFPLLH
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCHHHH
ELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHY
CCCEEECCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCHHHCCHHHHCCCCCHHHHCCCCC
CFTEPSQSECR
CCCCCCCCCCH
>Mature Secondary Structure
MKLNHRILLLIAPVILLSAAASSYIIYTSQKNALLKRTDSYLQLNIEKLASHYRQAQSLV
CCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHEEEHHHHHHHHHHHHHHH
SSYAFTLAKSDIIRHYFSLEKNPYRELELVDNLRETLQILQPNEKQLVSLSILNGHEELL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCEE
YYAENSSDPFAELDPKVMAYIKQRFASTQKNSDISYTVNSAGEDILVRYDMLDTQTLSTP
EEECCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCHHHCCC
LSYNRQDVFFVVVYVVLEQFSQLRKKIEFDNQSPIFFTHSPPSYRTGLLQSVELQPGFYA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHEECCCCEEE
ILDPAPKLINAQLHSIQRELLLSFGVSALVTVLMLLLLLYRHVINPILHLDKQLEEVENN
EECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRKNIEKLNTDDEIGRLSSRFYAMYSELHSTYQRTKALAENDHLTKLANRYQFQVQADLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEHHHHHH
LSRCYDTQHIWVMYIDLDNFKYVNDKYGHQIGDSLLVSFATHVRQLCKNFEASHNTYSIA
HHHHCCCCEEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ARLSGDEFAILLVSPKRFNDCAKIFAQRLLAPIQNKDNSPLSHFPITASIGIATFPKDGE
EEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCEEECCCCHH
HIEKLLLNADTAMYQAKNAGKNQVAYYSQALDQIVQRRNNIERALRLGLFDQEFNLAYQP
HHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
YFTCSGKRLVGFEVLLRWQSELLGEVSPEEFIPIAEQTGLFGTIDRWVISKAFQEISTLQ
EEEECCCEEHHHHHHHHHHHHHHCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AIVKEPIQVSINLSSAELNSLKLAQFIHRQAEQFGVSPAWIDFEITETFAADSQSFPLLH
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCHHHH
ELSRLGYGLTIDDFGSGYTSITQLVQYPVQKIKFDRHFLDTLIATNKQNVIRPLIDLCHS
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
QSMKVTAEGIESETMHQWLADYECDYMQGFYFGYPMSLSEISPWLHASNHKKKSYAQDHY
CCCEEECCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCHHHCCHHHHCCCCCHHHHCCCCC
CFTEPSQSECR
CCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301